Role of bone marrow-derived mesenchymal stem cells on the parotid glands of streptozotocin induced diabetes rats
Background: The functional insufficiency of salivary glands constitute the common oral complaints in both type 1 and type 2 diabetes mellitus. The treatment with stem cell could decrease diabetic-induced hyposalivation and improve the quality of life for patients. Objective: The current study design...
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| Format: | Article |
| Language: | English |
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Elsevier
2020-04-01
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| Series: | Journal of Oral Biology and Craniofacial Research |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2212426820300130 |
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| author | Mona Denewar Laila E. Amin |
| author_facet | Mona Denewar Laila E. Amin |
| author_sort | Mona Denewar |
| collection | DOAJ |
| description | Background: The functional insufficiency of salivary glands constitute the common oral complaints in both type 1 and type 2 diabetes mellitus. The treatment with stem cell could decrease diabetic-induced hyposalivation and improve the quality of life for patients. Objective: The current study designed to assess the biological outcome of systemic injection of stem cells on the parotid salivary gland in streptozotocin induced diabetic. Methods: Twenty-four albino rats received intra-peritoneal injection of 50 mg/kg streptozotocin for induction of diabetes and were divided into two groups (n = 12): Group I (control) were kept without any manipulation, Group II received intravenous injection of 1×106 of mesenchymal stem cells of bone marrow derived for two days. All rats were sacrificed at 1, 3 weeks, then the parotid glands were isolated, fixed and processed for Heamatoxylin and Eosin examination, immunohistochemical staining for aquaporin-5. Results: group I groups showed intracellular cytoplasmic vacuoles and focal loss of salivary architecture, while group II showed maintenance of gland architecture. Immunohistochemical examination of aquaporin-5 showed significant difference between the two groups. Conclusion: bone marrow derived stem cell treatment considers as an improved methods in prevention and treatment of diabeticinduced hyposalivation. |
| format | Article |
| id | doaj-art-f9330f2fe2834f65926c2fb55dfb0b98 |
| institution | Kabale University |
| issn | 2212-4268 |
| language | English |
| publishDate | 2020-04-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Oral Biology and Craniofacial Research |
| spelling | doaj-art-f9330f2fe2834f65926c2fb55dfb0b982024-11-23T06:27:59ZengElsevierJournal of Oral Biology and Craniofacial Research2212-42682020-04-011023337Role of bone marrow-derived mesenchymal stem cells on the parotid glands of streptozotocin induced diabetes ratsMona Denewar0Laila E. Amin1Faculty of Dentistry, Mansoura University, EgyptFaculty of Dentistry, Mansoura University, Egypt; Faculty of Dentistry, Horus University, Egypt; Corresponding author. 35516, El-Gomhoria Street, Mansoura University, Egypt.Background: The functional insufficiency of salivary glands constitute the common oral complaints in both type 1 and type 2 diabetes mellitus. The treatment with stem cell could decrease diabetic-induced hyposalivation and improve the quality of life for patients. Objective: The current study designed to assess the biological outcome of systemic injection of stem cells on the parotid salivary gland in streptozotocin induced diabetic. Methods: Twenty-four albino rats received intra-peritoneal injection of 50 mg/kg streptozotocin for induction of diabetes and were divided into two groups (n = 12): Group I (control) were kept without any manipulation, Group II received intravenous injection of 1×106 of mesenchymal stem cells of bone marrow derived for two days. All rats were sacrificed at 1, 3 weeks, then the parotid glands were isolated, fixed and processed for Heamatoxylin and Eosin examination, immunohistochemical staining for aquaporin-5. Results: group I groups showed intracellular cytoplasmic vacuoles and focal loss of salivary architecture, while group II showed maintenance of gland architecture. Immunohistochemical examination of aquaporin-5 showed significant difference between the two groups. Conclusion: bone marrow derived stem cell treatment considers as an improved methods in prevention and treatment of diabeticinduced hyposalivation.http://www.sciencedirect.com/science/article/pii/S2212426820300130Stem cell therapyParotid salivary glandsDiabetes mellitusHyposalivationBone marrow derived stem cellAquaporin |
| spellingShingle | Mona Denewar Laila E. Amin Role of bone marrow-derived mesenchymal stem cells on the parotid glands of streptozotocin induced diabetes rats Journal of Oral Biology and Craniofacial Research Stem cell therapy Parotid salivary glands Diabetes mellitus Hyposalivation Bone marrow derived stem cell Aquaporin |
| title | Role of bone marrow-derived mesenchymal stem cells on the parotid glands of streptozotocin induced diabetes rats |
| title_full | Role of bone marrow-derived mesenchymal stem cells on the parotid glands of streptozotocin induced diabetes rats |
| title_fullStr | Role of bone marrow-derived mesenchymal stem cells on the parotid glands of streptozotocin induced diabetes rats |
| title_full_unstemmed | Role of bone marrow-derived mesenchymal stem cells on the parotid glands of streptozotocin induced diabetes rats |
| title_short | Role of bone marrow-derived mesenchymal stem cells on the parotid glands of streptozotocin induced diabetes rats |
| title_sort | role of bone marrow derived mesenchymal stem cells on the parotid glands of streptozotocin induced diabetes rats |
| topic | Stem cell therapy Parotid salivary glands Diabetes mellitus Hyposalivation Bone marrow derived stem cell Aquaporin |
| url | http://www.sciencedirect.com/science/article/pii/S2212426820300130 |
| work_keys_str_mv | AT monadenewar roleofbonemarrowderivedmesenchymalstemcellsontheparotidglandsofstreptozotocininduceddiabetesrats AT lailaeamin roleofbonemarrowderivedmesenchymalstemcellsontheparotidglandsofstreptozotocininduceddiabetesrats |