Comparison of retinol binding protein 1 with cone specific G-protein as putative effector molecules in cryptochrome signalling
Abstract Vision and magnetoreception in navigating songbirds are strongly connected as recent findings link a light dependent radical-pair mechanism in cryptochrome proteins to signalling pathways in cone photoreceptor cells. A previous yeast-two-hybrid screening approach identified six putative can...
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Nature Portfolio
2024-11-01
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| Series: | Scientific Reports |
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| Online Access: | https://doi.org/10.1038/s41598-024-79699-z |
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| author | Chad Yee Rabea Bartölke Katharina Görtemaker Jessica Schmidt Bo Leberecht Henrik Mouritsen Karl-Wilhelm Koch |
| author_facet | Chad Yee Rabea Bartölke Katharina Görtemaker Jessica Schmidt Bo Leberecht Henrik Mouritsen Karl-Wilhelm Koch |
| author_sort | Chad Yee |
| collection | DOAJ |
| description | Abstract Vision and magnetoreception in navigating songbirds are strongly connected as recent findings link a light dependent radical-pair mechanism in cryptochrome proteins to signalling pathways in cone photoreceptor cells. A previous yeast-two-hybrid screening approach identified six putative candidate proteins showing binding to cryptochrome type 4a. So far, only the interaction of the cone specific G-protein transducin α-subunit was investigated in more detail. In the present study, we compare the binding features of the G-protein α-subunit with those of another candidate from the yeast-two-hybrid screen, cellular retinol binding protein. Purified recombinant European robin retinol binding protein bound retinol with high affinity, displaying an EC50 of less than 5 nM, thereby demonstrating its functional state. We applied surface plasmon resonance and a Förster resonance transfer analysis to test for interactions between retinol binding protein and cryptochrome 4a. In the absence of retinol, we observed no robust binding events, which contrasts the strong interaction we observed between cryptochrome 4a and the G-protein α-subunit. We conclude that retinol binding protein is unlikely to be involved in the primary magnetosensory signalling cascade. |
| format | Article |
| id | doaj-art-f91e5d7a5fda4c809b70aa3987fd2500 |
| institution | Kabale University |
| issn | 2045-2322 |
| language | English |
| publishDate | 2024-11-01 |
| publisher | Nature Portfolio |
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| series | Scientific Reports |
| spelling | doaj-art-f91e5d7a5fda4c809b70aa3987fd25002024-11-17T12:24:01ZengNature PortfolioScientific Reports2045-23222024-11-0114111110.1038/s41598-024-79699-zComparison of retinol binding protein 1 with cone specific G-protein as putative effector molecules in cryptochrome signallingChad Yee0Rabea Bartölke1Katharina Görtemaker2Jessica Schmidt3Bo Leberecht4Henrik Mouritsen5Karl-Wilhelm Koch6Division of Biochemistry, Department of Neuroscience, Carl von Ossietzky Universität OldenburgNeurosensorics/Animal Navigation, Institute of Biology and Environmental Sciences, Carl von Ossietzky Universität OldenburgDivision of Biochemistry, Department of Neuroscience, Carl von Ossietzky Universität OldenburgNeurosensorics/Animal Navigation, Institute of Biology and Environmental Sciences, Carl von Ossietzky Universität OldenburgAnimal Biodiversity and Evolutionary Biology, Institute of Biology and Environmental Sciences, Carl von Ossietzky Universität OldenburgNeurosensorics/Animal Navigation, Institute of Biology and Environmental Sciences, Carl von Ossietzky Universität OldenburgDivision of Biochemistry, Department of Neuroscience, Carl von Ossietzky Universität OldenburgAbstract Vision and magnetoreception in navigating songbirds are strongly connected as recent findings link a light dependent radical-pair mechanism in cryptochrome proteins to signalling pathways in cone photoreceptor cells. A previous yeast-two-hybrid screening approach identified six putative candidate proteins showing binding to cryptochrome type 4a. So far, only the interaction of the cone specific G-protein transducin α-subunit was investigated in more detail. In the present study, we compare the binding features of the G-protein α-subunit with those of another candidate from the yeast-two-hybrid screen, cellular retinol binding protein. Purified recombinant European robin retinol binding protein bound retinol with high affinity, displaying an EC50 of less than 5 nM, thereby demonstrating its functional state. We applied surface plasmon resonance and a Förster resonance transfer analysis to test for interactions between retinol binding protein and cryptochrome 4a. In the absence of retinol, we observed no robust binding events, which contrasts the strong interaction we observed between cryptochrome 4a and the G-protein α-subunit. We conclude that retinol binding protein is unlikely to be involved in the primary magnetosensory signalling cascade.https://doi.org/10.1038/s41598-024-79699-zCryptochromeMagnetoreceptionRetinol binding proteinG proteinProtein–protein interaction |
| spellingShingle | Chad Yee Rabea Bartölke Katharina Görtemaker Jessica Schmidt Bo Leberecht Henrik Mouritsen Karl-Wilhelm Koch Comparison of retinol binding protein 1 with cone specific G-protein as putative effector molecules in cryptochrome signalling Scientific Reports Cryptochrome Magnetoreception Retinol binding protein G protein Protein–protein interaction |
| title | Comparison of retinol binding protein 1 with cone specific G-protein as putative effector molecules in cryptochrome signalling |
| title_full | Comparison of retinol binding protein 1 with cone specific G-protein as putative effector molecules in cryptochrome signalling |
| title_fullStr | Comparison of retinol binding protein 1 with cone specific G-protein as putative effector molecules in cryptochrome signalling |
| title_full_unstemmed | Comparison of retinol binding protein 1 with cone specific G-protein as putative effector molecules in cryptochrome signalling |
| title_short | Comparison of retinol binding protein 1 with cone specific G-protein as putative effector molecules in cryptochrome signalling |
| title_sort | comparison of retinol binding protein 1 with cone specific g protein as putative effector molecules in cryptochrome signalling |
| topic | Cryptochrome Magnetoreception Retinol binding protein G protein Protein–protein interaction |
| url | https://doi.org/10.1038/s41598-024-79699-z |
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