Efficacy and mechanism of cyprosulfamide in alleviating the phytotoxicity of clomazone residue on maize seedling
IntroductionThe residues of clomazone (Clo) can lead to phytotoxic symptoms such as foliar bleaching, reduced plant height, and decreased maize yields. Herbicide safener represent one of the most economically efficient strategies for mitigating herbicide-induced damage.MethodsIn this study, various...
Saved in:
| Main Authors: | , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Frontiers Media S.A.
2024-12-01
|
| Series: | Frontiers in Plant Science |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/articles/10.3389/fpls.2024.1512055/full |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1846114993197547520 |
|---|---|
| author | Lanlan Sun Lanlan Sun Lanlan Sun Chen Zhang Chen Zhang Chen Zhang Hongle Xu Hongle Xu Hongle Xu Wangcang Su Wangcang Su Wangcang Su Fei Xue Fei Xue Fei Xue Qiuli Leng Qiuli Leng Qiuli Leng Yujia Niu Yujia Niu Yujia Niu Chuantao Lu Chuantao Lu Chuantao Lu Renhai Wu Renhai Wu Renhai Wu |
| author_facet | Lanlan Sun Lanlan Sun Lanlan Sun Chen Zhang Chen Zhang Chen Zhang Hongle Xu Hongle Xu Hongle Xu Wangcang Su Wangcang Su Wangcang Su Fei Xue Fei Xue Fei Xue Qiuli Leng Qiuli Leng Qiuli Leng Yujia Niu Yujia Niu Yujia Niu Chuantao Lu Chuantao Lu Chuantao Lu Renhai Wu Renhai Wu Renhai Wu |
| author_sort | Lanlan Sun |
| collection | DOAJ |
| description | IntroductionThe residues of clomazone (Clo) can lead to phytotoxic symptoms such as foliar bleaching, reduced plant height, and decreased maize yields. Herbicide safener represent one of the most economically efficient strategies for mitigating herbicide-induced damage.MethodsIn this study, various seed treatments were implemented, including the immersion of maize seeds in water (CK), immersion in Cyprosulfamide (CSA), soil supplemented with clomazone (ClO) and CSA+ClO, evaluated physiological indicators, chlorophyll content, and qRT-PCR analyses of the maize plants were evaluated under the different treatments.Results and discussionThe objective of this study was to investigate the impact of CSA on mitigating residual damage caused by Clo on maize and elucidate its mechanism. Compared to the CK, treatment with Clo resulted in significant inhibition of maize plant height, fresh weight, chlorophyll content, and carotenoid levels by 19.0%, 29.9%, 92.5%, and 86.3% respectively. On the other hand, under CSA+Clo treatment, milder inhibition was observed with reductions of only 9.4% in plant height and 7.2% in fresh weight, as well as decreases of 35.7% and 21.8% respectively in chlorophyll and carotenoid contents. The findings revealed that the application of CSA effectively mitigated the inhibitory effects of Clo residues on maize plant height, fresh weight, carotenoids and chlorophyll content. Additionally, the combination of CSA and Clo reduced MDA levels by 13.4%, increased SOD activity by 9.7% and GST activity by 26.7%, while elevating GSSG content by 31.3% compared to Clo alone, ultimately mitigating oxidative damage in maize plants. qRT-PCR analysis showed that the expression of five P450 genes (CYP72A5, CYP81A4, CYP81Q32, CYP81A9, CYP81A36), nine GST genes (GST30, GST31, GSTIV, GSTVI, GST21, GST7, GST37, GST25, IN2-1), and two UGT genes (UGT76C2, UGT83A1) significantly high increased by 6.74-, 10.27-, 4.98-, 10.56-, 25.67-, 16.70-, 46.92-,7.53-, 5.10-, 238.82-, 143.50-, 4.58-, 31.51-, 39.3-, 4.20-, 10.47-fold after CSA+Clo treatment compared to that in the Clo treatment. The pre-treatment of CSA led to the upregulation of five P450 genes, nine GST genes, and two UGT genes, which may be associated with the metabolism of Clo in maize. Overall, this study suggests that CSA could be effectively mitigates Clo residual damage by up-regulating detoxification-related genes, enhancing chlorophyll content and activities of antioxidant enzymes. |
| format | Article |
| id | doaj-art-f8f906e1113249d6a52ecb9e8c42cbf4 |
| institution | Kabale University |
| issn | 1664-462X |
| language | English |
| publishDate | 2024-12-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Plant Science |
| spelling | doaj-art-f8f906e1113249d6a52ecb9e8c42cbf42024-12-20T05:10:16ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2024-12-011510.3389/fpls.2024.15120551512055Efficacy and mechanism of cyprosulfamide in alleviating the phytotoxicity of clomazone residue on maize seedlingLanlan Sun0Lanlan Sun1Lanlan Sun2Chen Zhang3Chen Zhang4Chen Zhang5Hongle Xu6Hongle Xu7Hongle Xu8Wangcang Su9Wangcang Su10Wangcang Su11Fei Xue12Fei Xue13Fei Xue14Qiuli Leng15Qiuli Leng16Qiuli Leng17Yujia Niu18Yujia Niu19Yujia Niu20Chuantao Lu21Chuantao Lu22Chuantao Lu23Renhai Wu24Renhai Wu25Renhai Wu26Institute of Plant Protection, Henan Academy of Agricultural Sciences, Zhengzhou, ChinaHenan Key Laboratory of Agricultural Pest Monitoring and Control, Zhengzhou, ChinaKey Laboratory for Integrated Crop Pests Management on Crops in Southern Region of North China, Zhengzhou, ChinaInstitute of Plant Protection, Henan Academy of Agricultural Sciences, Zhengzhou, ChinaHenan Key Laboratory of Agricultural Pest Monitoring and Control, Zhengzhou, ChinaKey Laboratory for Integrated Crop Pests Management on Crops in Southern Region of North China, Zhengzhou, ChinaInstitute of Plant Protection, Henan Academy of Agricultural Sciences, Zhengzhou, ChinaHenan Key Laboratory of Agricultural Pest Monitoring and Control, Zhengzhou, ChinaKey Laboratory for Integrated Crop Pests Management on Crops in Southern Region of North China, Zhengzhou, ChinaInstitute of Plant Protection, Henan Academy of Agricultural Sciences, Zhengzhou, ChinaHenan Key Laboratory of Agricultural Pest Monitoring and Control, Zhengzhou, ChinaKey Laboratory for Integrated Crop Pests Management on Crops in Southern Region of North China, Zhengzhou, ChinaInstitute of Plant Protection, Henan Academy of Agricultural Sciences, Zhengzhou, ChinaHenan Key Laboratory of Agricultural Pest Monitoring and Control, Zhengzhou, ChinaKey Laboratory for Integrated Crop Pests Management on Crops in Southern Region of North China, Zhengzhou, ChinaInstitute of Plant Protection, Henan Academy of Agricultural Sciences, Zhengzhou, ChinaHenan Key Laboratory of Agricultural Pest Monitoring and Control, Zhengzhou, ChinaKey Laboratory for Integrated Crop Pests Management on Crops in Southern Region of North China, Zhengzhou, ChinaInstitute of Plant Protection, Henan Academy of Agricultural Sciences, Zhengzhou, ChinaHenan Key Laboratory of Agricultural Pest Monitoring and Control, Zhengzhou, ChinaKey Laboratory for Integrated Crop Pests Management on Crops in Southern Region of North China, Zhengzhou, ChinaInstitute of Plant Protection, Henan Academy of Agricultural Sciences, Zhengzhou, ChinaHenan Key Laboratory of Agricultural Pest Monitoring and Control, Zhengzhou, ChinaKey Laboratory for Integrated Crop Pests Management on Crops in Southern Region of North China, Zhengzhou, ChinaInstitute of Plant Protection, Henan Academy of Agricultural Sciences, Zhengzhou, ChinaHenan Key Laboratory of Agricultural Pest Monitoring and Control, Zhengzhou, ChinaKey Laboratory for Integrated Crop Pests Management on Crops in Southern Region of North China, Zhengzhou, ChinaIntroductionThe residues of clomazone (Clo) can lead to phytotoxic symptoms such as foliar bleaching, reduced plant height, and decreased maize yields. Herbicide safener represent one of the most economically efficient strategies for mitigating herbicide-induced damage.MethodsIn this study, various seed treatments were implemented, including the immersion of maize seeds in water (CK), immersion in Cyprosulfamide (CSA), soil supplemented with clomazone (ClO) and CSA+ClO, evaluated physiological indicators, chlorophyll content, and qRT-PCR analyses of the maize plants were evaluated under the different treatments.Results and discussionThe objective of this study was to investigate the impact of CSA on mitigating residual damage caused by Clo on maize and elucidate its mechanism. Compared to the CK, treatment with Clo resulted in significant inhibition of maize plant height, fresh weight, chlorophyll content, and carotenoid levels by 19.0%, 29.9%, 92.5%, and 86.3% respectively. On the other hand, under CSA+Clo treatment, milder inhibition was observed with reductions of only 9.4% in plant height and 7.2% in fresh weight, as well as decreases of 35.7% and 21.8% respectively in chlorophyll and carotenoid contents. The findings revealed that the application of CSA effectively mitigated the inhibitory effects of Clo residues on maize plant height, fresh weight, carotenoids and chlorophyll content. Additionally, the combination of CSA and Clo reduced MDA levels by 13.4%, increased SOD activity by 9.7% and GST activity by 26.7%, while elevating GSSG content by 31.3% compared to Clo alone, ultimately mitigating oxidative damage in maize plants. qRT-PCR analysis showed that the expression of five P450 genes (CYP72A5, CYP81A4, CYP81Q32, CYP81A9, CYP81A36), nine GST genes (GST30, GST31, GSTIV, GSTVI, GST21, GST7, GST37, GST25, IN2-1), and two UGT genes (UGT76C2, UGT83A1) significantly high increased by 6.74-, 10.27-, 4.98-, 10.56-, 25.67-, 16.70-, 46.92-,7.53-, 5.10-, 238.82-, 143.50-, 4.58-, 31.51-, 39.3-, 4.20-, 10.47-fold after CSA+Clo treatment compared to that in the Clo treatment. The pre-treatment of CSA led to the upregulation of five P450 genes, nine GST genes, and two UGT genes, which may be associated with the metabolism of Clo in maize. Overall, this study suggests that CSA could be effectively mitigates Clo residual damage by up-regulating detoxification-related genes, enhancing chlorophyll content and activities of antioxidant enzymes.https://www.frontiersin.org/articles/10.3389/fpls.2024.1512055/fullantioxidationcarotenoidgene expressionClomazone residuephytotoxicity |
| spellingShingle | Lanlan Sun Lanlan Sun Lanlan Sun Chen Zhang Chen Zhang Chen Zhang Hongle Xu Hongle Xu Hongle Xu Wangcang Su Wangcang Su Wangcang Su Fei Xue Fei Xue Fei Xue Qiuli Leng Qiuli Leng Qiuli Leng Yujia Niu Yujia Niu Yujia Niu Chuantao Lu Chuantao Lu Chuantao Lu Renhai Wu Renhai Wu Renhai Wu Efficacy and mechanism of cyprosulfamide in alleviating the phytotoxicity of clomazone residue on maize seedling Frontiers in Plant Science antioxidation carotenoid gene expression Clomazone residue phytotoxicity |
| title | Efficacy and mechanism of cyprosulfamide in alleviating the phytotoxicity of clomazone residue on maize seedling |
| title_full | Efficacy and mechanism of cyprosulfamide in alleviating the phytotoxicity of clomazone residue on maize seedling |
| title_fullStr | Efficacy and mechanism of cyprosulfamide in alleviating the phytotoxicity of clomazone residue on maize seedling |
| title_full_unstemmed | Efficacy and mechanism of cyprosulfamide in alleviating the phytotoxicity of clomazone residue on maize seedling |
| title_short | Efficacy and mechanism of cyprosulfamide in alleviating the phytotoxicity of clomazone residue on maize seedling |
| title_sort | efficacy and mechanism of cyprosulfamide in alleviating the phytotoxicity of clomazone residue on maize seedling |
| topic | antioxidation carotenoid gene expression Clomazone residue phytotoxicity |
| url | https://www.frontiersin.org/articles/10.3389/fpls.2024.1512055/full |
| work_keys_str_mv | AT lanlansun efficacyandmechanismofcyprosulfamideinalleviatingthephytotoxicityofclomazoneresidueonmaizeseedling AT lanlansun efficacyandmechanismofcyprosulfamideinalleviatingthephytotoxicityofclomazoneresidueonmaizeseedling AT lanlansun efficacyandmechanismofcyprosulfamideinalleviatingthephytotoxicityofclomazoneresidueonmaizeseedling AT chenzhang efficacyandmechanismofcyprosulfamideinalleviatingthephytotoxicityofclomazoneresidueonmaizeseedling AT chenzhang efficacyandmechanismofcyprosulfamideinalleviatingthephytotoxicityofclomazoneresidueonmaizeseedling AT chenzhang efficacyandmechanismofcyprosulfamideinalleviatingthephytotoxicityofclomazoneresidueonmaizeseedling AT honglexu efficacyandmechanismofcyprosulfamideinalleviatingthephytotoxicityofclomazoneresidueonmaizeseedling AT honglexu efficacyandmechanismofcyprosulfamideinalleviatingthephytotoxicityofclomazoneresidueonmaizeseedling AT honglexu efficacyandmechanismofcyprosulfamideinalleviatingthephytotoxicityofclomazoneresidueonmaizeseedling AT wangcangsu efficacyandmechanismofcyprosulfamideinalleviatingthephytotoxicityofclomazoneresidueonmaizeseedling AT wangcangsu efficacyandmechanismofcyprosulfamideinalleviatingthephytotoxicityofclomazoneresidueonmaizeseedling AT wangcangsu efficacyandmechanismofcyprosulfamideinalleviatingthephytotoxicityofclomazoneresidueonmaizeseedling AT feixue efficacyandmechanismofcyprosulfamideinalleviatingthephytotoxicityofclomazoneresidueonmaizeseedling AT feixue efficacyandmechanismofcyprosulfamideinalleviatingthephytotoxicityofclomazoneresidueonmaizeseedling AT feixue efficacyandmechanismofcyprosulfamideinalleviatingthephytotoxicityofclomazoneresidueonmaizeseedling AT qiulileng efficacyandmechanismofcyprosulfamideinalleviatingthephytotoxicityofclomazoneresidueonmaizeseedling AT qiulileng efficacyandmechanismofcyprosulfamideinalleviatingthephytotoxicityofclomazoneresidueonmaizeseedling AT qiulileng efficacyandmechanismofcyprosulfamideinalleviatingthephytotoxicityofclomazoneresidueonmaizeseedling AT yujianiu efficacyandmechanismofcyprosulfamideinalleviatingthephytotoxicityofclomazoneresidueonmaizeseedling AT yujianiu efficacyandmechanismofcyprosulfamideinalleviatingthephytotoxicityofclomazoneresidueonmaizeseedling AT yujianiu efficacyandmechanismofcyprosulfamideinalleviatingthephytotoxicityofclomazoneresidueonmaizeseedling AT chuantaolu efficacyandmechanismofcyprosulfamideinalleviatingthephytotoxicityofclomazoneresidueonmaizeseedling AT chuantaolu efficacyandmechanismofcyprosulfamideinalleviatingthephytotoxicityofclomazoneresidueonmaizeseedling AT chuantaolu efficacyandmechanismofcyprosulfamideinalleviatingthephytotoxicityofclomazoneresidueonmaizeseedling AT renhaiwu efficacyandmechanismofcyprosulfamideinalleviatingthephytotoxicityofclomazoneresidueonmaizeseedling AT renhaiwu efficacyandmechanismofcyprosulfamideinalleviatingthephytotoxicityofclomazoneresidueonmaizeseedling AT renhaiwu efficacyandmechanismofcyprosulfamideinalleviatingthephytotoxicityofclomazoneresidueonmaizeseedling |