Deciphering the Molecular Mechanism of Peracetic Acid Response in Listeria monocytogenes
Peracetic acid (PAA), a strong oxidizing agent, has been widely used as a disinfectant in food processing settings as it does not produce harmful chlorinated by-products. In the present study, the transcriptional response of Listeria monocytogenes to a sub-lethal concentration of PAA (2.5 ppm) was a...
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Elsevier
2025-01-01
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| Series: | Journal of Food Protection |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S0362028X24001856 |
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| author | Marianna Arvaniti Ahmed Gaballa Renato H. Orsi Panagiotis Skandamis Martin Wiedmann |
| author_facet | Marianna Arvaniti Ahmed Gaballa Renato H. Orsi Panagiotis Skandamis Martin Wiedmann |
| author_sort | Marianna Arvaniti |
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| description | Peracetic acid (PAA), a strong oxidizing agent, has been widely used as a disinfectant in food processing settings as it does not produce harmful chlorinated by-products. In the present study, the transcriptional response of Listeria monocytogenes to a sub-lethal concentration of PAA (2.5 ppm) was assessed using RNA-sequencing (RNA-seq). Our analysis revealed 12 differentially expressed protein-coding genes, of which nine were upregulated (ohrR, ohrA, rpsN, lmo0637, lmo1973, fur, lmo2492, zurM, and lmo1007), and three were down-regulated (argG, lmo0604 and lmo2156) in PAA-treated samples compared to the control samples. A non-coding small RNA gene (rli32) was also found to be down-regulated. In detail, the organic peroxide toxicity protection (OhrA-OhrR) system, the metal homeostasis genes fur and zurM, the SbrE-regulated lmo0636-lmo0637 operon and a carbohydrate phosphotransferase system (PTS) operon component were induced under exposure of L. monocytogenes to PAA. Hence, this study identified key elements involved in the primary response of L. monocytogenes to oxidative stress caused by PAA, including the expression of the peroxide detoxification system and fine-tuning the levels of redox-active metals in the cell. The investigation of the molecular mechanism of PAA response in L. monocytogenes is of utmost importance for the food industry, as residual PAA can lead to stress tolerance in pathogens. |
| format | Article |
| id | doaj-art-f865ab1053da4c71b9f629ed545624fa |
| institution | Kabale University |
| issn | 0362-028X |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Elsevier |
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| series | Journal of Food Protection |
| spelling | doaj-art-f865ab1053da4c71b9f629ed545624fa2025-01-09T06:12:30ZengElsevierJournal of Food Protection0362-028X2025-01-01881100401Deciphering the Molecular Mechanism of Peracetic Acid Response in Listeria monocytogenesMarianna Arvaniti0Ahmed Gaballa1Renato H. Orsi2Panagiotis Skandamis3Martin Wiedmann4Laboratory of Food Quality Control and Hygiene, Department of Food Science and Human Nutrition, Agricultural University of Athens, Athens, Greece; Corresponding author.Food Safety Laboratory, Department of Food Science, Cornell University, Ithaca, NY 14853, USAFood Safety Laboratory, Department of Food Science, Cornell University, Ithaca, NY 14853, USALaboratory of Food Quality Control and Hygiene, Department of Food Science and Human Nutrition, Agricultural University of Athens, Athens, GreeceFood Safety Laboratory, Department of Food Science, Cornell University, Ithaca, NY 14853, USAPeracetic acid (PAA), a strong oxidizing agent, has been widely used as a disinfectant in food processing settings as it does not produce harmful chlorinated by-products. In the present study, the transcriptional response of Listeria monocytogenes to a sub-lethal concentration of PAA (2.5 ppm) was assessed using RNA-sequencing (RNA-seq). Our analysis revealed 12 differentially expressed protein-coding genes, of which nine were upregulated (ohrR, ohrA, rpsN, lmo0637, lmo1973, fur, lmo2492, zurM, and lmo1007), and three were down-regulated (argG, lmo0604 and lmo2156) in PAA-treated samples compared to the control samples. A non-coding small RNA gene (rli32) was also found to be down-regulated. In detail, the organic peroxide toxicity protection (OhrA-OhrR) system, the metal homeostasis genes fur and zurM, the SbrE-regulated lmo0636-lmo0637 operon and a carbohydrate phosphotransferase system (PTS) operon component were induced under exposure of L. monocytogenes to PAA. Hence, this study identified key elements involved in the primary response of L. monocytogenes to oxidative stress caused by PAA, including the expression of the peroxide detoxification system and fine-tuning the levels of redox-active metals in the cell. The investigation of the molecular mechanism of PAA response in L. monocytogenes is of utmost importance for the food industry, as residual PAA can lead to stress tolerance in pathogens.http://www.sciencedirect.com/science/article/pii/S0362028X24001856Listeria monocytogenesOhrA-OhrR systemOxidative stress responsePeracetic acidRNA-seq |
| spellingShingle | Marianna Arvaniti Ahmed Gaballa Renato H. Orsi Panagiotis Skandamis Martin Wiedmann Deciphering the Molecular Mechanism of Peracetic Acid Response in Listeria monocytogenes Journal of Food Protection Listeria monocytogenes OhrA-OhrR system Oxidative stress response Peracetic acid RNA-seq |
| title | Deciphering the Molecular Mechanism of Peracetic Acid Response in Listeria monocytogenes |
| title_full | Deciphering the Molecular Mechanism of Peracetic Acid Response in Listeria monocytogenes |
| title_fullStr | Deciphering the Molecular Mechanism of Peracetic Acid Response in Listeria monocytogenes |
| title_full_unstemmed | Deciphering the Molecular Mechanism of Peracetic Acid Response in Listeria monocytogenes |
| title_short | Deciphering the Molecular Mechanism of Peracetic Acid Response in Listeria monocytogenes |
| title_sort | deciphering the molecular mechanism of peracetic acid response in listeria monocytogenes |
| topic | Listeria monocytogenes OhrA-OhrR system Oxidative stress response Peracetic acid RNA-seq |
| url | http://www.sciencedirect.com/science/article/pii/S0362028X24001856 |
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