3D culture inhibits replicative senescence of SCAPs via UQCRC2-mediated mitochondrial oxidative phosphorylation
Abstract Stem cells derived from the apical papilla (SCAPs) play a crucial role in tooth root development and dental pulp regeneration. They are important seed cells for bone/tooth tissue engineering. However, replicative senescence remains an unavoidable issue as in vitro amplification increases. T...
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| Format: | Article |
| Language: | English |
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BMC
2024-12-01
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| Series: | Journal of Translational Medicine |
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| Online Access: | https://doi.org/10.1186/s12967-024-05953-7 |
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| author | Yijia Shi Tong Xiao Yingying Weng Ya Xiao Jintao Wu Jing Wang Wenmin Wang Maoshen Yan Ming Yan Zehan Li Jinhua Yu |
| author_facet | Yijia Shi Tong Xiao Yingying Weng Ya Xiao Jintao Wu Jing Wang Wenmin Wang Maoshen Yan Ming Yan Zehan Li Jinhua Yu |
| author_sort | Yijia Shi |
| collection | DOAJ |
| description | Abstract Stem cells derived from the apical papilla (SCAPs) play a crucial role in tooth root development and dental pulp regeneration. They are important seed cells for bone/tooth tissue engineering. However, replicative senescence remains an unavoidable issue as in vitro amplification increases. This study investigated the effect of a three-dimensional (3D) culture environment constructed with methylcellulose on SCAPs senescence. It was observed that 3D culture conditions can delay cellular senescence, potentially due to changes in mitochondrial function and oxidative phosphorylation. Transcriptome high-throughput sequencing technology revealed that the different mitochondrial states may be related to UQCRC2. Knocking down UQCRC2 expression in the 3D culture group resulted in increased production of mitochondrial reactive oxygen species, decreased mitochondrial membrane potential, and a decline in the oxygen consumption rate for oxidative phosphorylation, accelerating cell senescence. The results of this study indicated that 3D culture can mitigate SCAPs aging by maintaining UQCRC2-mediated mitochondrial homeostasis. These findings provide a new solution for the senescence of SCAPs during in vitro amplification and can promote the applications of SCAPs-based clinical translation. |
| format | Article |
| id | doaj-art-f81a7044404f4497b31873c275e12b3b |
| institution | Kabale University |
| issn | 1479-5876 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | BMC |
| record_format | Article |
| series | Journal of Translational Medicine |
| spelling | doaj-art-f81a7044404f4497b31873c275e12b3b2024-12-22T12:44:26ZengBMCJournal of Translational Medicine1479-58762024-12-0122111510.1186/s12967-024-05953-73D culture inhibits replicative senescence of SCAPs via UQCRC2-mediated mitochondrial oxidative phosphorylationYijia Shi0Tong Xiao1Yingying Weng2Ya Xiao3Jintao Wu4Jing Wang5Wenmin Wang6Maoshen Yan7Ming Yan8Zehan Li9Jinhua Yu10Department of Endodontics, The Affiliated Stomatological Hospital of Nanjing Medical UniversityDepartment of Endodontics, The Affiliated Stomatological Hospital of Nanjing Medical UniversityDepartment of Endodontics, The Affiliated Stomatological Hospital of Nanjing Medical UniversityCollege & Hospital of Stomatology, Key Laboratory of Oral Diseases Research of Anhui Province, Anhui Medical UniversityDepartment of Endodontics, The Affiliated Stomatological Hospital of Nanjing Medical UniversityDepartment of Endodontics, The Affiliated Stomatological Hospital of Nanjing Medical UniversityDepartment of Endodontics, The Affiliated Stomatological Hospital of Nanjing Medical UniversityDepartment of Endodontics, The Affiliated Stomatological Hospital of Nanjing Medical UniversityDepartment of Endodontics, The Affiliated Stomatological Hospital of Nanjing Medical UniversityDepartment of Endodontics, The Affiliated Stomatological Hospital of Nanjing Medical UniversityDepartment of Endodontics, The Affiliated Stomatological Hospital of Nanjing Medical UniversityAbstract Stem cells derived from the apical papilla (SCAPs) play a crucial role in tooth root development and dental pulp regeneration. They are important seed cells for bone/tooth tissue engineering. However, replicative senescence remains an unavoidable issue as in vitro amplification increases. This study investigated the effect of a three-dimensional (3D) culture environment constructed with methylcellulose on SCAPs senescence. It was observed that 3D culture conditions can delay cellular senescence, potentially due to changes in mitochondrial function and oxidative phosphorylation. Transcriptome high-throughput sequencing technology revealed that the different mitochondrial states may be related to UQCRC2. Knocking down UQCRC2 expression in the 3D culture group resulted in increased production of mitochondrial reactive oxygen species, decreased mitochondrial membrane potential, and a decline in the oxygen consumption rate for oxidative phosphorylation, accelerating cell senescence. The results of this study indicated that 3D culture can mitigate SCAPs aging by maintaining UQCRC2-mediated mitochondrial homeostasis. These findings provide a new solution for the senescence of SCAPs during in vitro amplification and can promote the applications of SCAPs-based clinical translation.https://doi.org/10.1186/s12967-024-05953-7SCAPs3D cultureMitochondriaEnergy metabolismUQCRC2 |
| spellingShingle | Yijia Shi Tong Xiao Yingying Weng Ya Xiao Jintao Wu Jing Wang Wenmin Wang Maoshen Yan Ming Yan Zehan Li Jinhua Yu 3D culture inhibits replicative senescence of SCAPs via UQCRC2-mediated mitochondrial oxidative phosphorylation Journal of Translational Medicine SCAPs 3D culture Mitochondria Energy metabolism UQCRC2 |
| title | 3D culture inhibits replicative senescence of SCAPs via UQCRC2-mediated mitochondrial oxidative phosphorylation |
| title_full | 3D culture inhibits replicative senescence of SCAPs via UQCRC2-mediated mitochondrial oxidative phosphorylation |
| title_fullStr | 3D culture inhibits replicative senescence of SCAPs via UQCRC2-mediated mitochondrial oxidative phosphorylation |
| title_full_unstemmed | 3D culture inhibits replicative senescence of SCAPs via UQCRC2-mediated mitochondrial oxidative phosphorylation |
| title_short | 3D culture inhibits replicative senescence of SCAPs via UQCRC2-mediated mitochondrial oxidative phosphorylation |
| title_sort | 3d culture inhibits replicative senescence of scaps via uqcrc2 mediated mitochondrial oxidative phosphorylation |
| topic | SCAPs 3D culture Mitochondria Energy metabolism UQCRC2 |
| url | https://doi.org/10.1186/s12967-024-05953-7 |
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