Effect of replacing fishmeal with 70 cottonseed protein concentrate on growth performance, liver health, muscle texture, intestinal microbiome, and wastewater parameters in hybrid grouper (Epinephelus fuscoguttatus♀ × E. lanceolatus♂) juvenile
As a superior protein historically applied in aquaculture, cottonseed protein has been processed by various deep processing methods to improve its utilization in aquafeeds. In this study, 70 cottonseed protein concentrate with low-gossypol (70 C) was used to replace fishmeal (20 %, 40 %, 60 %, and 8...
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Elsevier
2025-09-01
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| Series: | Aquaculture Reports |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2352513425003230 |
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| author | Kangze Xv Adinda Luthfiah Min Xue Hao Wang Tingting Wang Xin Zhang Dengbao Fan Beiping Tan Wei Zhang |
| author_facet | Kangze Xv Adinda Luthfiah Min Xue Hao Wang Tingting Wang Xin Zhang Dengbao Fan Beiping Tan Wei Zhang |
| author_sort | Kangze Xv |
| collection | DOAJ |
| description | As a superior protein historically applied in aquaculture, cottonseed protein has been processed by various deep processing methods to improve its utilization in aquafeeds. In this study, 70 cottonseed protein concentrate with low-gossypol (70 C) was used to replace fishmeal (20 %, 40 %, 60 %, and 80 %) to evaluate the effect on hybrid grouper (Epinephelus fuscoguttatus♀ × E. lanceolatus♂) juveniles (15.00 ± 0.05 g). After a 7-week feeding trial, 40 % 70 C replacing fishmeal had no negative impact on the growth performance of juveniles (P > 0.05). Serum biochemical indices, including alanine aminotransferase (ALT), albumin (ALB), and total cholesterol (TC), were significantly reduced with an increase in dietary 70 C (P < 0.05), suggesting that liver function was impaired, which was further confirmed by the alteration of hepatic morphology. Moreover, the core microbiota of the intestine were majorly Proteobacteria, Firmicutes, Cyanobacteria, Bacteroidota, and Actinobacteriota, etc. at the Phylum level. At the Genus level, Enterobacter significantly decreased with increasing dietary 70 C (P < 0.05). NS3a_marine_group, Sphingomonas Amaricoccus, Craurococcus_Caldovatus, and Agrococcus achieved the highest abundance, whereas Nocardioides and Lachnoclostridium had the lowest abundance in the 70 C20 group. Furthermore, the abundance of Photobacterium and Amaricoccus was more overrepresented in the 70 C60 group than in the other two groups (P < 0.05). The abundance of the predicted pathways related to butanoate metabolism increased in the 70 C group compared to the FM group. Thus, we hypothesized that dietary 70 C would influence the composition of the intestinal microbiota, thereby affecting flesh texture and degrading water quality. Our research provides a theoretical basis for widening the application of cotton protein concentrates in grouper aquafeeds. |
| format | Article |
| id | doaj-art-f7e7573a897c48f688ed6de09a38208a |
| institution | Kabale University |
| issn | 2352-5134 |
| language | English |
| publishDate | 2025-09-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Aquaculture Reports |
| spelling | doaj-art-f7e7573a897c48f688ed6de09a38208a2025-08-20T04:00:32ZengElsevierAquaculture Reports2352-51342025-09-014310293710.1016/j.aqrep.2025.102937Effect of replacing fishmeal with 70 cottonseed protein concentrate on growth performance, liver health, muscle texture, intestinal microbiome, and wastewater parameters in hybrid grouper (Epinephelus fuscoguttatus♀ × E. lanceolatus♂) juvenileKangze Xv0Adinda Luthfiah1Min Xue2Hao Wang3Tingting Wang4Xin Zhang5Dengbao Fan6Beiping Tan7Wei Zhang8Laboratory of Aquatic Animal Nutrition and Feed, College of Fisheries, Guangdong Ocean University, Zhanjiang, Guangdong 524088, China; Aquatic Animals Precision Nutrition and High Efficiency Feed Engineering Research Center of Guangdong Province, Zhanjiang, Guangdong 524088, China; Key Laboratory of Aquatic, Livestock and Poultry Feed Science and Technology in South China, Ministry of Agriculture, Zhanjiang, Guangdong 524088, ChinaLaboratory of Aquatic Animal Nutrition and Feed, College of Fisheries, Guangdong Ocean University, Zhanjiang, Guangdong 524088, China; Aquatic Animals Precision Nutrition and High Efficiency Feed Engineering Research Center of Guangdong Province, Zhanjiang, Guangdong 524088, China; Key Laboratory of Aquatic, Livestock and Poultry Feed Science and Technology in South China, Ministry of Agriculture, Zhanjiang, Guangdong 524088, ChinaInstitute of Feed Research, Chinese Academy of Agricultural Sciences, Beijing 100081, ChinaInstitute of Feed Research, Chinese Academy of Agricultural Sciences, Beijing 100081, ChinaLaboratory of Aquatic Animal Nutrition and Feed, College of Fisheries, Guangdong Ocean University, Zhanjiang, Guangdong 524088, China; Aquatic Animals Precision Nutrition and High Efficiency Feed Engineering Research Center of Guangdong Province, Zhanjiang, Guangdong 524088, China; Key Laboratory of Aquatic, Livestock and Poultry Feed Science and Technology in South China, Ministry of Agriculture, Zhanjiang, Guangdong 524088, ChinaLaboratory of Aquatic Animal Nutrition and Feed, College of Fisheries, Guangdong Ocean University, Zhanjiang, Guangdong 524088, China; Aquatic Animals Precision Nutrition and High Efficiency Feed Engineering Research Center of Guangdong Province, Zhanjiang, Guangdong 524088, China; Key Laboratory of Aquatic, Livestock and Poultry Feed Science and Technology in South China, Ministry of Agriculture, Zhanjiang, Guangdong 524088, ChinaLaboratory of Aquatic Animal Nutrition and Feed, College of Fisheries, Guangdong Ocean University, Zhanjiang, Guangdong 524088, China; Aquatic Animals Precision Nutrition and High Efficiency Feed Engineering Research Center of Guangdong Province, Zhanjiang, Guangdong 524088, China; Key Laboratory of Aquatic, Livestock and Poultry Feed Science and Technology in South China, Ministry of Agriculture, Zhanjiang, Guangdong 524088, ChinaLaboratory of Aquatic Animal Nutrition and Feed, College of Fisheries, Guangdong Ocean University, Zhanjiang, Guangdong 524088, China; Aquatic Animals Precision Nutrition and High Efficiency Feed Engineering Research Center of Guangdong Province, Zhanjiang, Guangdong 524088, China; Key Laboratory of Aquatic, Livestock and Poultry Feed Science and Technology in South China, Ministry of Agriculture, Zhanjiang, Guangdong 524088, China; Corresponding authors at: Laboratory of Aquatic Animal Nutrition and Feed, College of Fisheries, Guangdong Ocean University, Zhanjiang, Guangdong 524088, China.Laboratory of Aquatic Animal Nutrition and Feed, College of Fisheries, Guangdong Ocean University, Zhanjiang, Guangdong 524088, China; Aquatic Animals Precision Nutrition and High Efficiency Feed Engineering Research Center of Guangdong Province, Zhanjiang, Guangdong 524088, China; Key Laboratory of Aquatic, Livestock and Poultry Feed Science and Technology in South China, Ministry of Agriculture, Zhanjiang, Guangdong 524088, China; Guangdong Provincial Key Laboratory of Aquatic Animal Disease Control and Healthy Culture, Zhanjiang, Guangdong 524088, China; Corresponding authors at: Laboratory of Aquatic Animal Nutrition and Feed, College of Fisheries, Guangdong Ocean University, Zhanjiang, Guangdong 524088, China.As a superior protein historically applied in aquaculture, cottonseed protein has been processed by various deep processing methods to improve its utilization in aquafeeds. In this study, 70 cottonseed protein concentrate with low-gossypol (70 C) was used to replace fishmeal (20 %, 40 %, 60 %, and 80 %) to evaluate the effect on hybrid grouper (Epinephelus fuscoguttatus♀ × E. lanceolatus♂) juveniles (15.00 ± 0.05 g). After a 7-week feeding trial, 40 % 70 C replacing fishmeal had no negative impact on the growth performance of juveniles (P > 0.05). Serum biochemical indices, including alanine aminotransferase (ALT), albumin (ALB), and total cholesterol (TC), were significantly reduced with an increase in dietary 70 C (P < 0.05), suggesting that liver function was impaired, which was further confirmed by the alteration of hepatic morphology. Moreover, the core microbiota of the intestine were majorly Proteobacteria, Firmicutes, Cyanobacteria, Bacteroidota, and Actinobacteriota, etc. at the Phylum level. At the Genus level, Enterobacter significantly decreased with increasing dietary 70 C (P < 0.05). NS3a_marine_group, Sphingomonas Amaricoccus, Craurococcus_Caldovatus, and Agrococcus achieved the highest abundance, whereas Nocardioides and Lachnoclostridium had the lowest abundance in the 70 C20 group. Furthermore, the abundance of Photobacterium and Amaricoccus was more overrepresented in the 70 C60 group than in the other two groups (P < 0.05). The abundance of the predicted pathways related to butanoate metabolism increased in the 70 C group compared to the FM group. Thus, we hypothesized that dietary 70 C would influence the composition of the intestinal microbiota, thereby affecting flesh texture and degrading water quality. Our research provides a theoretical basis for widening the application of cotton protein concentrates in grouper aquafeeds.http://www.sciencedirect.com/science/article/pii/S2352513425003230Hybrid grouperEpinephelus fuscoguttatus♀ × E. lanceolatus♂Fishmeal substituteCottonseed protein concentrateDeep processing |
| spellingShingle | Kangze Xv Adinda Luthfiah Min Xue Hao Wang Tingting Wang Xin Zhang Dengbao Fan Beiping Tan Wei Zhang Effect of replacing fishmeal with 70 cottonseed protein concentrate on growth performance, liver health, muscle texture, intestinal microbiome, and wastewater parameters in hybrid grouper (Epinephelus fuscoguttatus♀ × E. lanceolatus♂) juvenile Aquaculture Reports Hybrid grouper Epinephelus fuscoguttatus♀ × E. lanceolatus♂ Fishmeal substitute Cottonseed protein concentrate Deep processing |
| title | Effect of replacing fishmeal with 70 cottonseed protein concentrate on growth performance, liver health, muscle texture, intestinal microbiome, and wastewater parameters in hybrid grouper (Epinephelus fuscoguttatus♀ × E. lanceolatus♂) juvenile |
| title_full | Effect of replacing fishmeal with 70 cottonseed protein concentrate on growth performance, liver health, muscle texture, intestinal microbiome, and wastewater parameters in hybrid grouper (Epinephelus fuscoguttatus♀ × E. lanceolatus♂) juvenile |
| title_fullStr | Effect of replacing fishmeal with 70 cottonseed protein concentrate on growth performance, liver health, muscle texture, intestinal microbiome, and wastewater parameters in hybrid grouper (Epinephelus fuscoguttatus♀ × E. lanceolatus♂) juvenile |
| title_full_unstemmed | Effect of replacing fishmeal with 70 cottonseed protein concentrate on growth performance, liver health, muscle texture, intestinal microbiome, and wastewater parameters in hybrid grouper (Epinephelus fuscoguttatus♀ × E. lanceolatus♂) juvenile |
| title_short | Effect of replacing fishmeal with 70 cottonseed protein concentrate on growth performance, liver health, muscle texture, intestinal microbiome, and wastewater parameters in hybrid grouper (Epinephelus fuscoguttatus♀ × E. lanceolatus♂) juvenile |
| title_sort | effect of replacing fishmeal with 70 cottonseed protein concentrate on growth performance liver health muscle texture intestinal microbiome and wastewater parameters in hybrid grouper epinephelus fuscoguttatus♀ e lanceolatus♂ juvenile |
| topic | Hybrid grouper Epinephelus fuscoguttatus♀ × E. lanceolatus♂ Fishmeal substitute Cottonseed protein concentrate Deep processing |
| url | http://www.sciencedirect.com/science/article/pii/S2352513425003230 |
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