Does DNA extraction affect the specificity of a PCR method claiming the specific detectability of a genome-edited plant?
Under current EU legislation, genetically modified organisms (GMOs) and derived food and feed products must be authorized as GM food, feed, or seed and appropriate detection methods must be made available for use in official controls. A Real-Time PCR method has recently been published by Chhalliyil...
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| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2024-12-01
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| Series: | GM Crops & Food |
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| Online Access: | https://www.tandfonline.com/doi/10.1080/21645698.2024.2423441 |
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| _version_ | 1846119881428172800 |
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| author | Sophia Edelmann Christian Savini Dominik Moor Jörn Lämke Kathrin Lieske Marco Mazzara Hendrik Emons Joachim Mankertz Christopher Weidner |
| author_facet | Sophia Edelmann Christian Savini Dominik Moor Jörn Lämke Kathrin Lieske Marco Mazzara Hendrik Emons Joachim Mankertz Christopher Weidner |
| author_sort | Sophia Edelmann |
| collection | DOAJ |
| description | Under current EU legislation, genetically modified organisms (GMOs) and derived food and feed products must be authorized as GM food, feed, or seed and appropriate detection methods must be made available for use in official controls. A Real-Time PCR method has recently been published by Chhalliyil et al. claiming to be specific for the detection and identification of genome-edited oilseed rape (OSR) lines commercialized in North America. In a previous study, we have independently assessed this method in three reference laboratories for sensitivity, specificity, and robustness. We found that the method does not meet all the minimum performance requirements (MPR) for GMO testing in the EU, which contradicts the claims of the method developer. Here we show, in addition to the previously published method assessment study that a modified DNA extraction is not the reason for the contradictory findings and does not affect the specificity of the method. We also discuss the procedures recently proposed by the method developers for interpreting PCR results with high Cq values. |
| format | Article |
| id | doaj-art-f7e3f398026e4ba8b1c95722c92c3e10 |
| institution | Kabale University |
| issn | 2164-5698 2164-5701 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | GM Crops & Food |
| spelling | doaj-art-f7e3f398026e4ba8b1c95722c92c3e102024-12-16T16:41:27ZengTaylor & Francis GroupGM Crops & Food2164-56982164-57012024-12-0115135236010.1080/21645698.2024.2423441Does DNA extraction affect the specificity of a PCR method claiming the specific detectability of a genome-edited plant?Sophia Edelmann0Christian Savini1Dominik Moor2Jörn Lämke3Kathrin Lieske4Marco Mazzara5Hendrik Emons6Joachim Mankertz7Christopher Weidner8Department Method Standardisation, Reference Laboratories, Resistance to Antibiotics, Federal Office of Consumer Protection and Food Safety (BVL), Berlin, GermanyEuropean Commission, Joint Research Centre (JRC), Ispra, ItalyRisk Assessment Division, Federal Food Safety and Veterinary Office (FSVO), Bern, SwitzerlandDepartment Method Standardisation, Reference Laboratories, Resistance to Antibiotics, Federal Office of Consumer Protection and Food Safety (BVL), Berlin, GermanyDepartment Method Standardisation, Reference Laboratories, Resistance to Antibiotics, Federal Office of Consumer Protection and Food Safety (BVL), Berlin, GermanyEuropean Commission, Joint Research Centre (JRC), Ispra, ItalyEuropean Commission, Joint Research Centre (JRC), Geel, BelgiumDepartment Method Standardisation, Reference Laboratories, Resistance to Antibiotics, Federal Office of Consumer Protection and Food Safety (BVL), Berlin, GermanyDepartment Method Standardisation, Reference Laboratories, Resistance to Antibiotics, Federal Office of Consumer Protection and Food Safety (BVL), Berlin, GermanyUnder current EU legislation, genetically modified organisms (GMOs) and derived food and feed products must be authorized as GM food, feed, or seed and appropriate detection methods must be made available for use in official controls. A Real-Time PCR method has recently been published by Chhalliyil et al. claiming to be specific for the detection and identification of genome-edited oilseed rape (OSR) lines commercialized in North America. In a previous study, we have independently assessed this method in three reference laboratories for sensitivity, specificity, and robustness. We found that the method does not meet all the minimum performance requirements (MPR) for GMO testing in the EU, which contradicts the claims of the method developer. Here we show, in addition to the previously published method assessment study that a modified DNA extraction is not the reason for the contradictory findings and does not affect the specificity of the method. We also discuss the procedures recently proposed by the method developers for interpreting PCR results with high Cq values.https://www.tandfonline.com/doi/10.1080/21645698.2024.2423441DNA extractiongenome editingGMO detectionofficial controloilseed rape (OSR) |
| spellingShingle | Sophia Edelmann Christian Savini Dominik Moor Jörn Lämke Kathrin Lieske Marco Mazzara Hendrik Emons Joachim Mankertz Christopher Weidner Does DNA extraction affect the specificity of a PCR method claiming the specific detectability of a genome-edited plant? GM Crops & Food DNA extraction genome editing GMO detection official control oilseed rape (OSR) |
| title | Does DNA extraction affect the specificity of a PCR method claiming the specific detectability of a genome-edited plant? |
| title_full | Does DNA extraction affect the specificity of a PCR method claiming the specific detectability of a genome-edited plant? |
| title_fullStr | Does DNA extraction affect the specificity of a PCR method claiming the specific detectability of a genome-edited plant? |
| title_full_unstemmed | Does DNA extraction affect the specificity of a PCR method claiming the specific detectability of a genome-edited plant? |
| title_short | Does DNA extraction affect the specificity of a PCR method claiming the specific detectability of a genome-edited plant? |
| title_sort | does dna extraction affect the specificity of a pcr method claiming the specific detectability of a genome edited plant |
| topic | DNA extraction genome editing GMO detection official control oilseed rape (OSR) |
| url | https://www.tandfonline.com/doi/10.1080/21645698.2024.2423441 |
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