Plasma Epstein-Barr Virus DNA load for diagnostic and prognostic assessment in intestinal Epstein-Barr Virus infection
BackgroundThe prospective application of plasma Epstein-Barr virus (EBV) DNA load as a noninvasive measure of intestinal EBV infection remains unexplored. This study aims to identify ideal threshold levels for plasma EBV DNA loads in the diagnosis and outcome prediction of intestinal EBV infection,...
Saved in:
| Main Authors: | , , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Frontiers Media S.A.
2025-01-01
|
| Series: | Frontiers in Cellular and Infection Microbiology |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/articles/10.3389/fcimb.2024.1526633/full |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1841556687762227200 |
|---|---|
| author | Chunxiang Ma Chunxiang Ma Chunxiang Ma Mingshan Jiang Mingshan Jiang Mingshan Jiang Jiaxin Li Jiaxin Li Jiaxin Li Zhen Zeng Zhen Zeng Zhen Zeng Yushan Wu Yushan Wu Yushan Wu Rui Cheng Rui Cheng Rui Cheng Hao Lin Hao Lin Hao Lin Jiangmei Pang Jiangmei Pang Jiangmei Pang Fang Yin Fang Yin Fang Yin Yongbin Jia Yongbin Jia Yongbin Jia Lili Li Lili Li Lili Li Hu Zhang Hu Zhang Hu Zhang |
| author_facet | Chunxiang Ma Chunxiang Ma Chunxiang Ma Mingshan Jiang Mingshan Jiang Mingshan Jiang Jiaxin Li Jiaxin Li Jiaxin Li Zhen Zeng Zhen Zeng Zhen Zeng Yushan Wu Yushan Wu Yushan Wu Rui Cheng Rui Cheng Rui Cheng Hao Lin Hao Lin Hao Lin Jiangmei Pang Jiangmei Pang Jiangmei Pang Fang Yin Fang Yin Fang Yin Yongbin Jia Yongbin Jia Yongbin Jia Lili Li Lili Li Lili Li Hu Zhang Hu Zhang Hu Zhang |
| author_sort | Chunxiang Ma |
| collection | DOAJ |
| description | BackgroundThe prospective application of plasma Epstein-Barr virus (EBV) DNA load as a noninvasive measure of intestinal EBV infection remains unexplored. This study aims to identify ideal threshold levels for plasma EBV DNA loads in the diagnosis and outcome prediction of intestinal EBV infection, particularly in cases of primary intestinal lymphoproliferative diseases and inflammatory bowel disease (IBD).MethodsReceiver operating characteristic (ROC) curves were examined to determine suitable thresholds for plasma EBV DNA load in diagnosing intestinal EBV infection and predicting its prognosis.Results108 patients were retrospectively assigned to the test group, while 56 patients were included in the validation group. Plasma EBV DNA loads were significantly higher in the intestinal EBV infection group compared to the non-intestinal EBV infection group (Median: 2.02 × 102 copies/mL, interquartile range [IQR]: 5.49 × 101-6.34×103 copies/mL versus 4.2×101 copies/mL, IQR: 1.07 ×101-6.08×101 copies/mL; P < 0.0001). Plasma EBV DNA levels at 9.21×101 and 6.77×101 copies/mL proved beneficial for the identification and prognostication in intestinal EBV infection, respectively. Values of 0.82 and 0.71 were yielded by the area under the ROC curve (AUC) in the test cohort, corresponding to sensitivities of 84.38% (95% confidence interval [95%CI]: 68.25%-93.14%) and 87.5% (95%CI: 69%-95.66%), specificities of 83.33% (95%CI: 64.15%-93.32%) and 68.09% (95%CI: 53.83%-79.6%), positive predictive values (PPV) of 87.1% (95%CI: 71.15%-94.87%) and 58.33% (95%CI: 42.2%-72.86%), and positive likelihood ratios (LR+) of 5.06 and 2.74 in the validation cohort, respectively. Furthermore, a plasma EBV DNA load of 5.4×102 copies/mL helped differentiate IBD with intestinal EBV infection from primary intestinal EBV-positive lymphoproliferative disorders (PIEBV+LPDs), achieving an AUC of 0.85 within the test cohort, as well as 85% sensitivity (95%CI: 63.96%-94.76%), 91.67% specificity (95%CI: 64.61%-99.57%), 94.44% PPV (95%CI: 74.24%-99.72%), and an LR+ of 10.2 in the validation cohort.ConclusionsPlasma EBV DNA load demonstrates notable potential in distinguishing between different patient cohorts with intestinal EBV infection, although its sensitivity requires further optimization for clinical application. |
| format | Article |
| id | doaj-art-f7d084f000a34d0da2a1c9189c33d5d4 |
| institution | Kabale University |
| issn | 2235-2988 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Cellular and Infection Microbiology |
| spelling | doaj-art-f7d084f000a34d0da2a1c9189c33d5d42025-01-07T06:46:22ZengFrontiers Media S.A.Frontiers in Cellular and Infection Microbiology2235-29882025-01-011410.3389/fcimb.2024.15266331526633Plasma Epstein-Barr Virus DNA load for diagnostic and prognostic assessment in intestinal Epstein-Barr Virus infectionChunxiang Ma0Chunxiang Ma1Chunxiang Ma2Mingshan Jiang3Mingshan Jiang4Mingshan Jiang5Jiaxin Li6Jiaxin Li7Jiaxin Li8Zhen Zeng9Zhen Zeng10Zhen Zeng11Yushan Wu12Yushan Wu13Yushan Wu14Rui Cheng15Rui Cheng16Rui Cheng17Hao Lin18Hao Lin19Hao Lin20Jiangmei Pang21Jiangmei Pang22Jiangmei Pang23Fang Yin24Fang Yin25Fang Yin26Yongbin Jia27Yongbin Jia28Yongbin Jia29Lili Li30Lili Li31Lili Li32Hu Zhang33Hu Zhang34Hu Zhang35Department of Gastroenterology, West China Hospital, Sichuan University, Chengdu, ChinaCentre for Inflammatory Bowel Disease, West China Hospital, Sichuan University, Chengdu, ChinaLab of Inflammatory Bowel Disease, Frontiers Science Center for Disease-Related Molecular Network, West China Hospital, Sichuan University, Chengdu, ChinaDepartment of Gastroenterology, West China Hospital, Sichuan University, Chengdu, ChinaCentre for Inflammatory Bowel Disease, West China Hospital, Sichuan University, Chengdu, ChinaLab of Inflammatory Bowel Disease, Frontiers Science Center for Disease-Related Molecular Network, West China Hospital, Sichuan University, Chengdu, ChinaDepartment of Gastroenterology, West China Hospital, Sichuan University, Chengdu, ChinaCentre for Inflammatory Bowel Disease, West China Hospital, Sichuan University, Chengdu, ChinaLab of Inflammatory Bowel Disease, Frontiers Science Center for Disease-Related Molecular Network, West China Hospital, Sichuan University, Chengdu, ChinaDepartment of Gastroenterology, West China Hospital, Sichuan University, Chengdu, ChinaCentre for Inflammatory Bowel Disease, West China Hospital, Sichuan University, Chengdu, ChinaLab of Inflammatory Bowel Disease, Frontiers Science Center for Disease-Related Molecular Network, West China Hospital, Sichuan University, Chengdu, ChinaDepartment of Gastroenterology, West China Hospital, Sichuan University, Chengdu, ChinaCentre for Inflammatory Bowel Disease, West China Hospital, Sichuan University, Chengdu, ChinaLab of Inflammatory Bowel Disease, Frontiers Science Center for Disease-Related Molecular Network, West China Hospital, Sichuan University, Chengdu, ChinaDepartment of Gastroenterology, West China Hospital, Sichuan University, Chengdu, ChinaCentre for Inflammatory Bowel Disease, West China Hospital, Sichuan University, Chengdu, ChinaLab of Inflammatory Bowel Disease, Frontiers Science Center for Disease-Related Molecular Network, West China Hospital, Sichuan University, Chengdu, ChinaDepartment of Gastroenterology, West China Hospital, Sichuan University, Chengdu, ChinaCentre for Inflammatory Bowel Disease, West China Hospital, Sichuan University, Chengdu, ChinaLab of Inflammatory Bowel Disease, Frontiers Science Center for Disease-Related Molecular Network, West China Hospital, Sichuan University, Chengdu, ChinaDepartment of Gastroenterology, West China Hospital, Sichuan University, Chengdu, ChinaCentre for Inflammatory Bowel Disease, West China Hospital, Sichuan University, Chengdu, ChinaLab of Inflammatory Bowel Disease, Frontiers Science Center for Disease-Related Molecular Network, West China Hospital, Sichuan University, Chengdu, ChinaDepartment of Gastroenterology, West China Hospital, Sichuan University, Chengdu, ChinaCentre for Inflammatory Bowel Disease, West China Hospital, Sichuan University, Chengdu, ChinaLab of Inflammatory Bowel Disease, Frontiers Science Center for Disease-Related Molecular Network, West China Hospital, Sichuan University, Chengdu, ChinaDepartment of Gastroenterology, West China Hospital, Sichuan University, Chengdu, ChinaCentre for Inflammatory Bowel Disease, West China Hospital, Sichuan University, Chengdu, ChinaLab of Inflammatory Bowel Disease, Frontiers Science Center for Disease-Related Molecular Network, West China Hospital, Sichuan University, Chengdu, ChinaDepartment of Gastroenterology, West China Hospital, Sichuan University, Chengdu, ChinaCentre for Inflammatory Bowel Disease, West China Hospital, Sichuan University, Chengdu, ChinaLab of Inflammatory Bowel Disease, Frontiers Science Center for Disease-Related Molecular Network, West China Hospital, Sichuan University, Chengdu, ChinaDepartment of Gastroenterology, West China Hospital, Sichuan University, Chengdu, ChinaCentre for Inflammatory Bowel Disease, West China Hospital, Sichuan University, Chengdu, ChinaLab of Inflammatory Bowel Disease, Frontiers Science Center for Disease-Related Molecular Network, West China Hospital, Sichuan University, Chengdu, ChinaBackgroundThe prospective application of plasma Epstein-Barr virus (EBV) DNA load as a noninvasive measure of intestinal EBV infection remains unexplored. This study aims to identify ideal threshold levels for plasma EBV DNA loads in the diagnosis and outcome prediction of intestinal EBV infection, particularly in cases of primary intestinal lymphoproliferative diseases and inflammatory bowel disease (IBD).MethodsReceiver operating characteristic (ROC) curves were examined to determine suitable thresholds for plasma EBV DNA load in diagnosing intestinal EBV infection and predicting its prognosis.Results108 patients were retrospectively assigned to the test group, while 56 patients were included in the validation group. Plasma EBV DNA loads were significantly higher in the intestinal EBV infection group compared to the non-intestinal EBV infection group (Median: 2.02 × 102 copies/mL, interquartile range [IQR]: 5.49 × 101-6.34×103 copies/mL versus 4.2×101 copies/mL, IQR: 1.07 ×101-6.08×101 copies/mL; P < 0.0001). Plasma EBV DNA levels at 9.21×101 and 6.77×101 copies/mL proved beneficial for the identification and prognostication in intestinal EBV infection, respectively. Values of 0.82 and 0.71 were yielded by the area under the ROC curve (AUC) in the test cohort, corresponding to sensitivities of 84.38% (95% confidence interval [95%CI]: 68.25%-93.14%) and 87.5% (95%CI: 69%-95.66%), specificities of 83.33% (95%CI: 64.15%-93.32%) and 68.09% (95%CI: 53.83%-79.6%), positive predictive values (PPV) of 87.1% (95%CI: 71.15%-94.87%) and 58.33% (95%CI: 42.2%-72.86%), and positive likelihood ratios (LR+) of 5.06 and 2.74 in the validation cohort, respectively. Furthermore, a plasma EBV DNA load of 5.4×102 copies/mL helped differentiate IBD with intestinal EBV infection from primary intestinal EBV-positive lymphoproliferative disorders (PIEBV+LPDs), achieving an AUC of 0.85 within the test cohort, as well as 85% sensitivity (95%CI: 63.96%-94.76%), 91.67% specificity (95%CI: 64.61%-99.57%), 94.44% PPV (95%CI: 74.24%-99.72%), and an LR+ of 10.2 in the validation cohort.ConclusionsPlasma EBV DNA load demonstrates notable potential in distinguishing between different patient cohorts with intestinal EBV infection, although its sensitivity requires further optimization for clinical application.https://www.frontiersin.org/articles/10.3389/fcimb.2024.1526633/fullEpstein-Barr virus DNA loaddiagnosisprognosisinflammatory bowel diseasesprimary intestinal lymphoproliferative diseasesintestinal Epstein-Barr Virus infection |
| spellingShingle | Chunxiang Ma Chunxiang Ma Chunxiang Ma Mingshan Jiang Mingshan Jiang Mingshan Jiang Jiaxin Li Jiaxin Li Jiaxin Li Zhen Zeng Zhen Zeng Zhen Zeng Yushan Wu Yushan Wu Yushan Wu Rui Cheng Rui Cheng Rui Cheng Hao Lin Hao Lin Hao Lin Jiangmei Pang Jiangmei Pang Jiangmei Pang Fang Yin Fang Yin Fang Yin Yongbin Jia Yongbin Jia Yongbin Jia Lili Li Lili Li Lili Li Hu Zhang Hu Zhang Hu Zhang Plasma Epstein-Barr Virus DNA load for diagnostic and prognostic assessment in intestinal Epstein-Barr Virus infection Frontiers in Cellular and Infection Microbiology Epstein-Barr virus DNA load diagnosis prognosis inflammatory bowel diseases primary intestinal lymphoproliferative diseases intestinal Epstein-Barr Virus infection |
| title | Plasma Epstein-Barr Virus DNA load for diagnostic and prognostic assessment in intestinal Epstein-Barr Virus infection |
| title_full | Plasma Epstein-Barr Virus DNA load for diagnostic and prognostic assessment in intestinal Epstein-Barr Virus infection |
| title_fullStr | Plasma Epstein-Barr Virus DNA load for diagnostic and prognostic assessment in intestinal Epstein-Barr Virus infection |
| title_full_unstemmed | Plasma Epstein-Barr Virus DNA load for diagnostic and prognostic assessment in intestinal Epstein-Barr Virus infection |
| title_short | Plasma Epstein-Barr Virus DNA load for diagnostic and prognostic assessment in intestinal Epstein-Barr Virus infection |
| title_sort | plasma epstein barr virus dna load for diagnostic and prognostic assessment in intestinal epstein barr virus infection |
| topic | Epstein-Barr virus DNA load diagnosis prognosis inflammatory bowel diseases primary intestinal lymphoproliferative diseases intestinal Epstein-Barr Virus infection |
| url | https://www.frontiersin.org/articles/10.3389/fcimb.2024.1526633/full |
| work_keys_str_mv | AT chunxiangma plasmaepsteinbarrvirusdnaloadfordiagnosticandprognosticassessmentinintestinalepsteinbarrvirusinfection AT chunxiangma plasmaepsteinbarrvirusdnaloadfordiagnosticandprognosticassessmentinintestinalepsteinbarrvirusinfection AT chunxiangma plasmaepsteinbarrvirusdnaloadfordiagnosticandprognosticassessmentinintestinalepsteinbarrvirusinfection AT mingshanjiang plasmaepsteinbarrvirusdnaloadfordiagnosticandprognosticassessmentinintestinalepsteinbarrvirusinfection AT mingshanjiang plasmaepsteinbarrvirusdnaloadfordiagnosticandprognosticassessmentinintestinalepsteinbarrvirusinfection AT mingshanjiang plasmaepsteinbarrvirusdnaloadfordiagnosticandprognosticassessmentinintestinalepsteinbarrvirusinfection AT jiaxinli plasmaepsteinbarrvirusdnaloadfordiagnosticandprognosticassessmentinintestinalepsteinbarrvirusinfection AT jiaxinli plasmaepsteinbarrvirusdnaloadfordiagnosticandprognosticassessmentinintestinalepsteinbarrvirusinfection AT jiaxinli plasmaepsteinbarrvirusdnaloadfordiagnosticandprognosticassessmentinintestinalepsteinbarrvirusinfection AT zhenzeng plasmaepsteinbarrvirusdnaloadfordiagnosticandprognosticassessmentinintestinalepsteinbarrvirusinfection AT zhenzeng plasmaepsteinbarrvirusdnaloadfordiagnosticandprognosticassessmentinintestinalepsteinbarrvirusinfection AT zhenzeng plasmaepsteinbarrvirusdnaloadfordiagnosticandprognosticassessmentinintestinalepsteinbarrvirusinfection AT yushanwu plasmaepsteinbarrvirusdnaloadfordiagnosticandprognosticassessmentinintestinalepsteinbarrvirusinfection AT yushanwu plasmaepsteinbarrvirusdnaloadfordiagnosticandprognosticassessmentinintestinalepsteinbarrvirusinfection AT yushanwu plasmaepsteinbarrvirusdnaloadfordiagnosticandprognosticassessmentinintestinalepsteinbarrvirusinfection AT ruicheng plasmaepsteinbarrvirusdnaloadfordiagnosticandprognosticassessmentinintestinalepsteinbarrvirusinfection AT ruicheng plasmaepsteinbarrvirusdnaloadfordiagnosticandprognosticassessmentinintestinalepsteinbarrvirusinfection AT ruicheng plasmaepsteinbarrvirusdnaloadfordiagnosticandprognosticassessmentinintestinalepsteinbarrvirusinfection AT haolin plasmaepsteinbarrvirusdnaloadfordiagnosticandprognosticassessmentinintestinalepsteinbarrvirusinfection AT haolin plasmaepsteinbarrvirusdnaloadfordiagnosticandprognosticassessmentinintestinalepsteinbarrvirusinfection AT haolin plasmaepsteinbarrvirusdnaloadfordiagnosticandprognosticassessmentinintestinalepsteinbarrvirusinfection AT jiangmeipang plasmaepsteinbarrvirusdnaloadfordiagnosticandprognosticassessmentinintestinalepsteinbarrvirusinfection AT jiangmeipang plasmaepsteinbarrvirusdnaloadfordiagnosticandprognosticassessmentinintestinalepsteinbarrvirusinfection AT jiangmeipang plasmaepsteinbarrvirusdnaloadfordiagnosticandprognosticassessmentinintestinalepsteinbarrvirusinfection AT fangyin plasmaepsteinbarrvirusdnaloadfordiagnosticandprognosticassessmentinintestinalepsteinbarrvirusinfection AT fangyin plasmaepsteinbarrvirusdnaloadfordiagnosticandprognosticassessmentinintestinalepsteinbarrvirusinfection AT fangyin plasmaepsteinbarrvirusdnaloadfordiagnosticandprognosticassessmentinintestinalepsteinbarrvirusinfection AT yongbinjia plasmaepsteinbarrvirusdnaloadfordiagnosticandprognosticassessmentinintestinalepsteinbarrvirusinfection AT yongbinjia plasmaepsteinbarrvirusdnaloadfordiagnosticandprognosticassessmentinintestinalepsteinbarrvirusinfection AT yongbinjia plasmaepsteinbarrvirusdnaloadfordiagnosticandprognosticassessmentinintestinalepsteinbarrvirusinfection AT lilili plasmaepsteinbarrvirusdnaloadfordiagnosticandprognosticassessmentinintestinalepsteinbarrvirusinfection AT lilili plasmaepsteinbarrvirusdnaloadfordiagnosticandprognosticassessmentinintestinalepsteinbarrvirusinfection AT lilili plasmaepsteinbarrvirusdnaloadfordiagnosticandprognosticassessmentinintestinalepsteinbarrvirusinfection AT huzhang plasmaepsteinbarrvirusdnaloadfordiagnosticandprognosticassessmentinintestinalepsteinbarrvirusinfection AT huzhang plasmaepsteinbarrvirusdnaloadfordiagnosticandprognosticassessmentinintestinalepsteinbarrvirusinfection AT huzhang plasmaepsteinbarrvirusdnaloadfordiagnosticandprognosticassessmentinintestinalepsteinbarrvirusinfection |