Protocol for 3D bioprinting of nanoparticle-laden hydrogels to enhance antibacterial and imaging properties
Summary: This protocol describes the preparation of a nanoparticle-encapsulated bioink capable of protecting tissue-engineered constructs against bacterial infections while also providing contrast for magnetic resonance (MR) imaging modalities. The report includes details of preparing the methacryla...
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| Format: | Article |
| Language: | English |
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Elsevier
2024-12-01
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| Series: | STAR Protocols |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2666166724005586 |
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| author | Mehdi Salar Amoli Linqi Jin Sarah Rezapourdamanab Maher Saadeh Yamini Singh Liqun Ning Boeun Hwang Martin L. Tomov Christopher N. LaRock Morteza Mahmoudi Holly Bauser-Heaton Vahid Serpooshan |
| author_facet | Mehdi Salar Amoli Linqi Jin Sarah Rezapourdamanab Maher Saadeh Yamini Singh Liqun Ning Boeun Hwang Martin L. Tomov Christopher N. LaRock Morteza Mahmoudi Holly Bauser-Heaton Vahid Serpooshan |
| author_sort | Mehdi Salar Amoli |
| collection | DOAJ |
| description | Summary: This protocol describes the preparation of a nanoparticle-encapsulated bioink capable of protecting tissue-engineered constructs against bacterial infections while also providing contrast for magnetic resonance (MR) imaging modalities. The report includes details of preparing the methacrylated gelatin-based bioinks and the incorporation of superparamagnetic iron oxide nanoparticles. A detailed protocol is presented for characterizing the bioink, evaluating cell response, and assessing its antibacterial effect. Overall, this article presents a robust approach for the development of antibacterial, MR-visible bioinks suitable for various tissue engineering applications.For complete details on the use and execution of this protocol, please refer to Theus et al.1 : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics. |
| format | Article |
| id | doaj-art-f7c78c8e180a497e96ed448a8186e33a |
| institution | Kabale University |
| issn | 2666-1667 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | Elsevier |
| record_format | Article |
| series | STAR Protocols |
| spelling | doaj-art-f7c78c8e180a497e96ed448a8186e33a2024-11-20T05:08:03ZengElsevierSTAR Protocols2666-16672024-12-0154103393Protocol for 3D bioprinting of nanoparticle-laden hydrogels to enhance antibacterial and imaging propertiesMehdi Salar Amoli0Linqi Jin1Sarah Rezapourdamanab2Maher Saadeh3Yamini Singh4Liqun Ning5Boeun Hwang6Martin L. Tomov7Christopher N. LaRock8Morteza Mahmoudi9Holly Bauser-Heaton10Vahid Serpooshan11Department of Biomedical Engineering, Emory University School of Medicine and Georgia Institute of Technology, Atlanta, GA 30322, USA; Corresponding authorDepartment of Biomedical Engineering, Emory University School of Medicine and Georgia Institute of Technology, Atlanta, GA 30322, USADepartment of Biomedical Engineering, Emory University School of Medicine and Georgia Institute of Technology, Atlanta, GA 30322, USADepartment of Biomedical Engineering, Emory University School of Medicine and Georgia Institute of Technology, Atlanta, GA 30322, USADepartment of Biomedical Engineering, Emory University School of Medicine and Georgia Institute of Technology, Atlanta, GA 30322, USADepartment of Biomedical Engineering, Emory University School of Medicine and Georgia Institute of Technology, Atlanta, GA 30322, USA; Department of Mechanical Engineering, Cleveland State University, Cleveland, OH 44115, USADepartment of Biomedical Engineering, Emory University School of Medicine and Georgia Institute of Technology, Atlanta, GA 30322, USADepartment of Biomedical Engineering, Emory University School of Medicine and Georgia Institute of Technology, Atlanta, GA 30322, USA; Department of Pediatrics, Emory University School of Medicine, Atlanta, GA 30322, USADepartment of Microbiology and Immunology, Emory University School of Medicine, Atlanta, GA, USA; Emory Antibiotic Resistance Center, Emory University School of Medicine, Atlanta, GA, USA; Division of Infectious Diseases, Department of Medicine, Emory University School of Medicine, Atlanta, GA, USAPrecision Health Program, Michigan State University, East Lansing, MI, USADepartment of Biomedical Engineering, Emory University School of Medicine and Georgia Institute of Technology, Atlanta, GA 30322, USA; Department of Pediatrics, Emory University School of Medicine, Atlanta, GA 30322, USA; Children’s Healthcare of Atlanta, Atlanta, GA 30322, USA; Sibley Heart Center at Children’s Healthcare of Atlanta, Atlanta, GA, USADepartment of Biomedical Engineering, Emory University School of Medicine and Georgia Institute of Technology, Atlanta, GA 30322, USA; Department of Pediatrics, Emory University School of Medicine, Atlanta, GA 30322, USA; Children’s Healthcare of Atlanta, Atlanta, GA 30322, USA; Corresponding authorSummary: This protocol describes the preparation of a nanoparticle-encapsulated bioink capable of protecting tissue-engineered constructs against bacterial infections while also providing contrast for magnetic resonance (MR) imaging modalities. The report includes details of preparing the methacrylated gelatin-based bioinks and the incorporation of superparamagnetic iron oxide nanoparticles. A detailed protocol is presented for characterizing the bioink, evaluating cell response, and assessing its antibacterial effect. Overall, this article presents a robust approach for the development of antibacterial, MR-visible bioinks suitable for various tissue engineering applications.For complete details on the use and execution of this protocol, please refer to Theus et al.1 : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics.http://www.sciencedirect.com/science/article/pii/S2666166724005586tissue engineeringbiotechnology and bioengineering |
| spellingShingle | Mehdi Salar Amoli Linqi Jin Sarah Rezapourdamanab Maher Saadeh Yamini Singh Liqun Ning Boeun Hwang Martin L. Tomov Christopher N. LaRock Morteza Mahmoudi Holly Bauser-Heaton Vahid Serpooshan Protocol for 3D bioprinting of nanoparticle-laden hydrogels to enhance antibacterial and imaging properties STAR Protocols tissue engineering biotechnology and bioengineering |
| title | Protocol for 3D bioprinting of nanoparticle-laden hydrogels to enhance antibacterial and imaging properties |
| title_full | Protocol for 3D bioprinting of nanoparticle-laden hydrogels to enhance antibacterial and imaging properties |
| title_fullStr | Protocol for 3D bioprinting of nanoparticle-laden hydrogels to enhance antibacterial and imaging properties |
| title_full_unstemmed | Protocol for 3D bioprinting of nanoparticle-laden hydrogels to enhance antibacterial and imaging properties |
| title_short | Protocol for 3D bioprinting of nanoparticle-laden hydrogels to enhance antibacterial and imaging properties |
| title_sort | protocol for 3d bioprinting of nanoparticle laden hydrogels to enhance antibacterial and imaging properties |
| topic | tissue engineering biotechnology and bioengineering |
| url | http://www.sciencedirect.com/science/article/pii/S2666166724005586 |
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