Antimicrobial Activity and Mechanism of Action of Litsea cubeba Essential Oil against Cladosporium
In this study, essential oil was extracted from the fruit of Litsea cubeba by hydrodistillation, and its major components were analyzed by gas chromatography-mass spectrometry (GC-MS). The antimicrobial activity of the essential oil against Cladosporium was determined and its mechanism of action was...
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China Food Publishing Company
2025-05-01
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| author | PENG Yuanyuan, LIU Zhenyu, HUANG Qi, XIE Sha, BI Xiufang, HUANG Meigui, HAO Gang |
| author_facet | PENG Yuanyuan, LIU Zhenyu, HUANG Qi, XIE Sha, BI Xiufang, HUANG Meigui, HAO Gang |
| author_sort | PENG Yuanyuan, LIU Zhenyu, HUANG Qi, XIE Sha, BI Xiufang, HUANG Meigui, HAO Gang |
| collection | DOAJ |
| description | In this study, essential oil was extracted from the fruit of Litsea cubeba by hydrodistillation, and its major components were analyzed by gas chromatography-mass spectrometry (GC-MS). The antimicrobial activity of the essential oil against Cladosporium was determined and its mechanism of action was explored by examining the antibacterial rate and mycelium inhibition rate of Cladosporium, the liposome leakage assay, and the determination of leakage of intracellular ions and macromolecular contents and ATPase activity. The results indicated that the major components of L. cubeba essential oil were terpenoids such as citral and monoterpene oxides. Its minimum inhibitory concentration against Cladosporium was 0.64% (V/V). The essential oil was effective in inhibiting the growth of Cladosporium mycelium and altering its molecular structure and functional groups. The initial target for the antimicrobial activity of the essential oil was the cell membrane, where it increased the permeability of the phospholipid bilayer in a concentration-dependent manner, leading to the leakage of fluorescein encapsulated in liposomes without collapsing the bilayer. It also caused the leakage of intracellular K+, Ca2+, and macromolecules, thereby inhibiting the growth and reproduction of Cladosporium. Scanning electron microscope observation further confirmed that L. cubeba essential oil disrupted the morphology of Cladosporium mycelium, resulting in severe loss of intracellular contents. |
| format | Article |
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| institution | Kabale University |
| issn | 1002-6630 |
| language | English |
| publishDate | 2025-05-01 |
| publisher | China Food Publishing Company |
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| series | Shipin Kexue |
| spelling | doaj-art-f7c34e5d873e4f9da8f7a15f672dcb4c2025-08-20T03:25:07ZengChina Food Publishing CompanyShipin Kexue1002-66302025-05-014610515810.7506/spkx1002-6630-20240708-081Antimicrobial Activity and Mechanism of Action of Litsea cubeba Essential Oil against CladosporiumPENG Yuanyuan, LIU Zhenyu, HUANG Qi, XIE Sha, BI Xiufang, HUANG Meigui, HAO Gang0(College of Pharmacy and Food, Southwest Minzu University, Chengdu 610041, China)In this study, essential oil was extracted from the fruit of Litsea cubeba by hydrodistillation, and its major components were analyzed by gas chromatography-mass spectrometry (GC-MS). The antimicrobial activity of the essential oil against Cladosporium was determined and its mechanism of action was explored by examining the antibacterial rate and mycelium inhibition rate of Cladosporium, the liposome leakage assay, and the determination of leakage of intracellular ions and macromolecular contents and ATPase activity. The results indicated that the major components of L. cubeba essential oil were terpenoids such as citral and monoterpene oxides. Its minimum inhibitory concentration against Cladosporium was 0.64% (V/V). The essential oil was effective in inhibiting the growth of Cladosporium mycelium and altering its molecular structure and functional groups. The initial target for the antimicrobial activity of the essential oil was the cell membrane, where it increased the permeability of the phospholipid bilayer in a concentration-dependent manner, leading to the leakage of fluorescein encapsulated in liposomes without collapsing the bilayer. It also caused the leakage of intracellular K+, Ca2+, and macromolecules, thereby inhibiting the growth and reproduction of Cladosporium. Scanning electron microscope observation further confirmed that L. cubeba essential oil disrupted the morphology of Cladosporium mycelium, resulting in severe loss of intracellular contents.https://www.spkx.net.cn/fileup/1002-6630/PDF/2025-46-10-006.pdfcladosporium; litsea cubeba essential oil; antimicrobial activity; antimicrobial mechanism |
| spellingShingle | PENG Yuanyuan, LIU Zhenyu, HUANG Qi, XIE Sha, BI Xiufang, HUANG Meigui, HAO Gang Antimicrobial Activity and Mechanism of Action of Litsea cubeba Essential Oil against Cladosporium Shipin Kexue cladosporium; litsea cubeba essential oil; antimicrobial activity; antimicrobial mechanism |
| title | Antimicrobial Activity and Mechanism of Action of Litsea cubeba Essential Oil against Cladosporium |
| title_full | Antimicrobial Activity and Mechanism of Action of Litsea cubeba Essential Oil against Cladosporium |
| title_fullStr | Antimicrobial Activity and Mechanism of Action of Litsea cubeba Essential Oil against Cladosporium |
| title_full_unstemmed | Antimicrobial Activity and Mechanism of Action of Litsea cubeba Essential Oil against Cladosporium |
| title_short | Antimicrobial Activity and Mechanism of Action of Litsea cubeba Essential Oil against Cladosporium |
| title_sort | antimicrobial activity and mechanism of action of litsea cubeba essential oil against cladosporium |
| topic | cladosporium; litsea cubeba essential oil; antimicrobial activity; antimicrobial mechanism |
| url | https://www.spkx.net.cn/fileup/1002-6630/PDF/2025-46-10-006.pdf |
| work_keys_str_mv | AT pengyuanyuanliuzhenyuhuangqixieshabixiufanghuangmeiguihaogang antimicrobialactivityandmechanismofactionoflitseacubebaessentialoilagainstcladosporium |