Cartilage defect repair in a rat model via a nanocomposite hydrogel loaded with melatonin-loaded gelatin nanofibers and menstrual blood stem cells: an in vitro and in vivo study
Abstract Cartilage damage caused by injuries or degenerative diseases remains a major challenge in the field of regenerative medicine. In this study, we developed a composite hydrogel system for the delivery of melatonin and menstrual blood stem cells (MenSCs) to treat a rat model of cartilage defec...
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| Format: | Article |
| Language: | English |
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Springer
2024-09-01
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| Series: | Journal of Materials Science: Materials in Medicine |
| Online Access: | https://doi.org/10.1007/s10856-024-06820-z |
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| _version_ | 1846112962689892352 |
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| author | Libo Yuan Ling Yao Xianzhen Ren Xusheng Chen Xu Li Yongqing Xu Tao Jin |
| author_facet | Libo Yuan Ling Yao Xianzhen Ren Xusheng Chen Xu Li Yongqing Xu Tao Jin |
| author_sort | Libo Yuan |
| collection | DOAJ |
| description | Abstract Cartilage damage caused by injuries or degenerative diseases remains a major challenge in the field of regenerative medicine. In this study, we developed a composite hydrogel system for the delivery of melatonin and menstrual blood stem cells (MenSCs) to treat a rat model of cartilage defect. The composite delivery system was produced by incorporation of melatonin into the gelatin fibers and dispersing these fibers into calcium alginate hydrogels. Various characterization methods including cell viability assay, microstructure studies, degradation rate measurement, drug release, anti-inflammatory assay, and radical scavenging assay were used to characterize the hydrogel system. MenSCs were encapsulated within the nanocomposite hydrogel and implanted into a rat model of full-thickness cartilage defect. A 1.3 mm diameter drilled in the femoral trochlea and used for the in vivo study. Results showed that the healing potential of nanocomposite hydrogels containing melatonin and MenSCs was significantly higher than polymer-only hydrogels. Our study introduces a novel composite hydrogel system, combining melatonin and MenSCs, demonstrating enhanced cartilage repair efficacy, offering a promising avenue for regenerative medicine. Graphical Abstract |
| format | Article |
| id | doaj-art-f7aaaec129a0494087dfe8b62b9872b3 |
| institution | Kabale University |
| issn | 1573-4838 |
| language | English |
| publishDate | 2024-09-01 |
| publisher | Springer |
| record_format | Article |
| series | Journal of Materials Science: Materials in Medicine |
| spelling | doaj-art-f7aaaec129a0494087dfe8b62b9872b32024-12-22T12:11:16ZengSpringerJournal of Materials Science: Materials in Medicine1573-48382024-09-0135111210.1007/s10856-024-06820-zCartilage defect repair in a rat model via a nanocomposite hydrogel loaded with melatonin-loaded gelatin nanofibers and menstrual blood stem cells: an in vitro and in vivo studyLibo Yuan0Ling Yao1Xianzhen Ren2Xusheng Chen3Xu Li4Yongqing Xu5Tao Jin6Department of Orthopedics, 920 Hospital of Joint Logistic Support ForceDepartment of Orthopedics, 920 Hospital of Joint Logistic Support ForceDepartment of Orthopedics, 920 Hospital of Joint Logistic Support ForceDepartment of Orthopedics, 920 Hospital of Joint Logistic Support ForceDepartment of Orthopedics, 920 Hospital of Joint Logistic Support ForceDepartment of Orthopedics, 920 Hospital of Joint Logistic Support ForceDepartment of Orthopedics, 920 Hospital of Joint Logistic Support ForceAbstract Cartilage damage caused by injuries or degenerative diseases remains a major challenge in the field of regenerative medicine. In this study, we developed a composite hydrogel system for the delivery of melatonin and menstrual blood stem cells (MenSCs) to treat a rat model of cartilage defect. The composite delivery system was produced by incorporation of melatonin into the gelatin fibers and dispersing these fibers into calcium alginate hydrogels. Various characterization methods including cell viability assay, microstructure studies, degradation rate measurement, drug release, anti-inflammatory assay, and radical scavenging assay were used to characterize the hydrogel system. MenSCs were encapsulated within the nanocomposite hydrogel and implanted into a rat model of full-thickness cartilage defect. A 1.3 mm diameter drilled in the femoral trochlea and used for the in vivo study. Results showed that the healing potential of nanocomposite hydrogels containing melatonin and MenSCs was significantly higher than polymer-only hydrogels. Our study introduces a novel composite hydrogel system, combining melatonin and MenSCs, demonstrating enhanced cartilage repair efficacy, offering a promising avenue for regenerative medicine. Graphical Abstracthttps://doi.org/10.1007/s10856-024-06820-z |
| spellingShingle | Libo Yuan Ling Yao Xianzhen Ren Xusheng Chen Xu Li Yongqing Xu Tao Jin Cartilage defect repair in a rat model via a nanocomposite hydrogel loaded with melatonin-loaded gelatin nanofibers and menstrual blood stem cells: an in vitro and in vivo study Journal of Materials Science: Materials in Medicine |
| title | Cartilage defect repair in a rat model via a nanocomposite hydrogel loaded with melatonin-loaded gelatin nanofibers and menstrual blood stem cells: an in vitro and in vivo study |
| title_full | Cartilage defect repair in a rat model via a nanocomposite hydrogel loaded with melatonin-loaded gelatin nanofibers and menstrual blood stem cells: an in vitro and in vivo study |
| title_fullStr | Cartilage defect repair in a rat model via a nanocomposite hydrogel loaded with melatonin-loaded gelatin nanofibers and menstrual blood stem cells: an in vitro and in vivo study |
| title_full_unstemmed | Cartilage defect repair in a rat model via a nanocomposite hydrogel loaded with melatonin-loaded gelatin nanofibers and menstrual blood stem cells: an in vitro and in vivo study |
| title_short | Cartilage defect repair in a rat model via a nanocomposite hydrogel loaded with melatonin-loaded gelatin nanofibers and menstrual blood stem cells: an in vitro and in vivo study |
| title_sort | cartilage defect repair in a rat model via a nanocomposite hydrogel loaded with melatonin loaded gelatin nanofibers and menstrual blood stem cells an in vitro and in vivo study |
| url | https://doi.org/10.1007/s10856-024-06820-z |
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