Probe-Based Confocal Laser Endomicroscopy Evaluation of Colon Preneoplastic Lesions, with Particular Attention to the Aberrant Crypt Foci, and Comparative Assessment with Histological Features Obtained by Conventional Endoscopy
The colorectal carcinoma represents one of the most common and aggressive malignancies, still characterized by an unacceptable mortality rate, mainly due to the high metastatic potential and to a late diagnosis. In the last years, the research community focused on the chance of improving the endosco...
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| Format: | Article |
| Language: | English |
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Wiley
2012-01-01
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| Series: | Gastroenterology Research and Practice |
| Online Access: | http://dx.doi.org/10.1155/2012/645173 |
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| author | Massimo Mascolo Stefania Staibano Gennaro Ilardi Maria Siano Maria Luisa Vecchione Dario Esposito Gaetano De Rosa Giovanni Domenico De Palma |
| author_facet | Massimo Mascolo Stefania Staibano Gennaro Ilardi Maria Siano Maria Luisa Vecchione Dario Esposito Gaetano De Rosa Giovanni Domenico De Palma |
| author_sort | Massimo Mascolo |
| collection | DOAJ |
| description | The colorectal carcinoma represents one of the most common and aggressive malignancies, still characterized by an unacceptable mortality rate, mainly due to the high metastatic potential and to a late diagnosis. In the last years, the research community focused on the chance of improving the endoscopic screening to detect neoplastic lesions in a very early stage. Several studies proposed aberrant colonic crypt foci as the earliest recognizable step of transformation in colonic multiphase carcinogenesis. We previously demonstrated the clinical applicability and predictive power of probe-based confocal laser endoscopy (pCLE) in superficial colorectal neoplastic lesions and also characterized in vivo a case of dysplasia-associated lesional mass (DALM) in ulcerative colitis. Now, we aim to evaluate the accuracy of pCLE in the detection of ACF comparing in double-blind manner the microendoscopic and histopathological features resulting from colonic biopsy. By pCLE, we identified specific crypt architecture modifications associated with changes in cellular infiltration and vessels architecture, highlighting a good correspondence between pCLE features and histology. |
| format | Article |
| id | doaj-art-f79b4b18a00341d59d50fcc7113730b0 |
| institution | Kabale University |
| issn | 1687-6121 1687-630X |
| language | English |
| publishDate | 2012-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Gastroenterology Research and Practice |
| spelling | doaj-art-f79b4b18a00341d59d50fcc7113730b02025-08-20T03:25:38ZengWileyGastroenterology Research and Practice1687-61211687-630X2012-01-01201210.1155/2012/645173645173Probe-Based Confocal Laser Endomicroscopy Evaluation of Colon Preneoplastic Lesions, with Particular Attention to the Aberrant Crypt Foci, and Comparative Assessment with Histological Features Obtained by Conventional EndoscopyMassimo Mascolo0Stefania Staibano1Gennaro Ilardi2Maria Siano3Maria Luisa Vecchione4Dario Esposito5Gaetano De Rosa6Giovanni Domenico De Palma7Department of Biomorphological and Functional Sciences, Pathology Section, University of Naples “Federico II”, 80131 Naples, ItalyDepartment of Biomorphological and Functional Sciences, Pathology Section, University of Naples “Federico II”, 80131 Naples, ItalyDepartment of Biomorphological and Functional Sciences, Pathology Section, University of Naples “Federico II”, 80131 Naples, ItalyDepartment of Biomorphological and Functional Sciences, Pathology Section, University of Naples “Federico II”, 80131 Naples, ItalyDepartment of Biomorphological and Functional Sciences, Pathology Section, University of Naples “Federico II”, 80131 Naples, ItalyCenter of Excellence for Technical Innovation in Surgery (ITC), Department of General Surgery, Geriatrics, Oncology, and Advanced Technology, University of Naples “Federico II”, 80131 Naples, ItalyDepartment of Biomorphological and Functional Sciences, Pathology Section, University of Naples “Federico II”, 80131 Naples, ItalyCenter of Excellence for Technical Innovation in Surgery (ITC), Department of General Surgery, Geriatrics, Oncology, and Advanced Technology, University of Naples “Federico II”, 80131 Naples, ItalyThe colorectal carcinoma represents one of the most common and aggressive malignancies, still characterized by an unacceptable mortality rate, mainly due to the high metastatic potential and to a late diagnosis. In the last years, the research community focused on the chance of improving the endoscopic screening to detect neoplastic lesions in a very early stage. Several studies proposed aberrant colonic crypt foci as the earliest recognizable step of transformation in colonic multiphase carcinogenesis. We previously demonstrated the clinical applicability and predictive power of probe-based confocal laser endoscopy (pCLE) in superficial colorectal neoplastic lesions and also characterized in vivo a case of dysplasia-associated lesional mass (DALM) in ulcerative colitis. Now, we aim to evaluate the accuracy of pCLE in the detection of ACF comparing in double-blind manner the microendoscopic and histopathological features resulting from colonic biopsy. By pCLE, we identified specific crypt architecture modifications associated with changes in cellular infiltration and vessels architecture, highlighting a good correspondence between pCLE features and histology.http://dx.doi.org/10.1155/2012/645173 |
| spellingShingle | Massimo Mascolo Stefania Staibano Gennaro Ilardi Maria Siano Maria Luisa Vecchione Dario Esposito Gaetano De Rosa Giovanni Domenico De Palma Probe-Based Confocal Laser Endomicroscopy Evaluation of Colon Preneoplastic Lesions, with Particular Attention to the Aberrant Crypt Foci, and Comparative Assessment with Histological Features Obtained by Conventional Endoscopy Gastroenterology Research and Practice |
| title | Probe-Based Confocal Laser Endomicroscopy Evaluation of Colon Preneoplastic Lesions, with Particular Attention to the Aberrant Crypt Foci, and Comparative Assessment with Histological Features Obtained by Conventional Endoscopy |
| title_full | Probe-Based Confocal Laser Endomicroscopy Evaluation of Colon Preneoplastic Lesions, with Particular Attention to the Aberrant Crypt Foci, and Comparative Assessment with Histological Features Obtained by Conventional Endoscopy |
| title_fullStr | Probe-Based Confocal Laser Endomicroscopy Evaluation of Colon Preneoplastic Lesions, with Particular Attention to the Aberrant Crypt Foci, and Comparative Assessment with Histological Features Obtained by Conventional Endoscopy |
| title_full_unstemmed | Probe-Based Confocal Laser Endomicroscopy Evaluation of Colon Preneoplastic Lesions, with Particular Attention to the Aberrant Crypt Foci, and Comparative Assessment with Histological Features Obtained by Conventional Endoscopy |
| title_short | Probe-Based Confocal Laser Endomicroscopy Evaluation of Colon Preneoplastic Lesions, with Particular Attention to the Aberrant Crypt Foci, and Comparative Assessment with Histological Features Obtained by Conventional Endoscopy |
| title_sort | probe based confocal laser endomicroscopy evaluation of colon preneoplastic lesions with particular attention to the aberrant crypt foci and comparative assessment with histological features obtained by conventional endoscopy |
| url | http://dx.doi.org/10.1155/2012/645173 |
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