Ellagic acid could prevent experimentally induced oxidative stress in rats
<b>Aim:</b> Reactive oxygen species (ROS) are produced during the metabolism of diethylnitrosamine (DEN) and phenobarbital (PB). Ellagic acid (EA) is a natural biologically active polyphenol with antioxidant activity. The present study was conducted to assess the preventive and protectiv...
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| Format: | Article |
| Language: | English |
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Selcuk University Press
2022-12-01
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| Series: | Eurasian Journal of Veterinary Sciences |
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| Online Access: | http://eurasianjvetsci.org/pdf.php3?id=1407 |
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| author | Mehmet Ali Kisacam Gonca Ozan Kocamuftuoglu Enver Ozan Sema Temizer Ozan |
| author_facet | Mehmet Ali Kisacam Gonca Ozan Kocamuftuoglu Enver Ozan Sema Temizer Ozan |
| author_sort | Mehmet Ali Kisacam |
| collection | DOAJ |
| description | <b>Aim:</b> Reactive oxygen species (ROS) are produced during the metabolism
of diethylnitrosamine (DEN) and phenobarbital (PB). Ellagic acid (EA) is a
natural biologically active polyphenol with antioxidant activity. The present
study was conducted to assess the preventive and protective capability of EA
on DEN-PB-induced oxidative damage in rats.<p>
<b>Materials and Methods:</b> 60 Sprague-Dawley rats were assigned into 6 groups:
Negative control, DEN, EA, DEN-PB (positive control), DEN-PB-EA (treatment),
and EA-DEN-PB (pretreatment). 150 mg/kg DEN were administered to DEN,
positive control, treatment and pretreatment groups. After two weeks, 500
ppm PB applied to positive control and treatment groups. 50 mg/kg/day EA
were orally given to EA, and pretreatment groups for 8 weeks and treatment
group was given for four weeks. After 8 weeks rats were sacrificed, liver and
blood were subjected to biochemical evaluation.<p>
<b>Results: </b>DEN alone or with PB increased liver MDA levels significantly;
however plasma MDA levels didn?t change. EA pretreatment decreased liver
MDA levels (p<0.001). CAT activity did not change in the liver and erythrocytes
of the positive control group. Liver CAT activity increased insignificantly in
pretreatment and significantly in treatment group (p<0.05). SOD activity did
not change in liver and erythrocytes of DEN and positive control groups. EA
increased liver SOD activity significantly (p<0.001). DEN application alone or
with PB decreased liver and blood GSH levels significantly (p<0.05), while EA
application increased liver GSH levels in treatment (p<0.05). Blood GSH levels
decreased significantly in treatment (p<0.001).<p>
<b>Conclusion: </b>It is considered that EA pretreatment could be more effective
than EA treatment in DEN, PB induced oxidative stress in rats. |
| format | Article |
| id | doaj-art-f76e32c043a94b8da5bbb4403a26ea25 |
| institution | Kabale University |
| issn | 1309-6958 2146-1953 |
| language | English |
| publishDate | 2022-12-01 |
| publisher | Selcuk University Press |
| record_format | Article |
| series | Eurasian Journal of Veterinary Sciences |
| spelling | doaj-art-f76e32c043a94b8da5bbb4403a26ea252025-01-02T22:36:18ZengSelcuk University PressEurasian Journal of Veterinary Sciences1309-69582146-19532022-12-013842252311407Ellagic acid could prevent experimentally induced oxidative stress in ratsMehmet Ali Kisacam0Gonca Ozan KocamuftuogluEnver Ozan1Sema Temizer Ozan2Mustafa Kemal University, Veterinary Faculty, Department of Biochemistry, Hatay, TurkeyFirat University, Medicine Faculty, Department of Histology and Embryology, Konya, TurkeyFirat University, Veterinary Faculty, Department of Biochemistry, Konya, Turkey<b>Aim:</b> Reactive oxygen species (ROS) are produced during the metabolism of diethylnitrosamine (DEN) and phenobarbital (PB). Ellagic acid (EA) is a natural biologically active polyphenol with antioxidant activity. The present study was conducted to assess the preventive and protective capability of EA on DEN-PB-induced oxidative damage in rats.<p> <b>Materials and Methods:</b> 60 Sprague-Dawley rats were assigned into 6 groups: Negative control, DEN, EA, DEN-PB (positive control), DEN-PB-EA (treatment), and EA-DEN-PB (pretreatment). 150 mg/kg DEN were administered to DEN, positive control, treatment and pretreatment groups. After two weeks, 500 ppm PB applied to positive control and treatment groups. 50 mg/kg/day EA were orally given to EA, and pretreatment groups for 8 weeks and treatment group was given for four weeks. After 8 weeks rats were sacrificed, liver and blood were subjected to biochemical evaluation.<p> <b>Results: </b>DEN alone or with PB increased liver MDA levels significantly; however plasma MDA levels didn?t change. EA pretreatment decreased liver MDA levels (p<0.001). CAT activity did not change in the liver and erythrocytes of the positive control group. Liver CAT activity increased insignificantly in pretreatment and significantly in treatment group (p<0.05). SOD activity did not change in liver and erythrocytes of DEN and positive control groups. EA increased liver SOD activity significantly (p<0.001). DEN application alone or with PB decreased liver and blood GSH levels significantly (p<0.05), while EA application increased liver GSH levels in treatment (p<0.05). Blood GSH levels decreased significantly in treatment (p<0.001).<p> <b>Conclusion: </b>It is considered that EA pretreatment could be more effective than EA treatment in DEN, PB induced oxidative stress in rats.http://eurasianjvetsci.org/pdf.php3?id=1407diethylnitrosamineellagic acidoxidative stressphenobarbital |
| spellingShingle | Mehmet Ali Kisacam Gonca Ozan Kocamuftuoglu Enver Ozan Sema Temizer Ozan Ellagic acid could prevent experimentally induced oxidative stress in rats Eurasian Journal of Veterinary Sciences diethylnitrosamine ellagic acid oxidative stress phenobarbital |
| title | Ellagic acid could prevent experimentally induced oxidative stress in rats |
| title_full | Ellagic acid could prevent experimentally induced oxidative stress in rats |
| title_fullStr | Ellagic acid could prevent experimentally induced oxidative stress in rats |
| title_full_unstemmed | Ellagic acid could prevent experimentally induced oxidative stress in rats |
| title_short | Ellagic acid could prevent experimentally induced oxidative stress in rats |
| title_sort | ellagic acid could prevent experimentally induced oxidative stress in rats |
| topic | diethylnitrosamine ellagic acid oxidative stress phenobarbital |
| url | http://eurasianjvetsci.org/pdf.php3?id=1407 |
| work_keys_str_mv | AT mehmetalikisacam ellagicacidcouldpreventexperimentallyinducedoxidativestressinrats AT goncaozankocamuftuoglu ellagicacidcouldpreventexperimentallyinducedoxidativestressinrats AT enverozan ellagicacidcouldpreventexperimentallyinducedoxidativestressinrats AT sematemizerozan ellagicacidcouldpreventexperimentallyinducedoxidativestressinrats |