Ellagic acid could prevent experimentally induced oxidative stress in rats
<b>Aim:</b> Reactive oxygen species (ROS) are produced during the metabolism of diethylnitrosamine (DEN) and phenobarbital (PB). Ellagic acid (EA) is a natural biologically active polyphenol with antioxidant activity. The present study was conducted to assess the preventive and protectiv...
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| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Selcuk University Press
2022-12-01
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| Series: | Eurasian Journal of Veterinary Sciences |
| Subjects: | |
| Online Access: | http://eurasianjvetsci.org/pdf.php3?id=1407 |
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| Summary: | <b>Aim:</b> Reactive oxygen species (ROS) are produced during the metabolism
of diethylnitrosamine (DEN) and phenobarbital (PB). Ellagic acid (EA) is a
natural biologically active polyphenol with antioxidant activity. The present
study was conducted to assess the preventive and protective capability of EA
on DEN-PB-induced oxidative damage in rats.<p>
<b>Materials and Methods:</b> 60 Sprague-Dawley rats were assigned into 6 groups:
Negative control, DEN, EA, DEN-PB (positive control), DEN-PB-EA (treatment),
and EA-DEN-PB (pretreatment). 150 mg/kg DEN were administered to DEN,
positive control, treatment and pretreatment groups. After two weeks, 500
ppm PB applied to positive control and treatment groups. 50 mg/kg/day EA
were orally given to EA, and pretreatment groups for 8 weeks and treatment
group was given for four weeks. After 8 weeks rats were sacrificed, liver and
blood were subjected to biochemical evaluation.<p>
<b>Results: </b>DEN alone or with PB increased liver MDA levels significantly;
however plasma MDA levels didn?t change. EA pretreatment decreased liver
MDA levels (p<0.001). CAT activity did not change in the liver and erythrocytes
of the positive control group. Liver CAT activity increased insignificantly in
pretreatment and significantly in treatment group (p<0.05). SOD activity did
not change in liver and erythrocytes of DEN and positive control groups. EA
increased liver SOD activity significantly (p<0.001). DEN application alone or
with PB decreased liver and blood GSH levels significantly (p<0.05), while EA
application increased liver GSH levels in treatment (p<0.05). Blood GSH levels
decreased significantly in treatment (p<0.001).<p>
<b>Conclusion: </b>It is considered that EA pretreatment could be more effective
than EA treatment in DEN, PB induced oxidative stress in rats. |
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| ISSN: | 1309-6958 2146-1953 |