Effects of drugs on kinetic values of cytokines, adenosine deaminase and 13,14-dihydro-15-keto-prostaglandin f<sub>2α</sub> in endotoxemia: a different approach
Aim: The objective of this study was to determine the effects of enrofloxacin (ENR), flunixin meglumine (FM) and dexamethasone (DEX) on kinetic values of cytokines, adenosine deaminase (ADA) and 13,14-dihydro-15-keto-prostaglandin F<sub>2a</sub> (PGM) in lipopolysaccharide-induced endoto...
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| Format: | Article |
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Selcuk University Press
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| Series: | Eurasian Journal of Veterinary Sciences |
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| Online Access: | http://eurasianjvetsci.org/pdf.php3?id=553 |
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| author | Feray Altan Muammer Elmas Ayse Er Kamil Uney Gul Cetin Bunyamin Tras Enver Yazar |
| author_facet | Feray Altan Muammer Elmas Ayse Er Kamil Uney Gul Cetin Bunyamin Tras Enver Yazar |
| author_sort | Feray Altan |
| collection | DOAJ |
| description | Aim: The objective of this study was to determine the effects
of enrofloxacin (ENR), flunixin meglumine (FM) and dexamethasone
(DEX) on kinetic values of cytokines, adenosine
deaminase (ADA) and 13,14-dihydro-15-keto-prostaglandin
F<sub>2a</sub> (PGM) in lipopolysaccharide-induced endotoxemia.
Materials and Methods: Rats were divided into seven
groups. To induce endotoxemia, lipopolysaccharide (LPS)
was injected into all groups, including the positive control.
The six other groups received the following drugs: ENR,
FM, low-dose DEX, high-dose DEX, ENR + FM + low-dose
DEX and ENR + FM + high-dose DEX. After the treatments,
blood samples were collected at 0, 1, 2, 4, 6, 8, 12, 24, and
48 hours. Serum tumor necrosis factor alpha (TNFα), interleukin-
6 (IL-6), interleukin-10 (IL-10), ADA and plasma
PGM levels were determined by ELISA. Area under the concentration
time curve (AUC<sub>0-48</sub>), maximal concentration in
plasma or serum (C<sub>max</sub>) and time to reach (t<sub>max</sub>) values were
determined by pharmacokinetic computer program.
Results: ENR increased (p<0.05) AUC<sub>0-48</sub> of PGM and decreased
(p<0.05) AUC<sub>0-48</sub> of IL-6, IL-10 and ADA, while FM
decreased (p<0.05) AUC<sub>0-48</sub> of IL-6 and ADA compared
to LPS group. DEX alone and combined administrations
caused the lower AUC<sub>0-48</sub> of all values (p<0.05).
Conclusion: Kinetic values, especially AUC, may be used
for total evaluation of endotoxemia markers determined at
different sampling times in same groups as a different and
logical approach. |
| format | Article |
| id | doaj-art-f76c98f2737940779f88bc932e900bdc |
| institution | Kabale University |
| issn | 1309-6958 2146-1953 |
| language | English |
| publisher | Selcuk University Press |
| record_format | Article |
| series | Eurasian Journal of Veterinary Sciences |
| spelling | doaj-art-f76c98f2737940779f88bc932e900bdc2025-01-02T22:36:20ZengSelcuk University PressEurasian Journal of Veterinary Sciences1309-69582146-19532611519553Effects of drugs on kinetic values of cytokines, adenosine deaminase and 13,14-dihydro-15-keto-prostaglandin f<sub>2α</sub> in endotoxemia: a different approachFeray Altan0Muammer Elmas1Ayse Er2Kamil Uney3Gul Cetin4Bunyamin Tras5Enver Yazar6Department of Pharmacology and Toxicology, Faculty of Veterinary Medicine, University of Dicle, Campus, Diyarbakir, TurkeyDepartment of Pharmacology and Toxicology, Faculty of Veterinary Medicine, University of Selcuk, Campus, Konya, TurkeyDepartment of Pharmacology and Toxicology, Faculty of Veterinary Medicine, University of Selcuk, Campus, Konya, TurkeyDepartment of Pharmacology and Toxicology, Faculty of Veterinary Medicine, University of Selcuk, Campus, Konya, TurkeyDepartment of Pharmacology and Toxicology, Faculty of Veterinary Medicine, University of Mehmet Akif Ersoy, Burdur, TurkeyDepartment of Pharmacology and Toxicology, Faculty of Veterinary Medicine, University of Selcuk, Campus, Konya, TurkeyDepartment of Pharmacology and Toxicology, Faculty of Veterinary Medicine, University of Selcuk, Campus, Konya, TurkeyAim: The objective of this study was to determine the effects of enrofloxacin (ENR), flunixin meglumine (FM) and dexamethasone (DEX) on kinetic values of cytokines, adenosine deaminase (ADA) and 13,14-dihydro-15-keto-prostaglandin F<sub>2a</sub> (PGM) in lipopolysaccharide-induced endotoxemia. Materials and Methods: Rats were divided into seven groups. To induce endotoxemia, lipopolysaccharide (LPS) was injected into all groups, including the positive control. The six other groups received the following drugs: ENR, FM, low-dose DEX, high-dose DEX, ENR + FM + low-dose DEX and ENR + FM + high-dose DEX. After the treatments, blood samples were collected at 0, 1, 2, 4, 6, 8, 12, 24, and 48 hours. Serum tumor necrosis factor alpha (TNFα), interleukin- 6 (IL-6), interleukin-10 (IL-10), ADA and plasma PGM levels were determined by ELISA. Area under the concentration time curve (AUC<sub>0-48</sub>), maximal concentration in plasma or serum (C<sub>max</sub>) and time to reach (t<sub>max</sub>) values were determined by pharmacokinetic computer program. Results: ENR increased (p<0.05) AUC<sub>0-48</sub> of PGM and decreased (p<0.05) AUC<sub>0-48</sub> of IL-6, IL-10 and ADA, while FM decreased (p<0.05) AUC<sub>0-48</sub> of IL-6 and ADA compared to LPS group. DEX alone and combined administrations caused the lower AUC<sub>0-48</sub> of all values (p<0.05). Conclusion: Kinetic values, especially AUC, may be used for total evaluation of endotoxemia markers determined at different sampling times in same groups as a different and logical approach.http://eurasianjvetsci.org/pdf.php3?id=553enrofloxacinflunixindexamethasonekineticendotoxemia markers |
| spellingShingle | Feray Altan Muammer Elmas Ayse Er Kamil Uney Gul Cetin Bunyamin Tras Enver Yazar Effects of drugs on kinetic values of cytokines, adenosine deaminase and 13,14-dihydro-15-keto-prostaglandin f<sub>2α</sub> in endotoxemia: a different approach Eurasian Journal of Veterinary Sciences enrofloxacin flunixin dexamethasone kinetic endotoxemia markers |
| title | Effects of drugs on kinetic values of cytokines, adenosine deaminase and 13,14-dihydro-15-keto-prostaglandin f<sub>2α</sub> in endotoxemia: a different approach |
| title_full | Effects of drugs on kinetic values of cytokines, adenosine deaminase and 13,14-dihydro-15-keto-prostaglandin f<sub>2α</sub> in endotoxemia: a different approach |
| title_fullStr | Effects of drugs on kinetic values of cytokines, adenosine deaminase and 13,14-dihydro-15-keto-prostaglandin f<sub>2α</sub> in endotoxemia: a different approach |
| title_full_unstemmed | Effects of drugs on kinetic values of cytokines, adenosine deaminase and 13,14-dihydro-15-keto-prostaglandin f<sub>2α</sub> in endotoxemia: a different approach |
| title_short | Effects of drugs on kinetic values of cytokines, adenosine deaminase and 13,14-dihydro-15-keto-prostaglandin f<sub>2α</sub> in endotoxemia: a different approach |
| title_sort | effects of drugs on kinetic values of cytokines adenosine deaminase and 13 14 dihydro 15 keto prostaglandin f lt sub gt 2 945 lt sub gt in endotoxemia a different approach |
| topic | enrofloxacin flunixin dexamethasone kinetic endotoxemia markers |
| url | http://eurasianjvetsci.org/pdf.php3?id=553 |
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