Prevalence of multi-drug resistant bacteria in intensive care units at Tripoli University Hospital, Tripoli, Libya

Among hospitalized patients worldwide, infections caused by multidrug-resistant (MDR) bacteria are a major cause of morbidity and mortality. This study aimed to isolate MDR bacteria from five intensive care units (ICUs) at Tripoli University Hospital (TUH). A prospective cross-sectional study was co...

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Main Authors: Khaled Ibrahim, Dalal Thwood, Hajer ELgheriani, Mohamed Salem, Zaynab Elgadiym, Ahmed Zaghdani, Inas Alhudiri, Abdulraouf Habibi, Abdurrezagh Elfahem, Saadeddin Belaid, Otman Ermithi, Mahmoud Almaghrabi, Abubaker ELmaryul, Suad Almadah, Abdunnabi Rayes, Salah Edin El Meshri, Allaaeddin El Salabi, Adam Elzagheid
Format: Article
Language:English
Published: Taylor & Francis Group 2024-12-01
Series:Libyan Journal of Medicine
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Online Access:https://www.tandfonline.com/doi/10.1080/19932820.2024.2348235
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author Khaled Ibrahim
Dalal Thwood
Hajer ELgheriani
Mohamed Salem
Zaynab Elgadiym
Ahmed Zaghdani
Inas Alhudiri
Abdulraouf Habibi
Abdurrezagh Elfahem
Saadeddin Belaid
Otman Ermithi
Mahmoud Almaghrabi
Abubaker ELmaryul
Suad Almadah
Abdunnabi Rayes
Salah Edin El Meshri
Allaaeddin El Salabi
Adam Elzagheid
author_facet Khaled Ibrahim
Dalal Thwood
Hajer ELgheriani
Mohamed Salem
Zaynab Elgadiym
Ahmed Zaghdani
Inas Alhudiri
Abdulraouf Habibi
Abdurrezagh Elfahem
Saadeddin Belaid
Otman Ermithi
Mahmoud Almaghrabi
Abubaker ELmaryul
Suad Almadah
Abdunnabi Rayes
Salah Edin El Meshri
Allaaeddin El Salabi
Adam Elzagheid
author_sort Khaled Ibrahim
collection DOAJ
description Among hospitalized patients worldwide, infections caused by multidrug-resistant (MDR) bacteria are a major cause of morbidity and mortality. This study aimed to isolate MDR bacteria from five intensive care units (ICUs) at Tripoli University Hospital (TUH). A prospective cross-sectional study was conducted over a seven-month period (September 2022 to March 2023) across five ICUs at TUH. A total of 197 swabs were collected from Patients’, healthcare workers’ and ICUs equipment. Samples collected from patients were nasal swabs, oral cavity swabs, hand swabs, sputum specimens, skin swabs, umbilical venous catheter swabs, and around cannula. Swabs collected from health care workers were nasal swabs, whereas ICUs equipment’s samples were from endotracheal tubes, oxygen masks, and neonatal incubators. Identification and antimicrobial susceptibility test was confirmed by using MicroScan auto SCAN 4 (Beckman Coulter). The most frequent strains were Gram negative bacilli 113 (57.4%) with the predominance of Acinetobacter baumannii 50/113 (44%) followed by Klebsiella pneumoniae 44/113 (40%) and Pseudomonas aeruginosa 6/113 (5.3%). The total Gram positive bacterial strains isolated were 84 (42.6%), coagulase negative Staphylococci 55 (66%) with MDRs (89%) were the most common isolates followed by Staphylococcus aureus 15 (17.8%). Different antibiotics were used against these isolates; Gram- negative isolates showed high resistance rates to ceftazidime, gentamicin, amikacin and ertapenem. A. baumannii were the most frequent MDROs (94%), and the highest resistance rates in Gram-positive strains were observed toward ampicillin, oxacillin, ampicillin/sulbactam and Cefoxitin, representing 90% of total MDR Gram-positive isolates. ESBL and MRS were identified in most of strains. The prevalence of antibiotic resistance was high for both Gram negative and Gram positive isolates. This prevalence requires strict infection prevention and control intervention, continuous monitoring, implementation of effective antibiotic stewardship, immediate, concerted and collaborative action to monitor its prevalence and spread in the hospital.
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spelling doaj-art-f7378db281ec4ad1a45d26c322211a792024-11-29T11:24:42ZengTaylor & Francis GroupLibyan Journal of Medicine1993-28201819-63572024-12-0119110.1080/19932820.2024.2348235Prevalence of multi-drug resistant bacteria in intensive care units at Tripoli University Hospital, Tripoli, LibyaKhaled Ibrahim0Dalal Thwood1Hajer ELgheriani2Mohamed Salem3Zaynab Elgadiym4Ahmed Zaghdani5Inas Alhudiri6Abdulraouf Habibi7Abdurrezagh Elfahem8Saadeddin Belaid9Otman Ermithi10Mahmoud Almaghrabi11Abubaker ELmaryul12Suad Almadah13Abdunnabi Rayes14Salah Edin El Meshri15Allaaeddin El Salabi16Adam Elzagheid17Department of Microbiology, Libyan Biotechnology Research Center, Tripoli, LibyaDepartment of Microbiology, Libyan Biotechnology Research Center, Tripoli, LibyaDepartment of Microbiology, Libyan Biotechnology Research Center, Tripoli, LibyaDepartment of Microbiology, Libyan Biotechnology Research Center, Tripoli, LibyaDepartment of Microbiology, Libyan Biotechnology Research Center, Tripoli, LibyaDepartment of Microbiology, Libyan Biotechnology Research Center, Tripoli, LibyaDepartment of Microbiology, Libyan Biotechnology Research Center, Tripoli, LibyaDepartment of Microbiology, Libyan Biotechnology Research Center, Tripoli, LibyaDepartment of Microbiology, Libyan Biotechnology Research Center, Tripoli, LibyaDepartment of Microbiology, Libyan Biotechnology Research Center, Tripoli, LibyaDepartment of Microbiology, Libyan Biotechnology Research Center, Tripoli, LibyaPediatric Intensive Care Unit, Tripoli University Hospital, Tripoli, LibyaMedical Intensive Care Unit, Tripoli University Hospital, Tripoli, LibyaNeonatal Intensive Care Unit, Tripoli University Hospital, Tripoli, LibyaInfection Control Office, Tripoli University Hospital, Tripoli, LibyaDepartment of Microbiology, Libyan Biotechnology Research Center, Tripoli, LibyaDepartment of Environmental Health, Faculty of Public Health, University of Benghazi, Benghazi, LibyaDepartment of Microbiology, Libyan Biotechnology Research Center, Tripoli, LibyaAmong hospitalized patients worldwide, infections caused by multidrug-resistant (MDR) bacteria are a major cause of morbidity and mortality. This study aimed to isolate MDR bacteria from five intensive care units (ICUs) at Tripoli University Hospital (TUH). A prospective cross-sectional study was conducted over a seven-month period (September 2022 to March 2023) across five ICUs at TUH. A total of 197 swabs were collected from Patients’, healthcare workers’ and ICUs equipment. Samples collected from patients were nasal swabs, oral cavity swabs, hand swabs, sputum specimens, skin swabs, umbilical venous catheter swabs, and around cannula. Swabs collected from health care workers were nasal swabs, whereas ICUs equipment’s samples were from endotracheal tubes, oxygen masks, and neonatal incubators. Identification and antimicrobial susceptibility test was confirmed by using MicroScan auto SCAN 4 (Beckman Coulter). The most frequent strains were Gram negative bacilli 113 (57.4%) with the predominance of Acinetobacter baumannii 50/113 (44%) followed by Klebsiella pneumoniae 44/113 (40%) and Pseudomonas aeruginosa 6/113 (5.3%). The total Gram positive bacterial strains isolated were 84 (42.6%), coagulase negative Staphylococci 55 (66%) with MDRs (89%) were the most common isolates followed by Staphylococcus aureus 15 (17.8%). Different antibiotics were used against these isolates; Gram- negative isolates showed high resistance rates to ceftazidime, gentamicin, amikacin and ertapenem. A. baumannii were the most frequent MDROs (94%), and the highest resistance rates in Gram-positive strains were observed toward ampicillin, oxacillin, ampicillin/sulbactam and Cefoxitin, representing 90% of total MDR Gram-positive isolates. ESBL and MRS were identified in most of strains. The prevalence of antibiotic resistance was high for both Gram negative and Gram positive isolates. This prevalence requires strict infection prevention and control intervention, continuous monitoring, implementation of effective antibiotic stewardship, immediate, concerted and collaborative action to monitor its prevalence and spread in the hospital.https://www.tandfonline.com/doi/10.1080/19932820.2024.2348235Multi-drug resistanceICUantimicrobial susceptibilityTripoli University HospitalLibya
spellingShingle Khaled Ibrahim
Dalal Thwood
Hajer ELgheriani
Mohamed Salem
Zaynab Elgadiym
Ahmed Zaghdani
Inas Alhudiri
Abdulraouf Habibi
Abdurrezagh Elfahem
Saadeddin Belaid
Otman Ermithi
Mahmoud Almaghrabi
Abubaker ELmaryul
Suad Almadah
Abdunnabi Rayes
Salah Edin El Meshri
Allaaeddin El Salabi
Adam Elzagheid
Prevalence of multi-drug resistant bacteria in intensive care units at Tripoli University Hospital, Tripoli, Libya
Libyan Journal of Medicine
Multi-drug resistance
ICU
antimicrobial susceptibility
Tripoli University Hospital
Libya
title Prevalence of multi-drug resistant bacteria in intensive care units at Tripoli University Hospital, Tripoli, Libya
title_full Prevalence of multi-drug resistant bacteria in intensive care units at Tripoli University Hospital, Tripoli, Libya
title_fullStr Prevalence of multi-drug resistant bacteria in intensive care units at Tripoli University Hospital, Tripoli, Libya
title_full_unstemmed Prevalence of multi-drug resistant bacteria in intensive care units at Tripoli University Hospital, Tripoli, Libya
title_short Prevalence of multi-drug resistant bacteria in intensive care units at Tripoli University Hospital, Tripoli, Libya
title_sort prevalence of multi drug resistant bacteria in intensive care units at tripoli university hospital tripoli libya
topic Multi-drug resistance
ICU
antimicrobial susceptibility
Tripoli University Hospital
Libya
url https://www.tandfonline.com/doi/10.1080/19932820.2024.2348235
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