Inhibition of macrophage polarization and pyroptosis in collagen-induced arthritis through MSC-exo and ginsenoside Rh2
Abstract Background Rheumatoid arthritis (RA) is a chronic autoimmune disease characterized by joint inflammation, tissue damage, and fibrosis, significantly affecting the quality of life. While there are currently some effective treatments available, they often come with side effects. There is an u...
Saved in:
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
BMC
2025-01-01
|
| Series: | Arthritis Research & Therapy |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s13075-025-03473-3 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1841544481277476864 |
|---|---|
| author | Zhongsheng Zhou Shuhui Wu Yang Li Pu Shao Jinlan Jiang |
| author_facet | Zhongsheng Zhou Shuhui Wu Yang Li Pu Shao Jinlan Jiang |
| author_sort | Zhongsheng Zhou |
| collection | DOAJ |
| description | Abstract Background Rheumatoid arthritis (RA) is a chronic autoimmune disease characterized by joint inflammation, tissue damage, and fibrosis, significantly affecting the quality of life. While there are currently some effective treatments available, they often come with side effects. There is an urgent need to find new treatments that can further improve therapeutic outcomes and reduce side effects. Methods Our study investigates the role of Mesenchymal Stem Cell exosomes (MSC-exo) combined with Ginsenoside Rh2 (Rh2) in the treatment of RA. We specifically focus on how this combined strategy influences macrophage polarization and pyroptosis. This research utilized a collagen-induced rat arthritis model. Results The study findings reveal that the combination of MSC-exo combined with Rh2 can inhibit the polarization of M1 macrophages, increase the proportion of M2-like macrophages, and suppress M1-like macrophage pyroptosis via the NLRP3/Caspase11/GSDMD-N pathway. In the rat arthritis model, the combination of MSC-exo and Rh2 showed synergistic therapeutic effects. Conclusion This research contributes to a deeper understanding of RA’s pathogenesis and presents new potential targeted therapeutic interventions. The combined application of MSC-exo and Rh2 offers promising insights for future innovative strategies in RA treatment, paving the way for more effective management of this autoimmune disease. |
| format | Article |
| id | doaj-art-f7282e9e98df414ca230baa1f9310066 |
| institution | Kabale University |
| issn | 1478-6362 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | BMC |
| record_format | Article |
| series | Arthritis Research & Therapy |
| spelling | doaj-art-f7282e9e98df414ca230baa1f93100662025-01-12T12:34:03ZengBMCArthritis Research & Therapy1478-63622025-01-0127112210.1186/s13075-025-03473-3Inhibition of macrophage polarization and pyroptosis in collagen-induced arthritis through MSC-exo and ginsenoside Rh2Zhongsheng Zhou0Shuhui Wu1Yang Li2Pu Shao3Jinlan Jiang4Scientific Research Center, China-Japan Union Hospital of Jilin UniversityScientific Research Center, China-Japan Union Hospital of Jilin UniversityScientific Research Center, China-Japan Union Hospital of Jilin UniversityDepartment of Orthopedics, China-Japan Union Hospital of Jilin UniversityScientific Research Center, China-Japan Union Hospital of Jilin UniversityAbstract Background Rheumatoid arthritis (RA) is a chronic autoimmune disease characterized by joint inflammation, tissue damage, and fibrosis, significantly affecting the quality of life. While there are currently some effective treatments available, they often come with side effects. There is an urgent need to find new treatments that can further improve therapeutic outcomes and reduce side effects. Methods Our study investigates the role of Mesenchymal Stem Cell exosomes (MSC-exo) combined with Ginsenoside Rh2 (Rh2) in the treatment of RA. We specifically focus on how this combined strategy influences macrophage polarization and pyroptosis. This research utilized a collagen-induced rat arthritis model. Results The study findings reveal that the combination of MSC-exo combined with Rh2 can inhibit the polarization of M1 macrophages, increase the proportion of M2-like macrophages, and suppress M1-like macrophage pyroptosis via the NLRP3/Caspase11/GSDMD-N pathway. In the rat arthritis model, the combination of MSC-exo and Rh2 showed synergistic therapeutic effects. Conclusion This research contributes to a deeper understanding of RA’s pathogenesis and presents new potential targeted therapeutic interventions. The combined application of MSC-exo and Rh2 offers promising insights for future innovative strategies in RA treatment, paving the way for more effective management of this autoimmune disease.https://doi.org/10.1186/s13075-025-03473-3Collagen-induced arthritisMesenchymal stem cell exosomesM1-like macrophagesM2-like macrophagesNLRP3/Caspase11/GSDMD-N pathwayGinsenoside Rh2 |
| spellingShingle | Zhongsheng Zhou Shuhui Wu Yang Li Pu Shao Jinlan Jiang Inhibition of macrophage polarization and pyroptosis in collagen-induced arthritis through MSC-exo and ginsenoside Rh2 Arthritis Research & Therapy Collagen-induced arthritis Mesenchymal stem cell exosomes M1-like macrophages M2-like macrophages NLRP3/Caspase11/GSDMD-N pathway Ginsenoside Rh2 |
| title | Inhibition of macrophage polarization and pyroptosis in collagen-induced arthritis through MSC-exo and ginsenoside Rh2 |
| title_full | Inhibition of macrophage polarization and pyroptosis in collagen-induced arthritis through MSC-exo and ginsenoside Rh2 |
| title_fullStr | Inhibition of macrophage polarization and pyroptosis in collagen-induced arthritis through MSC-exo and ginsenoside Rh2 |
| title_full_unstemmed | Inhibition of macrophage polarization and pyroptosis in collagen-induced arthritis through MSC-exo and ginsenoside Rh2 |
| title_short | Inhibition of macrophage polarization and pyroptosis in collagen-induced arthritis through MSC-exo and ginsenoside Rh2 |
| title_sort | inhibition of macrophage polarization and pyroptosis in collagen induced arthritis through msc exo and ginsenoside rh2 |
| topic | Collagen-induced arthritis Mesenchymal stem cell exosomes M1-like macrophages M2-like macrophages NLRP3/Caspase11/GSDMD-N pathway Ginsenoside Rh2 |
| url | https://doi.org/10.1186/s13075-025-03473-3 |
| work_keys_str_mv | AT zhongshengzhou inhibitionofmacrophagepolarizationandpyroptosisincollageninducedarthritisthroughmscexoandginsenosiderh2 AT shuhuiwu inhibitionofmacrophagepolarizationandpyroptosisincollageninducedarthritisthroughmscexoandginsenosiderh2 AT yangli inhibitionofmacrophagepolarizationandpyroptosisincollageninducedarthritisthroughmscexoandginsenosiderh2 AT pushao inhibitionofmacrophagepolarizationandpyroptosisincollageninducedarthritisthroughmscexoandginsenosiderh2 AT jinlanjiang inhibitionofmacrophagepolarizationandpyroptosisincollageninducedarthritisthroughmscexoandginsenosiderh2 |