Transcriptomic and Metabolomic Analysis Reveal the Effects of Light Quality on the Growth and Lipid Biosynthesis in <i>Chlorella pyrenoidosa</i>
Light quality has significant effects on the growth and metabolite accumulation of algal cells. However, the related mechanism has not been fully elucidated. This study reveals that both red and blue light can promote the growth and biomass accumulation of <i>Chlorella pyrenoidosa</i>, w...
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MDPI AG
2024-09-01
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| Series: | Biomolecules |
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| Online Access: | https://www.mdpi.com/2218-273X/14/9/1144 |
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| author | Tingting Zhu Ge Guan Lele Huang Lina Wen Linxuan Li Maozhi Ren |
| author_facet | Tingting Zhu Ge Guan Lele Huang Lina Wen Linxuan Li Maozhi Ren |
| author_sort | Tingting Zhu |
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| description | Light quality has significant effects on the growth and metabolite accumulation of algal cells. However, the related mechanism has not been fully elucidated. This study reveals that both red and blue light can promote the growth and biomass accumulation of <i>Chlorella pyrenoidosa</i>, with the enhancing effect of blue light being more pronounced. Cultivation under blue light reduced the content of total carbohydrate in <i>Chlorella pyrenoidosa</i>, while increasing the content of protein and lipid. Conversely, red light decreased the content of protein and increased the content of carbohydrate and lipid. Blue light induces a shift in carbon flux from carbohydrate to protein, while red light transfers carbon flux from protein to lipid. Transcriptomic and metabolomic analysis indicated that both red and blue light positively regulate lipid synthesis in <i>Chlorella pyrenoidosa</i>, but they exhibited distinct impacts on the fatty acid compositions. These findings suggest that manipulating light qualities can modulate carbon metabolic pathways, potentially converting protein into lipid in <i>Chlorella pyrenoidosa</i>. |
| format | Article |
| id | doaj-art-f70e423e426a44c788bf8cc3f8dd5df0 |
| institution | OA Journals |
| issn | 2218-273X |
| language | English |
| publishDate | 2024-09-01 |
| publisher | MDPI AG |
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| series | Biomolecules |
| spelling | doaj-art-f70e423e426a44c788bf8cc3f8dd5df02025-08-20T01:56:05ZengMDPI AGBiomolecules2218-273X2024-09-01149114410.3390/biom14091144Transcriptomic and Metabolomic Analysis Reveal the Effects of Light Quality on the Growth and Lipid Biosynthesis in <i>Chlorella pyrenoidosa</i>Tingting Zhu0Ge Guan1Lele Huang2Lina Wen3Linxuan Li4Maozhi Ren5Institute of Urban Agriculture, Chinese Academy of Agricultural Sciences, Chengdu National Agricultural Science and Technology Center, Chengdu 610213, ChinaSchool of Agricultural Science, Zhengzhou University, Zhengzhou 450001, ChinaInstitute of Urban Agriculture, Chinese Academy of Agricultural Sciences, Chengdu National Agricultural Science and Technology Center, Chengdu 610213, ChinaSchool of Agricultural Science, Zhengzhou University, Zhengzhou 450001, ChinaInstitute of Urban Agriculture, Chinese Academy of Agricultural Sciences, Chengdu National Agricultural Science and Technology Center, Chengdu 610213, ChinaInstitute of Urban Agriculture, Chinese Academy of Agricultural Sciences, Chengdu National Agricultural Science and Technology Center, Chengdu 610213, ChinaLight quality has significant effects on the growth and metabolite accumulation of algal cells. However, the related mechanism has not been fully elucidated. This study reveals that both red and blue light can promote the growth and biomass accumulation of <i>Chlorella pyrenoidosa</i>, with the enhancing effect of blue light being more pronounced. Cultivation under blue light reduced the content of total carbohydrate in <i>Chlorella pyrenoidosa</i>, while increasing the content of protein and lipid. Conversely, red light decreased the content of protein and increased the content of carbohydrate and lipid. Blue light induces a shift in carbon flux from carbohydrate to protein, while red light transfers carbon flux from protein to lipid. Transcriptomic and metabolomic analysis indicated that both red and blue light positively regulate lipid synthesis in <i>Chlorella pyrenoidosa</i>, but they exhibited distinct impacts on the fatty acid compositions. These findings suggest that manipulating light qualities can modulate carbon metabolic pathways, potentially converting protein into lipid in <i>Chlorella pyrenoidosa</i>.https://www.mdpi.com/2218-273X/14/9/1144light qualitybiomasslipid biosynthesisomics analysis<i>Chlorella pyrenoidosa</i> |
| spellingShingle | Tingting Zhu Ge Guan Lele Huang Lina Wen Linxuan Li Maozhi Ren Transcriptomic and Metabolomic Analysis Reveal the Effects of Light Quality on the Growth and Lipid Biosynthesis in <i>Chlorella pyrenoidosa</i> Biomolecules light quality biomass lipid biosynthesis omics analysis <i>Chlorella pyrenoidosa</i> |
| title | Transcriptomic and Metabolomic Analysis Reveal the Effects of Light Quality on the Growth and Lipid Biosynthesis in <i>Chlorella pyrenoidosa</i> |
| title_full | Transcriptomic and Metabolomic Analysis Reveal the Effects of Light Quality on the Growth and Lipid Biosynthesis in <i>Chlorella pyrenoidosa</i> |
| title_fullStr | Transcriptomic and Metabolomic Analysis Reveal the Effects of Light Quality on the Growth and Lipid Biosynthesis in <i>Chlorella pyrenoidosa</i> |
| title_full_unstemmed | Transcriptomic and Metabolomic Analysis Reveal the Effects of Light Quality on the Growth and Lipid Biosynthesis in <i>Chlorella pyrenoidosa</i> |
| title_short | Transcriptomic and Metabolomic Analysis Reveal the Effects of Light Quality on the Growth and Lipid Biosynthesis in <i>Chlorella pyrenoidosa</i> |
| title_sort | transcriptomic and metabolomic analysis reveal the effects of light quality on the growth and lipid biosynthesis in i chlorella pyrenoidosa i |
| topic | light quality biomass lipid biosynthesis omics analysis <i>Chlorella pyrenoidosa</i> |
| url | https://www.mdpi.com/2218-273X/14/9/1144 |
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