Investigation into Critical Gut Microbes Influencing Intramuscular Fat Deposition in Min Pigs
To determine the pivotal microorganisms affecting intramuscular fat (IMF) accumulation in Min pigs and to discern the extent of the influence exerted by various intestinal segments on IMF-related traits, we sequenced 16S rRNA from the contents of six intestinal segments from a high IMF group (Group...
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2024-10-01
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| author | Long Jin Ke Li Zhimin Li Xuankai Huang Li Wang Xibiao Wang Shengwei Di Shiquan Cui Yuan Xu |
| author_facet | Long Jin Ke Li Zhimin Li Xuankai Huang Li Wang Xibiao Wang Shengwei Di Shiquan Cui Yuan Xu |
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| description | To determine the pivotal microorganisms affecting intramuscular fat (IMF) accumulation in Min pigs and to discern the extent of the influence exerted by various intestinal segments on IMF-related traits, we sequenced 16S rRNA from the contents of six intestinal segments from a high IMF group (Group H) and a low IMF group (Group L) of Min pigs weighing 90 ± 1 kg. We then compared their diversity and disparities in bacterial genera. Group H exhibited considerably higher α diversity in the jejunum and colon than Group L (<i>p</i> < 0.05). When 95% confidence levels were considered, the main β diversity components for the ileum, caecum, and colon within Groups H and L exhibited absolute segregation. Accordingly, 31 differentially abundant genera across Group H were pinpointed via LEfSe and the Wilcoxon test (<i>p</i> < 0.05) and subsequently scrutinised based on their distribution and abundance across distinct intestinal segments and their correlation with IMF phenotypes. The abundances of <i>Terrisporobacter</i>, <i>Acetitomaculum</i>, <i>Bacteroides</i>, <i>Fibrobacter</i>, <i>Treponema</i>, <i>Akkermansia</i>, <i>Blautia</i>, <i>Clostridium sensu stricto 1</i>, <i>Turicibacter</i>, <i>Subdoligranulum</i>, the <i>[Eubacterium] siraeum group</i>, and <i>dgA 11 gut groups</i> were positively correlated with IMF content (<i>p</i> < 0.05), whereas those of <i>Bacillus</i>, the <i>Lachnospiraceae NK4A136 group</i>, <i>Streptococcus</i>, <i>Roseburia</i>, <i>Solobacterium</i>, <i>Veillonella</i>, <i>Lactobacillus</i>, the <i>Rikenellaceae RC9 gut group</i>, <i>Anaerovibrio</i>, and the <i>Lachnospiraceae AC2044 group</i> were negatively associated with IMF content (<i>p</i> < 0.05). Employing PICRUSt2 for predicting intergenic metabolic pathways that differ among intestinal microbial communities revealed that within the 95% confidence interval the colonic microbiome was enriched with the most metabolic pathways, including those related to lipid metabolism. The diversity results, bacterial genus distributions, and metabolic pathway disparities revealed the colonic segment as an influential region for IMF deposition. |
| format | Article |
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| spelling | doaj-art-f70d1b70d5d44dd38c256697f626fc8f2024-11-08T14:32:39ZengMDPI AGAnimals2076-26152024-10-011421312310.3390/ani14213123Investigation into Critical Gut Microbes Influencing Intramuscular Fat Deposition in Min PigsLong Jin0Ke Li1Zhimin Li2Xuankai Huang3Li Wang4Xibiao Wang5Shengwei Di6Shiquan Cui7Yuan Xu8College of Animal Science and Technology, Northeast Agricultural University, No. 600 Changjiang Road, Xiangfang District, Harbin 150030, ChinaCollege of Animal Science and Technology, Northeast Agricultural University, No. 600 Changjiang Road, Xiangfang District, Harbin 150030, ChinaCollege of Animal Science and Technology, Northeast Agricultural University, No. 600 Changjiang Road, Xiangfang District, Harbin 150030, ChinaCollege of Animal Science and Technology, Northeast Agricultural University, No. 600 Changjiang Road, Xiangfang District, Harbin 150030, ChinaCollege of Animal Science and Technology, Northeast Agricultural University, No. 600 Changjiang Road, Xiangfang District, Harbin 150030, ChinaCollege of Animal Science and Technology, Northeast Agricultural University, No. 600 Changjiang Road, Xiangfang District, Harbin 150030, ChinaCollege of Animal Science and Technology, Northeast Agricultural University, No. 600 Changjiang Road, Xiangfang District, Harbin 150030, ChinaCollege of Animal Science and Technology, Northeast Agricultural University, No. 600 Changjiang Road, Xiangfang District, Harbin 150030, ChinaCollege of Animal Science and Technology, Northeast Agricultural University, No. 600 Changjiang Road, Xiangfang District, Harbin 150030, ChinaTo determine the pivotal microorganisms affecting intramuscular fat (IMF) accumulation in Min pigs and to discern the extent of the influence exerted by various intestinal segments on IMF-related traits, we sequenced 16S rRNA from the contents of six intestinal segments from a high IMF group (Group H) and a low IMF group (Group L) of Min pigs weighing 90 ± 1 kg. We then compared their diversity and disparities in bacterial genera. Group H exhibited considerably higher α diversity in the jejunum and colon than Group L (<i>p</i> < 0.05). When 95% confidence levels were considered, the main β diversity components for the ileum, caecum, and colon within Groups H and L exhibited absolute segregation. Accordingly, 31 differentially abundant genera across Group H were pinpointed via LEfSe and the Wilcoxon test (<i>p</i> < 0.05) and subsequently scrutinised based on their distribution and abundance across distinct intestinal segments and their correlation with IMF phenotypes. The abundances of <i>Terrisporobacter</i>, <i>Acetitomaculum</i>, <i>Bacteroides</i>, <i>Fibrobacter</i>, <i>Treponema</i>, <i>Akkermansia</i>, <i>Blautia</i>, <i>Clostridium sensu stricto 1</i>, <i>Turicibacter</i>, <i>Subdoligranulum</i>, the <i>[Eubacterium] siraeum group</i>, and <i>dgA 11 gut groups</i> were positively correlated with IMF content (<i>p</i> < 0.05), whereas those of <i>Bacillus</i>, the <i>Lachnospiraceae NK4A136 group</i>, <i>Streptococcus</i>, <i>Roseburia</i>, <i>Solobacterium</i>, <i>Veillonella</i>, <i>Lactobacillus</i>, the <i>Rikenellaceae RC9 gut group</i>, <i>Anaerovibrio</i>, and the <i>Lachnospiraceae AC2044 group</i> were negatively associated with IMF content (<i>p</i> < 0.05). Employing PICRUSt2 for predicting intergenic metabolic pathways that differ among intestinal microbial communities revealed that within the 95% confidence interval the colonic microbiome was enriched with the most metabolic pathways, including those related to lipid metabolism. The diversity results, bacterial genus distributions, and metabolic pathway disparities revealed the colonic segment as an influential region for IMF deposition.https://www.mdpi.com/2076-2615/14/21/3123Min pig16S rRNA sequencinggut microbesIMF |
| spellingShingle | Long Jin Ke Li Zhimin Li Xuankai Huang Li Wang Xibiao Wang Shengwei Di Shiquan Cui Yuan Xu Investigation into Critical Gut Microbes Influencing Intramuscular Fat Deposition in Min Pigs Animals Min pig 16S rRNA sequencing gut microbes IMF |
| title | Investigation into Critical Gut Microbes Influencing Intramuscular Fat Deposition in Min Pigs |
| title_full | Investigation into Critical Gut Microbes Influencing Intramuscular Fat Deposition in Min Pigs |
| title_fullStr | Investigation into Critical Gut Microbes Influencing Intramuscular Fat Deposition in Min Pigs |
| title_full_unstemmed | Investigation into Critical Gut Microbes Influencing Intramuscular Fat Deposition in Min Pigs |
| title_short | Investigation into Critical Gut Microbes Influencing Intramuscular Fat Deposition in Min Pigs |
| title_sort | investigation into critical gut microbes influencing intramuscular fat deposition in min pigs |
| topic | Min pig 16S rRNA sequencing gut microbes IMF |
| url | https://www.mdpi.com/2076-2615/14/21/3123 |
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