Sex similarities and differences in the reverse and anti-remodeling effect of pressure unloading therapy in a rat model of aortic banding and debanding
Background: Investigating the effect of sex on pressure unloading therapy in a clinical scenario is limited by several non-standardized factors. Hence, we sought to study sex-related similarities and differences under laboratory conditions. Methods: Pressure overload was induced in male and female...
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2024-12-01
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| Series: | Cardiologia Hungarica |
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| Online Access: | https://cardiologia.hungarica.eu/archive/2024-issues/2024-6-contents/sex-similarities-and-differences-in-the-reverse-and-anti-remodeling-effect-of-pressure-unloading-therapy-in-a-rat-model-of-aortic-banding-and-debanding?article=open |
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| author | Tímea Bálint Mihály Ruppert Bálint András Barta Korkmaz-Icöz Sevil Loganathan Sivakkanan Attila Oláh Sayour Alex Ali Kálmán Benke Dávid Nagy Karck Matthias Oliver Schilling Béla Merkely Gábor Szabó |
| author_facet | Tímea Bálint Mihály Ruppert Bálint András Barta Korkmaz-Icöz Sevil Loganathan Sivakkanan Attila Oláh Sayour Alex Ali Kálmán Benke Dávid Nagy Karck Matthias Oliver Schilling Béla Merkely Gábor Szabó |
| author_sort | Tímea Bálint |
| collection | DOAJ |
| description | Background: Investigating the effect of sex on pressure unloading therapy in a clinical scenario is limited by several non-standardized factors. Hence, we sought to study sex-related similarities and differences under laboratory conditions.
Methods: Pressure overload was induced in male and female rats by aortic banding (AB) for 6 and 12 weeks. Age-matched sham operated animals served as controls. Pressure unloading was performed by aortic debanding at week 6. Different aspects of myocardial remodeling were characterized by echocardiography, pressure-volume analysis, histology, qRT-PCR and explorative proteomics.
Results: Hypertrophy, increased fetal gene expression, interstitial fibrosis, and prolonged active relaxation were noted in the AB groups at week 6 in both sexes. However, decompensation of systolic function and further deterioration of diastolic function only occurred in male AB rats at week 12. AB induced similar proteomic alterations in both sexes at week 6, while characteristic differences were found at week 12. After debanding, regression of hypertrophy and recovery of diastolic function took place to a similar extent in both sexes. Nevertheless, fibrosis, transcription of β-to-α myosin-heavy chain ratio, and myocardial proteomic alterations were reduced to a greater degree in females compared to males. Pressure unloading exposes a more pronounced anti-remodeling effect on the functional level in males, which is attributed to the more progressive functional deterioration in AB animals.
Conclusions: Pressure unloading exposes reverse and anti-remodeling properties in both sexes. Female sex is associated with greater reversibility of fibrosis, fetal gene expression, and proteomic alterations. Nevertheless, pressure unloading exposes a more pronounced anti-remodeling effect on the functional level in males. |
| format | Article |
| id | doaj-art-f701a5ccfc9948fbb9a2d365aea8ed47 |
| institution | Kabale University |
| issn | 0133-5596 1588-0230 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | Promenade Kft |
| record_format | Article |
| series | Cardiologia Hungarica |
| spelling | doaj-art-f701a5ccfc9948fbb9a2d365aea8ed472025-01-06T15:50:12ZengPromenade KftCardiologia Hungarica0133-55961588-02302024-12-0154646147810.26430/CHUNGARICA.2024.54.6.461Sex similarities and differences in the reverse and anti-remodeling effect of pressure unloading therapy in a rat model of aortic banding and debandingTímea Bálint0Mihály Ruppert1Bálint András Barta2Korkmaz-Icöz Sevil3Loganathan Sivakkanan4Attila Oláh5Sayour Alex Ali6Kálmán Benke7Dávid Nagy8Karck Matthias9Oliver Schilling10Béla Merkely11Gábor Szabó12Semmelweis Egyetem, Városmajori Szív- és Érgyógyászati Klinika, Kísérletes Kardiológiai és Sebészeti Műtéttani Tanszék, BudapestSemmelweis Egyetem, Városmajori Szív- és Érgyógyászati Klinika, Kísérletes Kardiológiai és Sebészeti Műtéttani Tanszék, BudapestSemmelweis Egyetem, Városmajori Szív- és Érgyógyászati Klinika, Kísérletes Kardiológiai és Sebészeti Műtéttani Tanszék, Budapest, Semmelweis Egyetem, Városmajori Szív- és Érgyógyászati Klinika, Budapest, Albert Ludwig Egyetem, Sebészeti Patológiai Intézet, Freiburg, Németország, Albert Ludwig Egyetem, Biológiai Kar, Freiburg, NémetországRuprecht–Karl Egyetem, Szívsebészeti Klinika, Heidelberg, NémetországRuprecht–Karl Egyetem, Szívsebészeti Klinika, Heidelberg, NémetországSemmelweis Egyetem, Városmajori Szív- és Érgyógyászati Klinika, Kísérletes Kardiológiai és Sebészeti Műtéttani Tanszék, BudapestSemmelweis Egyetem, Városmajori Szív- és Érgyógyászati Klinika, Kísérletes Kardiológiai és Sebészeti Műtéttani Tanszék, BudapestSemmelweis Egyetem, Városmajori Szív- és Érgyógyászati Klinika, Budapest, Department of Cardiac Surgery, University Hospital Halle (Saale), Szívsebészeti Klinika Halle, NémetországSemmelweis Egyetem, Városmajori Szív- és Érgyógyászati Klinika, Kísérletes Kardiológiai és Sebészeti Műtéttani Tanszék, BudapestRuprecht–Karl Egyetem, Szívsebészeti Klinika, Heidelberg, NémetországAlbert Ludwig Egyetem, Sebészeti Patológiai Intézet, Freiburg, NémetországSemmelweis Egyetem, Városmajori Szív- és Érgyógyászati Klinika, BudapestRuprecht–Karl Egyetem, Szívsebészeti Klinika, Heidelberg, Németország, Department of Cardiac Surgery, University Hospital Halle (Saale), Szívsebészeti Klinika Halle, Németország Background: Investigating the effect of sex on pressure unloading therapy in a clinical scenario is limited by several non-standardized factors. Hence, we sought to study sex-related similarities and differences under laboratory conditions. Methods: Pressure overload was induced in male and female rats by aortic banding (AB) for 6 and 12 weeks. Age-matched sham operated animals served as controls. Pressure unloading was performed by aortic debanding at week 6. Different aspects of myocardial remodeling were characterized by echocardiography, pressure-volume analysis, histology, qRT-PCR and explorative proteomics. Results: Hypertrophy, increased fetal gene expression, interstitial fibrosis, and prolonged active relaxation were noted in the AB groups at week 6 in both sexes. However, decompensation of systolic function and further deterioration of diastolic function only occurred in male AB rats at week 12. AB induced similar proteomic alterations in both sexes at week 6, while characteristic differences were found at week 12. After debanding, regression of hypertrophy and recovery of diastolic function took place to a similar extent in both sexes. Nevertheless, fibrosis, transcription of β-to-α myosin-heavy chain ratio, and myocardial proteomic alterations were reduced to a greater degree in females compared to males. Pressure unloading exposes a more pronounced anti-remodeling effect on the functional level in males, which is attributed to the more progressive functional deterioration in AB animals. Conclusions: Pressure unloading exposes reverse and anti-remodeling properties in both sexes. Female sex is associated with greater reversibility of fibrosis, fetal gene expression, and proteomic alterations. Nevertheless, pressure unloading exposes a more pronounced anti-remodeling effect on the functional level in males.https://cardiologia.hungarica.eu/archive/2024-issues/2024-6-contents/sex-similarities-and-differences-in-the-reverse-and-anti-remodeling-effect-of-pressure-unloading-therapy-in-a-rat-model-of-aortic-banding-and-debanding?article=openmyocardial hypertrophysex differencesproteomics |
| spellingShingle | Tímea Bálint Mihály Ruppert Bálint András Barta Korkmaz-Icöz Sevil Loganathan Sivakkanan Attila Oláh Sayour Alex Ali Kálmán Benke Dávid Nagy Karck Matthias Oliver Schilling Béla Merkely Gábor Szabó Sex similarities and differences in the reverse and anti-remodeling effect of pressure unloading therapy in a rat model of aortic banding and debanding Cardiologia Hungarica myocardial hypertrophy sex differences proteomics |
| title | Sex similarities and differences in the reverse and anti-remodeling effect of pressure unloading therapy in a rat model of aortic banding and debanding |
| title_full | Sex similarities and differences in the reverse and anti-remodeling effect of pressure unloading therapy in a rat model of aortic banding and debanding |
| title_fullStr | Sex similarities and differences in the reverse and anti-remodeling effect of pressure unloading therapy in a rat model of aortic banding and debanding |
| title_full_unstemmed | Sex similarities and differences in the reverse and anti-remodeling effect of pressure unloading therapy in a rat model of aortic banding and debanding |
| title_short | Sex similarities and differences in the reverse and anti-remodeling effect of pressure unloading therapy in a rat model of aortic banding and debanding |
| title_sort | sex similarities and differences in the reverse and anti remodeling effect of pressure unloading therapy in a rat model of aortic banding and debanding |
| topic | myocardial hypertrophy sex differences proteomics |
| url | https://cardiologia.hungarica.eu/archive/2024-issues/2024-6-contents/sex-similarities-and-differences-in-the-reverse-and-anti-remodeling-effect-of-pressure-unloading-therapy-in-a-rat-model-of-aortic-banding-and-debanding?article=open |
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