EFFECT OF COLLOIDAL SOLUTIONS OF METAL NANOPARTICLES AND LASER IRRADIATION ON BIOLOGICAL ACTIVITY OF THE EDIBLE MEDICINAL MACROFUNGUS Pleurotus eryngii (PLEUROTACEAE, AGARICALES) IN VITRO
Aim. The goal of our work was to study the effect of colloidal solutions of metal nanoparticles (NPs) on the synthesis of mycelial mass, polysaccharides, phenolic compounds, and the antioxidant activity of the edible medicinal macrofungus Pleurotus eryngii, as well as the effects of photocatalytic a...
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National Academy of Sciences of Ukraine, Palladin Institute of Biochemistry
2024-12-01
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| Series: | Biotechnologia Acta |
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| Online Access: | https://biotechnology.kiev.ua/images/BTA/2024/6_2024/Mykchaylova_6_2024.pdf |
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| author | O.B. Mykchaylova A.M. Negriyko K.G. Lopatko N.L. Poyedinok |
| author_facet | O.B. Mykchaylova A.M. Negriyko K.G. Lopatko N.L. Poyedinok |
| author_sort | O.B. Mykchaylova |
| collection | DOAJ |
| description | Aim. The goal of our work was to study the effect of colloidal solutions of metal nanoparticles (NPs) on the synthesis of mycelial mass, polysaccharides, phenolic compounds, and the antioxidant activity of the edible medicinal macrofungus Pleurotus eryngii, as well as the effects of photocatalytic activity of NPs after exposure to low-intensity laser radiation under submerged cultivation conditions.
Methods. Traditional mycological methods and unique photobiological methods were used. The effect of light on the biosynthetic and biological activity of P. eryngii was studied using low-intensity coherent monochromatic blue laser light (λ=488 nm). The experiment used colloidal solutions of metal nanoparticles (FeNPs, MgNPs, AgNPs) based on the method of volumetric electric spark dispersion of metals patented in Ukraine.
Results. Treatment of the inoculum with colloidal solutions of FeNPs and MgNPs increased the amount of mycelial mass of P. eryngii by 38–53%, while irradiation of the inoculum with blue laser light (λ=488 nm) in a medium with NPs increased the growth activity of the P. eryngii mycelium by 6.8‒18.2%. All nanoparticles suppressed the biosynthesis of extracellular polysaccharides. The most significant effect was observed with the addition of MgNPs – 21.4%. While the use of photoinduced nanoparticles stimulated the synthesis of extracellular polysaccharides, the most excellent effect was observed for MgNPs – 100%. The addition of all NPs to the P. eryngii inoculum reduced the amount of intracellular polysaccharides in the mycelial mass by 9.4% (MgNPs) and by 22% (AgNPs). The use of NPs photoinduced by blue laser light increased the amount of intracellular polysaccharides in the mycelial mass of P. eryngii by 28.1% (AgNPs) and by 50% (MgNPs). Treatment of the inoculum with colloidal solutions of AgNPs, FeNPs and MgNPs and laser light-induced nanoparticles increased the amount of phenolic compounds in the mycelial mass. The highest total phenolic content (TPC) values in ethanol extracts were recorded when using photoinduced MgNPs − 59.51±0.4 mg GAEs/g dry mass.
Conclusions.The results of the studies provided grounds to consider metal nanoparticles (FeNPs, MgNPs), and lowintensity blue laser radiation as promising regulators of the synthesis of polysaccharides and phenolic compounds in the mycelial mass of P. eryngii under submerged cultivation conditions. |
| format | Article |
| id | doaj-art-f6ebec0f385144a7a9fe3422a91ff8c3 |
| institution | Kabale University |
| issn | 2410-7751 2410-776X |
| language | English |
| publishDate | 2024-12-01 |
| publisher | National Academy of Sciences of Ukraine, Palladin Institute of Biochemistry |
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| series | Biotechnologia Acta |
| spelling | doaj-art-f6ebec0f385144a7a9fe3422a91ff8c32025-01-10T15:41:28ZengNational Academy of Sciences of Ukraine, Palladin Institute of BiochemistryBiotechnologia Acta2410-77512410-776X2024-12-01176152710.15407/biotech17.06.015EFFECT OF COLLOIDAL SOLUTIONS OF METAL NANOPARTICLES AND LASER IRRADIATION ON BIOLOGICAL ACTIVITY OF THE EDIBLE MEDICINAL MACROFUNGUS Pleurotus eryngii (PLEUROTACEAE, AGARICALES) IN VITROO.B. Mykchaylova0A.M. Negriyko1 K.G. Lopatko2N.L. Poyedinok3Igor Sikorsky Kyiv Polytechnic Institute, Ukraine; M.G. Kholodny Institute of Botany of the National Academy of Sciences of Ukraine, KyivInstitute of Physics of the National Academy of Sciences of Ukraine, KyivNational University of Life and Environmental Sciences of the National Academy of Science of Ukraine, KyivIgor Sikorsky Kyiv Polytechnic Institute, UkraineAim. The goal of our work was to study the effect of colloidal solutions of metal nanoparticles (NPs) on the synthesis of mycelial mass, polysaccharides, phenolic compounds, and the antioxidant activity of the edible medicinal macrofungus Pleurotus eryngii, as well as the effects of photocatalytic activity of NPs after exposure to low-intensity laser radiation under submerged cultivation conditions. Methods. Traditional mycological methods and unique photobiological methods were used. The effect of light on the biosynthetic and biological activity of P. eryngii was studied using low-intensity coherent monochromatic blue laser light (λ=488 nm). The experiment used colloidal solutions of metal nanoparticles (FeNPs, MgNPs, AgNPs) based on the method of volumetric electric spark dispersion of metals patented in Ukraine. Results. Treatment of the inoculum with colloidal solutions of FeNPs and MgNPs increased the amount of mycelial mass of P. eryngii by 38–53%, while irradiation of the inoculum with blue laser light (λ=488 nm) in a medium with NPs increased the growth activity of the P. eryngii mycelium by 6.8‒18.2%. All nanoparticles suppressed the biosynthesis of extracellular polysaccharides. The most significant effect was observed with the addition of MgNPs – 21.4%. While the use of photoinduced nanoparticles stimulated the synthesis of extracellular polysaccharides, the most excellent effect was observed for MgNPs – 100%. The addition of all NPs to the P. eryngii inoculum reduced the amount of intracellular polysaccharides in the mycelial mass by 9.4% (MgNPs) and by 22% (AgNPs). The use of NPs photoinduced by blue laser light increased the amount of intracellular polysaccharides in the mycelial mass of P. eryngii by 28.1% (AgNPs) and by 50% (MgNPs). Treatment of the inoculum with colloidal solutions of AgNPs, FeNPs and MgNPs and laser light-induced nanoparticles increased the amount of phenolic compounds in the mycelial mass. The highest total phenolic content (TPC) values in ethanol extracts were recorded when using photoinduced MgNPs − 59.51±0.4 mg GAEs/g dry mass. Conclusions.The results of the studies provided grounds to consider metal nanoparticles (FeNPs, MgNPs), and lowintensity blue laser radiation as promising regulators of the synthesis of polysaccharides and phenolic compounds in the mycelial mass of P. eryngii under submerged cultivation conditions.https://biotechnology.kiev.ua/images/BTA/2024/6_2024/Mykchaylova_6_2024.pdfcolloidal solutions nanoparticles metalslasermycelial masspolysaccharidestotal phenol compoundsantioxidant activity. |
| spellingShingle | O.B. Mykchaylova A.M. Negriyko K.G. Lopatko N.L. Poyedinok EFFECT OF COLLOIDAL SOLUTIONS OF METAL NANOPARTICLES AND LASER IRRADIATION ON BIOLOGICAL ACTIVITY OF THE EDIBLE MEDICINAL MACROFUNGUS Pleurotus eryngii (PLEUROTACEAE, AGARICALES) IN VITRO Biotechnologia Acta colloidal solutions nanoparticles metals laser mycelial mass polysaccharides total phenol compounds antioxidant activity. |
| title | EFFECT OF COLLOIDAL SOLUTIONS OF METAL NANOPARTICLES AND LASER IRRADIATION ON BIOLOGICAL ACTIVITY OF THE EDIBLE MEDICINAL MACROFUNGUS Pleurotus eryngii (PLEUROTACEAE, AGARICALES) IN VITRO |
| title_full | EFFECT OF COLLOIDAL SOLUTIONS OF METAL NANOPARTICLES AND LASER IRRADIATION ON BIOLOGICAL ACTIVITY OF THE EDIBLE MEDICINAL MACROFUNGUS Pleurotus eryngii (PLEUROTACEAE, AGARICALES) IN VITRO |
| title_fullStr | EFFECT OF COLLOIDAL SOLUTIONS OF METAL NANOPARTICLES AND LASER IRRADIATION ON BIOLOGICAL ACTIVITY OF THE EDIBLE MEDICINAL MACROFUNGUS Pleurotus eryngii (PLEUROTACEAE, AGARICALES) IN VITRO |
| title_full_unstemmed | EFFECT OF COLLOIDAL SOLUTIONS OF METAL NANOPARTICLES AND LASER IRRADIATION ON BIOLOGICAL ACTIVITY OF THE EDIBLE MEDICINAL MACROFUNGUS Pleurotus eryngii (PLEUROTACEAE, AGARICALES) IN VITRO |
| title_short | EFFECT OF COLLOIDAL SOLUTIONS OF METAL NANOPARTICLES AND LASER IRRADIATION ON BIOLOGICAL ACTIVITY OF THE EDIBLE MEDICINAL MACROFUNGUS Pleurotus eryngii (PLEUROTACEAE, AGARICALES) IN VITRO |
| title_sort | effect of colloidal solutions of metal nanoparticles and laser irradiation on biological activity of the edible medicinal macrofungus pleurotus eryngii pleurotaceae agaricales in vitro |
| topic | colloidal solutions nanoparticles metals laser mycelial mass polysaccharides total phenol compounds antioxidant activity. |
| url | https://biotechnology.kiev.ua/images/BTA/2024/6_2024/Mykchaylova_6_2024.pdf |
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