Co-Infection Dynamics of Baculovirus Penaei (BP–PvSNPV) in <i>Penaeus vannamei</i> Across Latin America
Baculovirus penaei (BP) is an enteric virus infecting the hepatopancreas and anterior midgut of shrimp, particularly affecting early developmental stages and contributing to hatchery losses. While BP’s role in co-infections is increasingly recognized, its impact on later life stages remains unclear....
Saved in:
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2025-03-01
|
| Series: | Viruses |
| Subjects: | |
| Online Access: | https://www.mdpi.com/1999-4915/17/3/374 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1849340200135688192 |
|---|---|
| author | Pablo Intriago Bolivar Montiel Mauricio Valarezo Nicole Cercado Alejandra Montenegro María Mercedes Vásquez Melany del Barco Yamilis Cataño |
| author_facet | Pablo Intriago Bolivar Montiel Mauricio Valarezo Nicole Cercado Alejandra Montenegro María Mercedes Vásquez Melany del Barco Yamilis Cataño |
| author_sort | Pablo Intriago |
| collection | DOAJ |
| description | Baculovirus penaei (BP) is an enteric virus infecting the hepatopancreas and anterior midgut of shrimp, particularly affecting early developmental stages and contributing to hatchery losses. While BP’s role in co-infections is increasingly recognized, its impact on later life stages remains unclear. Despite advancements in molecular diagnostics, its high genetic diversity complicates reliable detection, often leading to discrepancies between PCR results and histological observations of occlusion bodies. This study evaluated seven primer pairs for BP detection in <i>Penaeus vannamei</i>. Among histologically confirmed cases, only 6% tested positive with the BPA/BPF primer and 3% with BPA/BPB, while the remaining primers failed to amplify BP, highlighting significant diagnostic limitations. Histopathology revealed bacterial co-infections alongside BP, with advanced cases showing intranuclear occlusion bodies, hepatopancreatic necrosis, and epithelial detachment. These findings underscore the urgent need for improved molecular diagnostics to accurately assess BP prevalence, its role in co-infections, and its overall impact on shrimp health in Latin America. Further research is essential to refine detection methods and determine BP’s pathogenic significance beyond early developmental stages. |
| format | Article |
| id | doaj-art-f6b407a66d2a4a8cbf5c4b8d9f6987ed |
| institution | Kabale University |
| issn | 1999-4915 |
| language | English |
| publishDate | 2025-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Viruses |
| spelling | doaj-art-f6b407a66d2a4a8cbf5c4b8d9f6987ed2025-08-20T03:43:57ZengMDPI AGViruses1999-49152025-03-0117337410.3390/v17030374Co-Infection Dynamics of Baculovirus Penaei (BP–PvSNPV) in <i>Penaeus vannamei</i> Across Latin AmericaPablo Intriago0Bolivar Montiel1Mauricio Valarezo2Nicole Cercado3Alejandra Montenegro4María Mercedes Vásquez5Melany del Barco6Yamilis Cataño7South Florida Farming Corp, 13811 Old Sheridan St, Southwest Ranches, FL 33330, USASouth Florida Farming Lab, Av. Miguel Yunez, Km 14.5 Via A Samborondón, Almax 3 Etapa 1-Lote 3 Bodega 2, Samborondón, Guayas, EcuadorSouth Florida Farming Lab, Av. Miguel Yunez, Km 14.5 Via A Samborondón, Almax 3 Etapa 1-Lote 3 Bodega 2, Samborondón, Guayas, EcuadorSouth Florida Farming Lab, Av. Miguel Yunez, Km 14.5 Via A Samborondón, Almax 3 Etapa 1-Lote 3 Bodega 2, Samborondón, Guayas, EcuadorSouth Florida Farming Lab, Av. Miguel Yunez, Km 14.5 Via A Samborondón, Almax 3 Etapa 1-Lote 3 Bodega 2, Samborondón, Guayas, EcuadorSouth Florida Farming Lab, Av. Miguel Yunez, Km 14.5 Via A Samborondón, Almax 3 Etapa 1-Lote 3 Bodega 2, Samborondón, Guayas, EcuadorSouth Florida Farming Lab, Av. Miguel Yunez, Km 14.5 Via A Samborondón, Almax 3 Etapa 1-Lote 3 Bodega 2, Samborondón, Guayas, EcuadorOcéanos S.A., Centro de Producción Laboratorio, Coveñas, Sucre, ColombiaBaculovirus penaei (BP) is an enteric virus infecting the hepatopancreas and anterior midgut of shrimp, particularly affecting early developmental stages and contributing to hatchery losses. While BP’s role in co-infections is increasingly recognized, its impact on later life stages remains unclear. Despite advancements in molecular diagnostics, its high genetic diversity complicates reliable detection, often leading to discrepancies between PCR results and histological observations of occlusion bodies. This study evaluated seven primer pairs for BP detection in <i>Penaeus vannamei</i>. Among histologically confirmed cases, only 6% tested positive with the BPA/BPF primer and 3% with BPA/BPB, while the remaining primers failed to amplify BP, highlighting significant diagnostic limitations. Histopathology revealed bacterial co-infections alongside BP, with advanced cases showing intranuclear occlusion bodies, hepatopancreatic necrosis, and epithelial detachment. These findings underscore the urgent need for improved molecular diagnostics to accurately assess BP prevalence, its role in co-infections, and its overall impact on shrimp health in Latin America. Further research is essential to refine detection methods and determine BP’s pathogenic significance beyond early developmental stages.https://www.mdpi.com/1999-4915/17/3/374<i>Penaeus vannamei</i>—PvBaculovirus penaei-single enveloped nucleopolyhedron virus (BP—PvSNPV)Hepatopancreas—HPWenzhou shrimp virus 8—WzSV8Hepanhamaparvovirus—DHPVPostlarvae—PL |
| spellingShingle | Pablo Intriago Bolivar Montiel Mauricio Valarezo Nicole Cercado Alejandra Montenegro María Mercedes Vásquez Melany del Barco Yamilis Cataño Co-Infection Dynamics of Baculovirus Penaei (BP–PvSNPV) in <i>Penaeus vannamei</i> Across Latin America Viruses <i>Penaeus vannamei</i>—Pv Baculovirus penaei-single enveloped nucleopolyhedron virus (BP—PvSNPV) Hepatopancreas—HP Wenzhou shrimp virus 8—WzSV8 Hepanhamaparvovirus—DHPV Postlarvae—PL |
| title | Co-Infection Dynamics of Baculovirus Penaei (BP–PvSNPV) in <i>Penaeus vannamei</i> Across Latin America |
| title_full | Co-Infection Dynamics of Baculovirus Penaei (BP–PvSNPV) in <i>Penaeus vannamei</i> Across Latin America |
| title_fullStr | Co-Infection Dynamics of Baculovirus Penaei (BP–PvSNPV) in <i>Penaeus vannamei</i> Across Latin America |
| title_full_unstemmed | Co-Infection Dynamics of Baculovirus Penaei (BP–PvSNPV) in <i>Penaeus vannamei</i> Across Latin America |
| title_short | Co-Infection Dynamics of Baculovirus Penaei (BP–PvSNPV) in <i>Penaeus vannamei</i> Across Latin America |
| title_sort | co infection dynamics of baculovirus penaei bp pvsnpv in i penaeus vannamei i across latin america |
| topic | <i>Penaeus vannamei</i>—Pv Baculovirus penaei-single enveloped nucleopolyhedron virus (BP—PvSNPV) Hepatopancreas—HP Wenzhou shrimp virus 8—WzSV8 Hepanhamaparvovirus—DHPV Postlarvae—PL |
| url | https://www.mdpi.com/1999-4915/17/3/374 |
| work_keys_str_mv | AT pablointriago coinfectiondynamicsofbaculoviruspenaeibppvsnpvinipenaeusvannameiiacrosslatinamerica AT bolivarmontiel coinfectiondynamicsofbaculoviruspenaeibppvsnpvinipenaeusvannameiiacrosslatinamerica AT mauriciovalarezo coinfectiondynamicsofbaculoviruspenaeibppvsnpvinipenaeusvannameiiacrosslatinamerica AT nicolecercado coinfectiondynamicsofbaculoviruspenaeibppvsnpvinipenaeusvannameiiacrosslatinamerica AT alejandramontenegro coinfectiondynamicsofbaculoviruspenaeibppvsnpvinipenaeusvannameiiacrosslatinamerica AT mariamercedesvasquez coinfectiondynamicsofbaculoviruspenaeibppvsnpvinipenaeusvannameiiacrosslatinamerica AT melanydelbarco coinfectiondynamicsofbaculoviruspenaeibppvsnpvinipenaeusvannameiiacrosslatinamerica AT yamiliscatano coinfectiondynamicsofbaculoviruspenaeibppvsnpvinipenaeusvannameiiacrosslatinamerica |