EVALUATION OF MEMBRANE INTEGRITY OF BULL FROZENTHAWED SPERM USING WATER AND HYPO OSMOTIC SWELLING TEST
Assessment of the sperm membrane functional status appears to be a significant marker for the fertilizing capacity of spermatozoa. The hypo osmotic swelling test (HOST) is one of the best methods to evaluate sperm membrane integrity. In the current study, we used DW and hypo osmotic solutions of 50...
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| Main Authors: | , |
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| Format: | Article |
| Language: | English |
| Published: |
College of Veterinary Medicine, University of Basrah, Iraq
2017-12-01
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| Series: | Basrah Journal of Veterinary Research |
| Subjects: | |
| Online Access: | https://bjvr.uobasrah.edu.iq/article_143539_b8fe656416c5fab8eee8f225ed1e9882.pdf |
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| Summary: | Assessment of the sperm membrane functional status appears to be a significant marker
for the fertilizing capacity of spermatozoa. The hypo osmotic swelling test (HOST) is one of
the best methods to evaluate sperm membrane integrity. In the current study, we used DW
and hypo osmotic solutions of 50 and 100mOsm/l of dextrose/NaCl, NaCl, sucrose and
fructose. Based on the results, Among the dextrose/NaCl, NaCl, sucrose, and fructose
solutions and DW, Maximum numbers of swollen of bull frozen-thawed spermatozoa were
observed with DW and dextrose/NaCl solution at 50 mOsm with average response by
61.20±8.677 and 47.90±10.181 respectively. The HOST response at 3 and 60 min for all of
solutions were positively correlated to each other and there was no significant difference
between the responses to the HOST at 3 and 60 min after incubation in all of solutions. The
significant correlation was observed between motility and dextrose/NaCl at 50 mOsm,
sucrose 50 and 100 mOsm, NaCl 50 mOsm and DW. The high relationship was between
motility and DW and dextrose/NaCl at 50 mosm. There was no significant correlation
between DW and all of hypoosmotic solutions with staining of the spermatozoa by
eosin/nigrosin. In conclusion, the water test can be efficiently used for the evaluation of the
functional integrity of the plasma membrane of bull frozen-thawed spermatozoa. The
hypoosmular solution of dextrose/NaCl at 50 mOsm is a good medium to evaluate bull frozen
spermatozoa. The used of HOST and motility are better tests to evaluate bull frozen thawed
sperm than eosin-nigrosin. The short HOST procedure (3 min) is suitable method for
evaluating of membrane integrity of bull frozen/thawed spermatozoa |
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| ISSN: | 1813-8497 2410-8456 |