Biodistribution of intravenously delivered PEGylated carbon nanotubes to the rat brain cortex
Polyethylene glycol-functionalized single-walled carbon nanotubes (SWCNT-PEG) have been studied for many biomedical applications because of their unique physicochemical properties. Due to their reduced size and high stability in physiological media, SWCNT-PEG are candidates for crossing the blood–br...
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Frontiers Media S.A.
2025-01-01
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| Series: | Frontiers in Carbon |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/frcrb.2024.1363919/full |
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| author | Gisele Eva Bruch Gisele Eva Bruch Lidiane Dal Bosco Lidiane Dal Bosco Arthur P. Cordeiro Marcos F. Cordeiro Sangram K. Sahoo Carolina Peixoto Marta C. Klosterhoff Luis Alberto Romano Cristiano Fantini Adelina P. Santos Daniela M. Barros |
| author_facet | Gisele Eva Bruch Gisele Eva Bruch Lidiane Dal Bosco Lidiane Dal Bosco Arthur P. Cordeiro Marcos F. Cordeiro Sangram K. Sahoo Carolina Peixoto Marta C. Klosterhoff Luis Alberto Romano Cristiano Fantini Adelina P. Santos Daniela M. Barros |
| author_sort | Gisele Eva Bruch |
| collection | DOAJ |
| description | Polyethylene glycol-functionalized single-walled carbon nanotubes (SWCNT-PEG) have been studied for many biomedical applications because of their unique physicochemical properties. Due to their reduced size and high stability in physiological media, SWCNT-PEG are candidates for crossing the blood–brain barrier (BBB), with potential use in treating central nervous system diseases that are currently unresponsive to pharmacological interventions because of the tightly regulated permeability of the BBB. In this study, we investigated the biodistribution of intravenously delivered SWCNT-PEG using Raman spectroscopy, as well as possible toxicological outcomes using morphological, histological, biochemical, and behavioral analyses. SWCNT-PEG were identified in the brain cortex, blood, spleen, and liver of rats. Biochemical and histological analyses did not reveal toxic effects in rats 24 h after SWCNT-PEG injection. Additionally, no behavioral impairments were observed in treated animals subjected to the Morris water maze task. Our preliminary experimental results clearly indicate that SWCNT-PEG were able to cross biological membranes and reach the rat brain cortex parenchyma (but not other brain structures) after systemic administration without the presence of acute toxic effects. The biodistribution of SWCNT-PEG in a specific region of the brain tissue encourages further studies regarding the application of SWCNTs in neuroscience. |
| format | Article |
| id | doaj-art-f69e149d5aba4ddb85957f08269d62b7 |
| institution | Kabale University |
| issn | 2813-4192 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Carbon |
| spelling | doaj-art-f69e149d5aba4ddb85957f08269d62b72025-01-03T04:11:33ZengFrontiers Media S.A.Frontiers in Carbon2813-41922025-01-01310.3389/frcrb.2024.13639191363919Biodistribution of intravenously delivered PEGylated carbon nanotubes to the rat brain cortexGisele Eva Bruch0Gisele Eva Bruch1Lidiane Dal Bosco2Lidiane Dal Bosco3Arthur P. Cordeiro4Marcos F. Cordeiro5Sangram K. Sahoo6Carolina Peixoto7Marta C. Klosterhoff8Luis Alberto Romano9Cristiano Fantini10Adelina P. Santos11Daniela M. Barros12Laboratório de Neurociências, Instituto de Ciências Biológicas, Universidade Federal do Rio Grande (FURG), Programa de Pós-graduação em Ciências Fisiológicas - Fisiologia Animal Comparada, FURG, Rio Grande, BrazilLaboratório de Simulação Realística, Faculdade de Minas – Faminas BH, Belo Horizonte, BrazilLaboratório de Neurociências, Instituto de Ciências Biológicas, Universidade Federal do Rio Grande (FURG), Programa de Pós-graduação em Ciências Fisiológicas - Fisiologia Animal Comparada, FURG, Rio Grande, BrazilUniversidade Federal do Pampa (UNIPAMPA), Uruguaiana, BrazilLaboratório de Neurociências, Instituto de Ciências Biológicas, Universidade Federal do Rio Grande (FURG), Programa de Pós-graduação em Ciências Fisiológicas - Fisiologia Animal Comparada, FURG, Rio Grande, BrazilLaboratório de Neurociências, Instituto de Ciências Biológicas, Universidade Federal do Rio Grande (FURG), Programa de Pós-graduação em Ciências Fisiológicas - Fisiologia Animal Comparada, FURG, Rio Grande, BrazilDepartamento de Física, Universidade Federal de Minas Gerais, Belo Horizonte, BrazilLaboratório de Neurociências, Instituto de Ciências Biológicas, Universidade Federal do Rio Grande (FURG), Programa de Pós-graduação em Ciências Fisiológicas - Fisiologia Animal Comparada, FURG, Rio Grande, BrazilLaboratório de Imunologia e Patologia de Organismos Aquáticos, Instituto de Oceanografia, Universidade Federal do Rio Grande, Rio Grande, BrazilLaboratório de Imunologia e Patologia de Organismos Aquáticos, Instituto de Oceanografia, Universidade Federal do Rio Grande, Rio Grande, BrazilDepartamento de Física, Universidade Federal de Minas Gerais, Belo Horizonte, BrazilCentro de Desenvolvimento da Tecnologia Nuclear, CDTN, Belo Horizonte, BrazilLaboratório de Neurociências, Instituto de Ciências Biológicas, Universidade Federal do Rio Grande (FURG), Programa de Pós-graduação em Ciências Fisiológicas - Fisiologia Animal Comparada, FURG, Rio Grande, BrazilPolyethylene glycol-functionalized single-walled carbon nanotubes (SWCNT-PEG) have been studied for many biomedical applications because of their unique physicochemical properties. Due to their reduced size and high stability in physiological media, SWCNT-PEG are candidates for crossing the blood–brain barrier (BBB), with potential use in treating central nervous system diseases that are currently unresponsive to pharmacological interventions because of the tightly regulated permeability of the BBB. In this study, we investigated the biodistribution of intravenously delivered SWCNT-PEG using Raman spectroscopy, as well as possible toxicological outcomes using morphological, histological, biochemical, and behavioral analyses. SWCNT-PEG were identified in the brain cortex, blood, spleen, and liver of rats. Biochemical and histological analyses did not reveal toxic effects in rats 24 h after SWCNT-PEG injection. Additionally, no behavioral impairments were observed in treated animals subjected to the Morris water maze task. Our preliminary experimental results clearly indicate that SWCNT-PEG were able to cross biological membranes and reach the rat brain cortex parenchyma (but not other brain structures) after systemic administration without the presence of acute toxic effects. The biodistribution of SWCNT-PEG in a specific region of the brain tissue encourages further studies regarding the application of SWCNTs in neuroscience.https://www.frontiersin.org/articles/10.3389/frcrb.2024.1363919/fullnanomedicinecarbon nanotubespolyethylene glycolbiodistributionRaman spectroscopy |
| spellingShingle | Gisele Eva Bruch Gisele Eva Bruch Lidiane Dal Bosco Lidiane Dal Bosco Arthur P. Cordeiro Marcos F. Cordeiro Sangram K. Sahoo Carolina Peixoto Marta C. Klosterhoff Luis Alberto Romano Cristiano Fantini Adelina P. Santos Daniela M. Barros Biodistribution of intravenously delivered PEGylated carbon nanotubes to the rat brain cortex Frontiers in Carbon nanomedicine carbon nanotubes polyethylene glycol biodistribution Raman spectroscopy |
| title | Biodistribution of intravenously delivered PEGylated carbon nanotubes to the rat brain cortex |
| title_full | Biodistribution of intravenously delivered PEGylated carbon nanotubes to the rat brain cortex |
| title_fullStr | Biodistribution of intravenously delivered PEGylated carbon nanotubes to the rat brain cortex |
| title_full_unstemmed | Biodistribution of intravenously delivered PEGylated carbon nanotubes to the rat brain cortex |
| title_short | Biodistribution of intravenously delivered PEGylated carbon nanotubes to the rat brain cortex |
| title_sort | biodistribution of intravenously delivered pegylated carbon nanotubes to the rat brain cortex |
| topic | nanomedicine carbon nanotubes polyethylene glycol biodistribution Raman spectroscopy |
| url | https://www.frontiersin.org/articles/10.3389/frcrb.2024.1363919/full |
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