External nutrients fattening promotes hepatopancreases development, metabolism, nutrient deposition and flavor yield during the maturation stage in female Chinese mitten crab (Eriocheir sinensis)

The nutritive value and maturity status of hepatopancreases dominates the quality of Chinese mitten crab, Eriocheir sinensis. However, the underlying function and regulatory mechanism, as well as the external nutrient factors on the development of hepatopancreases in different growth stages of E. si...

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Main Authors: Changyou Song, Jianxiang Chen, Jiyan He, Miaomiao Xue, Pao Xu, Huiming Ding, Ningyuan Wu, Ruicheng Zhao, Gangchun Xu, Hongxia Li
Format: Article
Language:English
Published: Elsevier 2024-12-01
Series:Aquaculture Reports
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Online Access:http://www.sciencedirect.com/science/article/pii/S2352513424005167
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author Changyou Song
Jianxiang Chen
Jiyan He
Miaomiao Xue
Pao Xu
Huiming Ding
Ningyuan Wu
Ruicheng Zhao
Gangchun Xu
Hongxia Li
author_facet Changyou Song
Jianxiang Chen
Jiyan He
Miaomiao Xue
Pao Xu
Huiming Ding
Ningyuan Wu
Ruicheng Zhao
Gangchun Xu
Hongxia Li
author_sort Changyou Song
collection DOAJ
description The nutritive value and maturity status of hepatopancreases dominates the quality of Chinese mitten crab, Eriocheir sinensis. However, the underlying function and regulatory mechanism, as well as the external nutrient factors on the development of hepatopancreases in different growth stages of E. sinensis remains unclear. In this study, we first revealed the function of hepatopancreases feeding with iced trash fish (ITF) during the whole culture period; and further investigated the underlying mechanism on nutrient deposition and flavor yield feeding with formulated feed (FEED) in replacement of ITF in female E. sinensis. From the results, growth performance and metabolism indicate E. sinensis was robustly developed, and ITF promotes growth and hepatopancreases development during the maturation period. Meanwhile, activation of fatty acid and amino acid metabolism in hepatopancreases resulted in depletion of monounsaturated fatty acids (MUFAs) but promoted protein deposition during the maturation stage. Integrated analysis of transcriptome and metabolome reveal metabolism, biosynthesis, and flavor presentation were intensively active during maturation, evidenced by the expression of key genes of ALDH, TBXAs, HADHA, UPB1, CYP2, GST, and γ-GT-X1; as well as the key metabolites of GMP and IMP. Additionally, substitution of ITF with FEED further confirm full price artificial feed could meet the nutrient requirements during the maturation period, evidenced by the growth performance, fatty acid and amino acid derived nutrient and flavor yield in hepatopancreases. In conclusion, nutrient and flavor deposition was associated with the fast development of hepatopancreases, and fatten with full price compound feed contributes to the nutrient requirement and flavor yield during the maturation stage.
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spelling doaj-art-f655bd1bc2f94dec8fa819cec838215b2024-12-03T04:29:14ZengElsevierAquaculture Reports2352-51342024-12-0139102428External nutrients fattening promotes hepatopancreases development, metabolism, nutrient deposition and flavor yield during the maturation stage in female Chinese mitten crab (Eriocheir sinensis)Changyou Song0Jianxiang Chen1Jiyan He2Miaomiao Xue3Pao Xu4Huiming Ding5Ningyuan Wu6Ruicheng Zhao7Gangchun Xu8Hongxia Li9Key Laboratory of Freshwater Fisheries and Germplasm Resources Utilization, Ministry of Agricultural and Rural Affairs, Freshwater Fisheries Research Center, Chinese Academy of Fishery Sciences, Wuxi 214081, China; Wuxi Fisheries College, Nanjing Agricultural University, Wuxi 214081, China; Suzhou Yangchenghu Modern Agricultural Development Co., Ltd, Suzhou 215138, ChinaWuxi Fisheries College, Nanjing Agricultural University, Wuxi 214081, ChinaWuxi Fisheries College, Nanjing Agricultural University, Wuxi 214081, ChinaWuxi Fisheries College, Nanjing Agricultural University, Wuxi 214081, ChinaKey Laboratory of Freshwater Fisheries and Germplasm Resources Utilization, Ministry of Agricultural and Rural Affairs, Freshwater Fisheries Research Center, Chinese Academy of Fishery Sciences, Wuxi 214081, China; Wuxi Fisheries College, Nanjing Agricultural University, Wuxi 214081, ChinaSuzhou Yangchenghu Modern Agricultural Development Co., Ltd, Suzhou 215138, ChinaWuxi Fisheries College, Nanjing Agricultural University, Wuxi 214081, ChinaWuxi Fisheries College, Nanjing Agricultural University, Wuxi 214081, ChinaKey Laboratory of Freshwater Fisheries and Germplasm Resources Utilization, Ministry of Agricultural and Rural Affairs, Freshwater Fisheries Research Center, Chinese Academy of Fishery Sciences, Wuxi 214081, China; Wuxi Fisheries College, Nanjing Agricultural University, Wuxi 214081, China; Corresponding authors at: Key Laboratory of Freshwater Fisheries and Germplasm Resources Utilization, Ministry of Agricultural and Rural Affairs, Freshwater Fisheries Research Center, Chinese Academy of Fishery Sciences, Wuxi 214081, China.Key Laboratory of Freshwater Fisheries and Germplasm Resources Utilization, Ministry of Agricultural and Rural Affairs, Freshwater Fisheries Research Center, Chinese Academy of Fishery Sciences, Wuxi 214081, China; Wuxi Fisheries College, Nanjing Agricultural University, Wuxi 214081, China; Corresponding authors at: Key Laboratory of Freshwater Fisheries and Germplasm Resources Utilization, Ministry of Agricultural and Rural Affairs, Freshwater Fisheries Research Center, Chinese Academy of Fishery Sciences, Wuxi 214081, China.The nutritive value and maturity status of hepatopancreases dominates the quality of Chinese mitten crab, Eriocheir sinensis. However, the underlying function and regulatory mechanism, as well as the external nutrient factors on the development of hepatopancreases in different growth stages of E. sinensis remains unclear. In this study, we first revealed the function of hepatopancreases feeding with iced trash fish (ITF) during the whole culture period; and further investigated the underlying mechanism on nutrient deposition and flavor yield feeding with formulated feed (FEED) in replacement of ITF in female E. sinensis. From the results, growth performance and metabolism indicate E. sinensis was robustly developed, and ITF promotes growth and hepatopancreases development during the maturation period. Meanwhile, activation of fatty acid and amino acid metabolism in hepatopancreases resulted in depletion of monounsaturated fatty acids (MUFAs) but promoted protein deposition during the maturation stage. Integrated analysis of transcriptome and metabolome reveal metabolism, biosynthesis, and flavor presentation were intensively active during maturation, evidenced by the expression of key genes of ALDH, TBXAs, HADHA, UPB1, CYP2, GST, and γ-GT-X1; as well as the key metabolites of GMP and IMP. Additionally, substitution of ITF with FEED further confirm full price artificial feed could meet the nutrient requirements during the maturation period, evidenced by the growth performance, fatty acid and amino acid derived nutrient and flavor yield in hepatopancreases. In conclusion, nutrient and flavor deposition was associated with the fast development of hepatopancreases, and fatten with full price compound feed contributes to the nutrient requirement and flavor yield during the maturation stage.http://www.sciencedirect.com/science/article/pii/S2352513424005167Eriocheir sinensisFattenHepatopancreasesNutrient depositionFlavor yield
spellingShingle Changyou Song
Jianxiang Chen
Jiyan He
Miaomiao Xue
Pao Xu
Huiming Ding
Ningyuan Wu
Ruicheng Zhao
Gangchun Xu
Hongxia Li
External nutrients fattening promotes hepatopancreases development, metabolism, nutrient deposition and flavor yield during the maturation stage in female Chinese mitten crab (Eriocheir sinensis)
Aquaculture Reports
Eriocheir sinensis
Fatten
Hepatopancreases
Nutrient deposition
Flavor yield
title External nutrients fattening promotes hepatopancreases development, metabolism, nutrient deposition and flavor yield during the maturation stage in female Chinese mitten crab (Eriocheir sinensis)
title_full External nutrients fattening promotes hepatopancreases development, metabolism, nutrient deposition and flavor yield during the maturation stage in female Chinese mitten crab (Eriocheir sinensis)
title_fullStr External nutrients fattening promotes hepatopancreases development, metabolism, nutrient deposition and flavor yield during the maturation stage in female Chinese mitten crab (Eriocheir sinensis)
title_full_unstemmed External nutrients fattening promotes hepatopancreases development, metabolism, nutrient deposition and flavor yield during the maturation stage in female Chinese mitten crab (Eriocheir sinensis)
title_short External nutrients fattening promotes hepatopancreases development, metabolism, nutrient deposition and flavor yield during the maturation stage in female Chinese mitten crab (Eriocheir sinensis)
title_sort external nutrients fattening promotes hepatopancreases development metabolism nutrient deposition and flavor yield during the maturation stage in female chinese mitten crab eriocheir sinensis
topic Eriocheir sinensis
Fatten
Hepatopancreases
Nutrient deposition
Flavor yield
url http://www.sciencedirect.com/science/article/pii/S2352513424005167
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