Core–Shell PLGA Nanoparticles: In Vitro Evaluation of System Integrity
The objective of this study was to compare the properties of core–shell nanoparticles with a PLGA core and shells composed of different types of polymers, focusing on their structural integrity. The core PLGA nanoparticles were prepared either through a high-pressure homogenization–solvent evaporati...
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2024-12-01
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| Series: | Biomolecules |
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| Online Access: | https://www.mdpi.com/2218-273X/14/12/1601 |
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| author | Tatyana Kovshova Julia Malinovskaya Julia Kotova Marina Gorshkova Lyudmila Vanchugova Nadezhda Osipova Pavel Melnikov Veronika Vadekhina Alexey Nikitin Yulia Ermolenko Svetlana Gelperina |
| author_facet | Tatyana Kovshova Julia Malinovskaya Julia Kotova Marina Gorshkova Lyudmila Vanchugova Nadezhda Osipova Pavel Melnikov Veronika Vadekhina Alexey Nikitin Yulia Ermolenko Svetlana Gelperina |
| author_sort | Tatyana Kovshova |
| collection | DOAJ |
| description | The objective of this study was to compare the properties of core–shell nanoparticles with a PLGA core and shells composed of different types of polymers, focusing on their structural integrity. The core PLGA nanoparticles were prepared either through a high-pressure homogenization–solvent evaporation technique or nanoprecipitation, using poloxamer 188 (P188), a copolymer of divinyl ether with maleic anhydride (DIVEMA), and human serum albumin (HSA) as the shell-forming polymers. The shells were formed through adsorption, interfacial embedding, or conjugation. For dual fluorescent labeling, the core- and shell-forming polymers were conjugated with Cyanine5, Cyanine3, and rhodamine B. The nanoparticles had negative zeta potentials and sizes ranging from 100 to 250 nm (measured using DLS) depending on the shell structure and preparation technique. The core–shell structure was confirmed using TEM and fluorescence spectroscopy, with the appearance of FRET phenomena due to the donor–acceptor properties of the labels. All of the shells enhanced the cellular uptake of the nanoparticles in Gl261 murine glioma cells. The integrity of the core–shell structures upon their incubation with the cells was evidenced by intracellular colocalization of the fluorescent labels according to the Manders’ colocalization coefficients. This comprehensive approach may be useful for the selection of the optimal preparation method even at the early stages of the core–shell nanoparticle development. |
| format | Article |
| id | doaj-art-f5ffc20c20c94123887f9099e31d297a |
| institution | Kabale University |
| issn | 2218-273X |
| language | English |
| publishDate | 2024-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Biomolecules |
| spelling | doaj-art-f5ffc20c20c94123887f9099e31d297a2024-12-27T14:13:54ZengMDPI AGBiomolecules2218-273X2024-12-011412160110.3390/biom14121601Core–Shell PLGA Nanoparticles: In Vitro Evaluation of System IntegrityTatyana Kovshova0Julia Malinovskaya1Julia Kotova2Marina Gorshkova3Lyudmila Vanchugova4Nadezhda Osipova5Pavel Melnikov6Veronika Vadekhina7Alexey Nikitin8Yulia Ermolenko9Svetlana Gelperina10Faculty of Chemical and Pharmaceutical Technologies and Biomedical Preparations, D. Mendeleev University of Chemical Technology of Russia, Miusskaya pl. 9, Moscow 125047, RussiaFaculty of Chemical and Pharmaceutical Technologies and Biomedical Preparations, D. Mendeleev University of Chemical Technology of Russia, Miusskaya pl. 9, Moscow 125047, RussiaFaculty of Chemical and Pharmaceutical Technologies and Biomedical Preparations, D. Mendeleev University of Chemical Technology of Russia, Miusskaya pl. 9, Moscow 125047, RussiaLaboratory of Polyelectrolyte Chemistry and Biomedical Polymers, Topchiev Institute of Petrochemical Synthesis, Russian Academy of Sciences, Leninsky Prosp. 29, Moscow 119991, RussiaLaboratory of Polyelectrolyte Chemistry and Biomedical Polymers, Topchiev Institute of Petrochemical Synthesis, Russian Academy of Sciences, Leninsky Prosp. 29, Moscow 119991, RussiaFaculty of Chemical and Pharmaceutical Technologies and Biomedical Preparations, D. Mendeleev University of Chemical Technology of Russia, Miusskaya pl. 9, Moscow 125047, RussiaDepartment of Fundamental and Applied Neurobiology, V. Serbsky Federal Medical Research Centre of Psychiatry and Narcology of the Ministry of Health of the Russian Federation, Kropotkinskiy per. 23, Moscow 119034, RussiaDepartment of Fundamental and Applied Neurobiology, V. Serbsky Federal Medical Research Centre of Psychiatry and Narcology of the Ministry of Health of the Russian Federation, Kropotkinskiy per. 23, Moscow 119034, RussiaLaboratory of Biomedical Nanomaterials, National University of Science and Technology (MISIS), Leninsky Prosp. 4, Moscow 119049, RussiaFaculty of Chemical and Pharmaceutical Technologies and Biomedical Preparations, D. Mendeleev University of Chemical Technology of Russia, Miusskaya pl. 9, Moscow 125047, RussiaFaculty of Chemical and Pharmaceutical Technologies and Biomedical Preparations, D. Mendeleev University of Chemical Technology of Russia, Miusskaya pl. 9, Moscow 125047, RussiaThe objective of this study was to compare the properties of core–shell nanoparticles with a PLGA core and shells composed of different types of polymers, focusing on their structural integrity. The core PLGA nanoparticles were prepared either through a high-pressure homogenization–solvent evaporation technique or nanoprecipitation, using poloxamer 188 (P188), a copolymer of divinyl ether with maleic anhydride (DIVEMA), and human serum albumin (HSA) as the shell-forming polymers. The shells were formed through adsorption, interfacial embedding, or conjugation. For dual fluorescent labeling, the core- and shell-forming polymers were conjugated with Cyanine5, Cyanine3, and rhodamine B. The nanoparticles had negative zeta potentials and sizes ranging from 100 to 250 nm (measured using DLS) depending on the shell structure and preparation technique. The core–shell structure was confirmed using TEM and fluorescence spectroscopy, with the appearance of FRET phenomena due to the donor–acceptor properties of the labels. All of the shells enhanced the cellular uptake of the nanoparticles in Gl261 murine glioma cells. The integrity of the core–shell structures upon their incubation with the cells was evidenced by intracellular colocalization of the fluorescent labels according to the Manders’ colocalization coefficients. This comprehensive approach may be useful for the selection of the optimal preparation method even at the early stages of the core–shell nanoparticle development.https://www.mdpi.com/2218-273X/14/12/1601core–shell nanoparticlesPLGAHSADIVEMApoloxamer 188Gl261 cells |
| spellingShingle | Tatyana Kovshova Julia Malinovskaya Julia Kotova Marina Gorshkova Lyudmila Vanchugova Nadezhda Osipova Pavel Melnikov Veronika Vadekhina Alexey Nikitin Yulia Ermolenko Svetlana Gelperina Core–Shell PLGA Nanoparticles: In Vitro Evaluation of System Integrity Biomolecules core–shell nanoparticles PLGA HSA DIVEMA poloxamer 188 Gl261 cells |
| title | Core–Shell PLGA Nanoparticles: In Vitro Evaluation of System Integrity |
| title_full | Core–Shell PLGA Nanoparticles: In Vitro Evaluation of System Integrity |
| title_fullStr | Core–Shell PLGA Nanoparticles: In Vitro Evaluation of System Integrity |
| title_full_unstemmed | Core–Shell PLGA Nanoparticles: In Vitro Evaluation of System Integrity |
| title_short | Core–Shell PLGA Nanoparticles: In Vitro Evaluation of System Integrity |
| title_sort | core shell plga nanoparticles in vitro evaluation of system integrity |
| topic | core–shell nanoparticles PLGA HSA DIVEMA poloxamer 188 Gl261 cells |
| url | https://www.mdpi.com/2218-273X/14/12/1601 |
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