Anti-Listeria efficacy of peroxyacetic acid in bacterial buffered solution and on bell peppers
This study aims to 1) evaluate the efficacy of peroxyacetic acid (PAA) to inactivate Listeria monocytogenes in 0.1 % buffered-peptone-water; and 2) evaluate the reduction and mitigation of cross-contamination on peppers. In study-1, aliquots of 1.0-ml PAA solutions (133, 265, and 448 ppm) were added...
Saved in:
| Main Authors: | , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2024-12-01
|
| Series: | Journal of Agriculture and Food Research |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2666154324003028 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1846121287669252096 |
|---|---|
| author | Peighton Foster Corey Coe Carly Long Md Shafiul Islam Rion Gary Freshour Annette Freshour Jacek Jaczynski Kristen Matak Cangliang Shen |
| author_facet | Peighton Foster Corey Coe Carly Long Md Shafiul Islam Rion Gary Freshour Annette Freshour Jacek Jaczynski Kristen Matak Cangliang Shen |
| author_sort | Peighton Foster |
| collection | DOAJ |
| description | This study aims to 1) evaluate the efficacy of peroxyacetic acid (PAA) to inactivate Listeria monocytogenes in 0.1 % buffered-peptone-water; and 2) evaluate the reduction and mitigation of cross-contamination on peppers. In study-1, aliquots of 1.0-ml PAA solutions (133, 265, and 448 ppm) were added to the first 6 wells of 8-strip-deep well microplates. Then, 0.1 ml of serially diluted nalidixic-acid-resistant (NaL) L. monocytogenes was added and mixed immediately with a multichannel pipette. After exposure for 0, 5, 15, 30, and up to 120-s, 1 ml of 2 × D/E neutralized solution was added to terminate the reaction followed by spread-plating onto tryptic soy agar +200 ppm NaL and incubated at 35 °C for 48 h. In study-2, 5 inoculated red peppers were triple-washed with 15 uninoculated green peppers with 0, 23, 53, and 96 ppm of PAA and analyzed using MPN-method. L. monocytogenes counts were <0.3 log10 CFU/ml after exposure to 133, 265, and 448 ppm of PAA for 120, 90 and 60 s, respectively and fit the Linear and Weibull Models. Applying 23–96 ppm of PAA reduced L. monocytogenes by 2.03–3.32 log10MPN/g and transferred cross-contaminated cell counts by 2.36–3.17 log10MPN/g, with no differences (P > 0.05) compared to the water only treatment. Results suggested that applying allowable concentrations (23–96 ppm) of PAA during triple-wash does not show promising anti-Listeria activity on peppers. Future studies are needed to determine the minimum concentrations of the PAA solution required to effectively reduce and mitigate microbial cross-contamination of Listeria monocytogenes on peppers. |
| format | Article |
| id | doaj-art-f5db8053b2044f19bcdd1ba40daa18d8 |
| institution | Kabale University |
| issn | 2666-1543 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Agriculture and Food Research |
| spelling | doaj-art-f5db8053b2044f19bcdd1ba40daa18d82024-12-16T05:37:07ZengElsevierJournal of Agriculture and Food Research2666-15432024-12-0118101265Anti-Listeria efficacy of peroxyacetic acid in bacterial buffered solution and on bell peppersPeighton Foster0Corey Coe1Carly Long2Md Shafiul Islam Rion3Gary Freshour4Annette Freshour5Jacek Jaczynski6Kristen Matak7Cangliang Shen8Division of Animal and Nutritional Sciences, West Virginia University, Morgantown, WV, 26506, USADivision of Animal and Nutritional Sciences, West Virginia University, Morgantown, WV, 26506, USADivision of Animal and Nutritional Sciences, West Virginia University, Morgantown, WV, 26506, USADivision of Animal and Nutritional Sciences, West Virginia University, Morgantown, WV, 26506, USADivision of Animal and Nutritional Sciences, West Virginia University, Morgantown, WV, 26506, USADivision of Animal and Nutritional Sciences, West Virginia University, Morgantown, WV, 26506, USADivision of Animal and Nutritional Sciences, West Virginia University, Morgantown, WV, 26506, USADivision of Animal and Nutritional Sciences, West Virginia University, Morgantown, WV, 26506, USACorresponding author.; Division of Animal and Nutritional Sciences, West Virginia University, Morgantown, WV, 26506, USAThis study aims to 1) evaluate the efficacy of peroxyacetic acid (PAA) to inactivate Listeria monocytogenes in 0.1 % buffered-peptone-water; and 2) evaluate the reduction and mitigation of cross-contamination on peppers. In study-1, aliquots of 1.0-ml PAA solutions (133, 265, and 448 ppm) were added to the first 6 wells of 8-strip-deep well microplates. Then, 0.1 ml of serially diluted nalidixic-acid-resistant (NaL) L. monocytogenes was added and mixed immediately with a multichannel pipette. After exposure for 0, 5, 15, 30, and up to 120-s, 1 ml of 2 × D/E neutralized solution was added to terminate the reaction followed by spread-plating onto tryptic soy agar +200 ppm NaL and incubated at 35 °C for 48 h. In study-2, 5 inoculated red peppers were triple-washed with 15 uninoculated green peppers with 0, 23, 53, and 96 ppm of PAA and analyzed using MPN-method. L. monocytogenes counts were <0.3 log10 CFU/ml after exposure to 133, 265, and 448 ppm of PAA for 120, 90 and 60 s, respectively and fit the Linear and Weibull Models. Applying 23–96 ppm of PAA reduced L. monocytogenes by 2.03–3.32 log10MPN/g and transferred cross-contaminated cell counts by 2.36–3.17 log10MPN/g, with no differences (P > 0.05) compared to the water only treatment. Results suggested that applying allowable concentrations (23–96 ppm) of PAA during triple-wash does not show promising anti-Listeria activity on peppers. Future studies are needed to determine the minimum concentrations of the PAA solution required to effectively reduce and mitigate microbial cross-contamination of Listeria monocytogenes on peppers.http://www.sciencedirect.com/science/article/pii/S2666154324003028Triple-washListeria monocytogenesPeppersPeroxyacetic acid |
| spellingShingle | Peighton Foster Corey Coe Carly Long Md Shafiul Islam Rion Gary Freshour Annette Freshour Jacek Jaczynski Kristen Matak Cangliang Shen Anti-Listeria efficacy of peroxyacetic acid in bacterial buffered solution and on bell peppers Journal of Agriculture and Food Research Triple-wash Listeria monocytogenes Peppers Peroxyacetic acid |
| title | Anti-Listeria efficacy of peroxyacetic acid in bacterial buffered solution and on bell peppers |
| title_full | Anti-Listeria efficacy of peroxyacetic acid in bacterial buffered solution and on bell peppers |
| title_fullStr | Anti-Listeria efficacy of peroxyacetic acid in bacterial buffered solution and on bell peppers |
| title_full_unstemmed | Anti-Listeria efficacy of peroxyacetic acid in bacterial buffered solution and on bell peppers |
| title_short | Anti-Listeria efficacy of peroxyacetic acid in bacterial buffered solution and on bell peppers |
| title_sort | anti listeria efficacy of peroxyacetic acid in bacterial buffered solution and on bell peppers |
| topic | Triple-wash Listeria monocytogenes Peppers Peroxyacetic acid |
| url | http://www.sciencedirect.com/science/article/pii/S2666154324003028 |
| work_keys_str_mv | AT peightonfoster antilisteriaefficacyofperoxyaceticacidinbacterialbufferedsolutionandonbellpeppers AT coreycoe antilisteriaefficacyofperoxyaceticacidinbacterialbufferedsolutionandonbellpeppers AT carlylong antilisteriaefficacyofperoxyaceticacidinbacterialbufferedsolutionandonbellpeppers AT mdshafiulislamrion antilisteriaefficacyofperoxyaceticacidinbacterialbufferedsolutionandonbellpeppers AT garyfreshour antilisteriaefficacyofperoxyaceticacidinbacterialbufferedsolutionandonbellpeppers AT annettefreshour antilisteriaefficacyofperoxyaceticacidinbacterialbufferedsolutionandonbellpeppers AT jacekjaczynski antilisteriaefficacyofperoxyaceticacidinbacterialbufferedsolutionandonbellpeppers AT kristenmatak antilisteriaefficacyofperoxyaceticacidinbacterialbufferedsolutionandonbellpeppers AT cangliangshen antilisteriaefficacyofperoxyaceticacidinbacterialbufferedsolutionandonbellpeppers |