AgoArmet and AgoC002: key effector proteins in cotton aphids host adaptation
Aphids are insects that feed on phloem and introduce effector proteins into plant cells through saliva. These effector proteins are key in regulating host plant defense and enhancing aphid host adaptation. We identified these salivary proteins in the cotton aphids genome and named them AgoArmet and...
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| Format: | Article |
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Frontiers Media S.A.
2024-11-01
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| Series: | Frontiers in Plant Science |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fpls.2024.1500834/full |
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| author | Hui Xue Hui Xue Hui Xue Mengjie Yan Mengjie Yan Xiangzhen Zhu Xiangzhen Zhu Li Wang Li Wang Lizhen Chen Junyu Luo Junyu Luo Jinjie Cui Jinjie Cui Xueke Gao Xueke Gao |
| author_facet | Hui Xue Hui Xue Hui Xue Mengjie Yan Mengjie Yan Xiangzhen Zhu Xiangzhen Zhu Li Wang Li Wang Lizhen Chen Junyu Luo Junyu Luo Jinjie Cui Jinjie Cui Xueke Gao Xueke Gao |
| author_sort | Hui Xue |
| collection | DOAJ |
| description | Aphids are insects that feed on phloem and introduce effector proteins into plant cells through saliva. These effector proteins are key in regulating host plant defense and enhancing aphid host adaptation. We identified these salivary proteins in the cotton aphids genome and named them AgoArmet and AgoC002. Multiple sequence alignment, protein structure analysis, and phylogenetic analysis of these proteins with related proteins from other insects showed that AgoArmet and Armet of Aphis craccivora have high sequence identity (97%) and belong to the same evolutionary branch and that AgoC002 shares the highest sequence identity (80%) and closest evolutionary relationship with C002 of Aphis glyceins. Expression profiling of AgoArmet and AgoC002 showed that they were most highly expressed in cotton aphids during the adult-3d period. Cotton aphids transferred to zucchini leaves resulted in a significant increase in the expression of AgoArmet and AgoC002 within 48h. To investigate the functions of AgoArmet and AgoC002, we decreased the expression of these genes in cotton using virus-induced gene silencing (VIGS), which ultimately led to a 38% and 26% decrease in cotton aphids fecundity, respectively. Moreover, the reduction in AgoC002 expression resulted in a significant (24%) reduction in body weight. Taken together, our findings demonstrate that AgoArmet and AgoC002 are key effector proteins involved in cotton aphids feeding and host adaptation. |
| format | Article |
| id | doaj-art-f5c52792814e48e69c8de18a4b7b87ae |
| institution | Kabale University |
| issn | 1664-462X |
| language | English |
| publishDate | 2024-11-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Plant Science |
| spelling | doaj-art-f5c52792814e48e69c8de18a4b7b87ae2024-11-28T04:19:58ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2024-11-011510.3389/fpls.2024.15008341500834AgoArmet and AgoC002: key effector proteins in cotton aphids host adaptationHui Xue0Hui Xue1Hui Xue2Mengjie Yan3Mengjie Yan4Xiangzhen Zhu5Xiangzhen Zhu6Li Wang7Li Wang8Lizhen Chen9Junyu Luo10Junyu Luo11Jinjie Cui12Jinjie Cui13Xueke Gao14Xueke Gao15Research Base of Zhengzhou University, State Key Laboratory of Cotton Bio-breeding and Integrated Utilization, Institute of Cotton Research, Chinese Academy of Agricultural Sciences, Anyang, ChinaState Key Laboratory of Cotton Bio-Breeding and Integrated Utilization, School of Agricultural Sciences, Zhengzhou University, Zhengzhou, ChinaCollege of Plant Science and Technology, Huazhong Agricultural University, Wuhan, ChinaResearch Base of Zhengzhou University, State Key Laboratory of Cotton Bio-breeding and Integrated Utilization, Institute of Cotton Research, Chinese Academy of Agricultural Sciences, Anyang, ChinaState Key Laboratory of Cotton Bio-Breeding and Integrated Utilization, School of Agricultural Sciences, Zhengzhou University, Zhengzhou, ChinaResearch Base of Zhengzhou University, State Key Laboratory of Cotton Bio-breeding and Integrated Utilization, Institute of Cotton Research, Chinese Academy of Agricultural Sciences, Anyang, ChinaState Key Laboratory of Cotton Bio-Breeding and Integrated Utilization, School of Agricultural Sciences, Zhengzhou University, Zhengzhou, ChinaResearch Base of Zhengzhou University, State Key Laboratory of Cotton Bio-breeding and Integrated Utilization, Institute of Cotton Research, Chinese Academy of Agricultural Sciences, Anyang, ChinaState Key Laboratory of Cotton Bio-Breeding and Integrated Utilization, School of Agricultural Sciences, Zhengzhou University, Zhengzhou, ChinaCollege of Plant Science and Technology, Huazhong Agricultural University, Wuhan, ChinaResearch Base of Zhengzhou University, State Key Laboratory of Cotton Bio-breeding and Integrated Utilization, Institute of Cotton Research, Chinese Academy of Agricultural Sciences, Anyang, ChinaState Key Laboratory of Cotton Bio-Breeding and Integrated Utilization, School of Agricultural Sciences, Zhengzhou University, Zhengzhou, ChinaResearch Base of Zhengzhou University, State Key Laboratory of Cotton Bio-breeding and Integrated Utilization, Institute of Cotton Research, Chinese Academy of Agricultural Sciences, Anyang, ChinaState Key Laboratory of Cotton Bio-Breeding and Integrated Utilization, School of Agricultural Sciences, Zhengzhou University, Zhengzhou, ChinaResearch Base of Zhengzhou University, State Key Laboratory of Cotton Bio-breeding and Integrated Utilization, Institute of Cotton Research, Chinese Academy of Agricultural Sciences, Anyang, ChinaState Key Laboratory of Cotton Bio-Breeding and Integrated Utilization, School of Agricultural Sciences, Zhengzhou University, Zhengzhou, ChinaAphids are insects that feed on phloem and introduce effector proteins into plant cells through saliva. These effector proteins are key in regulating host plant defense and enhancing aphid host adaptation. We identified these salivary proteins in the cotton aphids genome and named them AgoArmet and AgoC002. Multiple sequence alignment, protein structure analysis, and phylogenetic analysis of these proteins with related proteins from other insects showed that AgoArmet and Armet of Aphis craccivora have high sequence identity (97%) and belong to the same evolutionary branch and that AgoC002 shares the highest sequence identity (80%) and closest evolutionary relationship with C002 of Aphis glyceins. Expression profiling of AgoArmet and AgoC002 showed that they were most highly expressed in cotton aphids during the adult-3d period. Cotton aphids transferred to zucchini leaves resulted in a significant increase in the expression of AgoArmet and AgoC002 within 48h. To investigate the functions of AgoArmet and AgoC002, we decreased the expression of these genes in cotton using virus-induced gene silencing (VIGS), which ultimately led to a 38% and 26% decrease in cotton aphids fecundity, respectively. Moreover, the reduction in AgoC002 expression resulted in a significant (24%) reduction in body weight. Taken together, our findings demonstrate that AgoArmet and AgoC002 are key effector proteins involved in cotton aphids feeding and host adaptation.https://www.frontiersin.org/articles/10.3389/fpls.2024.1500834/fullaphidsalivary proteinArmetC002plant defenseVIGS |
| spellingShingle | Hui Xue Hui Xue Hui Xue Mengjie Yan Mengjie Yan Xiangzhen Zhu Xiangzhen Zhu Li Wang Li Wang Lizhen Chen Junyu Luo Junyu Luo Jinjie Cui Jinjie Cui Xueke Gao Xueke Gao AgoArmet and AgoC002: key effector proteins in cotton aphids host adaptation Frontiers in Plant Science aphid salivary protein Armet C002 plant defense VIGS |
| title | AgoArmet and AgoC002: key effector proteins in cotton aphids host adaptation |
| title_full | AgoArmet and AgoC002: key effector proteins in cotton aphids host adaptation |
| title_fullStr | AgoArmet and AgoC002: key effector proteins in cotton aphids host adaptation |
| title_full_unstemmed | AgoArmet and AgoC002: key effector proteins in cotton aphids host adaptation |
| title_short | AgoArmet and AgoC002: key effector proteins in cotton aphids host adaptation |
| title_sort | agoarmet and agoc002 key effector proteins in cotton aphids host adaptation |
| topic | aphid salivary protein Armet C002 plant defense VIGS |
| url | https://www.frontiersin.org/articles/10.3389/fpls.2024.1500834/full |
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