Removal of BPA by Pseudomonas asiatica P1: Synergistic response mechanism of toxicity resistance and biodegradation
Bisphenol A (BPA) is a globally concerning toxic pollutant, and microbial degradation is considered an effective method to treat BPA contamination. However, the inherent microbial toxicity of BPA is often overlooked, particularly the microbial mechanisms of resistance and detoxification against BPA....
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Elsevier
2024-12-01
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| Series: | Ecotoxicology and Environmental Safety |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S0147651324014866 |
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| author | Kejian Tian Jinming Gu Yibing Wang Fenglin Zhang Dandan Zhou Qing Qiu Yue Yu Xuejian Sun Menghan Chang Xinwen Zhang Hongliang Huo |
| author_facet | Kejian Tian Jinming Gu Yibing Wang Fenglin Zhang Dandan Zhou Qing Qiu Yue Yu Xuejian Sun Menghan Chang Xinwen Zhang Hongliang Huo |
| author_sort | Kejian Tian |
| collection | DOAJ |
| description | Bisphenol A (BPA) is a globally concerning toxic pollutant, and microbial degradation is considered an effective method to treat BPA contamination. However, the inherent microbial toxicity of BPA is often overlooked, particularly the microbial mechanisms of resistance and detoxification against BPA. This study found that under the toxic stress of BPA, cbb3-type cytochrome c oxidase (cbb3-Cox) in the cells of Pseudomonas asiatica P1 (P. asiatica P1) was the first to resist the toxicity. Genes such as ccoNOQPG showed significant upregulation with an average log2FC value of 3.56. Subsequently, genes that are related to metal ion binding, transport, and DNA repair were upregulated in the middle to later phase, which enhanced the metabolic functions of the strains and induced strain mutations to assist P. asiatica P1 in resisting the BPA toxicity. Meanwhile, three potential BPA degradation genes were identified, among which sdrP1 was crucial to the BPA degradation and detoxification. After genetic recombination, sdrP1 achieved a degradation rate of 92.52 % for BPA. Furthermore, through various methods such as alkyl interactions, sdrP1 exhibited oxidation and demethylation to form lower toxic intermediate products and complete the biological detoxification of BPA. This study provides a systematic analysis of the toxicity resistance, biodegradation, and detoxification processes in bacterial BPA removal, refines the mechanism of BPA biodegradation and contributes to a more comprehensive and systematic understanding of the overall process of microbial removal of toxic pollutants. |
| format | Article |
| id | doaj-art-f5b18cfa702248189b2bfbdbf106c81c |
| institution | Kabale University |
| issn | 0147-6513 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Ecotoxicology and Environmental Safety |
| spelling | doaj-art-f5b18cfa702248189b2bfbdbf106c81c2024-12-07T08:24:42ZengElsevierEcotoxicology and Environmental Safety0147-65132024-12-01288117410Removal of BPA by Pseudomonas asiatica P1: Synergistic response mechanism of toxicity resistance and biodegradationKejian Tian0Jinming Gu1Yibing Wang2Fenglin Zhang3Dandan Zhou4Qing Qiu5Yue Yu6Xuejian Sun7Menghan Chang8Xinwen Zhang9Hongliang Huo10School of Environment, Northeast Normal University, No. 2555 Jingyue Avenue, Changchun City, Jilin Province, China; Ministry of Education, Engineering Research Center of Low-Carbon Treatment and Green Development of Polluted Water in Northeast China, ChinaSchool of Environment, Northeast Normal University, No. 2555 Jingyue Avenue, Changchun City, Jilin Province, ChinaSchool of Environment, Northeast Normal University, No. 2555 Jingyue Avenue, Changchun City, Jilin Province, ChinaSchool of Environment, Northeast Normal University, No. 2555 Jingyue Avenue, Changchun City, Jilin Province, ChinaMinistry of Education, Engineering Research Center of Low-Carbon Treatment and Green Development of Polluted Water in Northeast China, China; Engineering Lab for Water Pollution Control and Resources Recovery of Jilin Province, Changchun 130117, ChinaSchool of Environment, Northeast Normal University, No. 2555 Jingyue Avenue, Changchun City, Jilin Province, ChinaSchool of Environment, Northeast Normal University, No. 2555 Jingyue Avenue, Changchun City, Jilin Province, ChinaSchool of Environment, Northeast Normal University, No. 2555 Jingyue Avenue, Changchun City, Jilin Province, ChinaSchool of Environment, Northeast Normal University, No. 2555 Jingyue Avenue, Changchun City, Jilin Province, ChinaCollege of Pharmacy, Hainan Vocational University of Science and Technology, Haikou 571126, ChinaMinistry of Education, Engineering Research Center of Low-Carbon Treatment and Green Development of Polluted Water in Northeast China, China; Engineering Lab for Water Pollution Control and Resources Recovery of Jilin Province, Changchun 130117, China; Correspondence to: No. 2555 Jingyue Avenue, Changchun City, Jilin Province, 130117, ChinaBisphenol A (BPA) is a globally concerning toxic pollutant, and microbial degradation is considered an effective method to treat BPA contamination. However, the inherent microbial toxicity of BPA is often overlooked, particularly the microbial mechanisms of resistance and detoxification against BPA. This study found that under the toxic stress of BPA, cbb3-type cytochrome c oxidase (cbb3-Cox) in the cells of Pseudomonas asiatica P1 (P. asiatica P1) was the first to resist the toxicity. Genes such as ccoNOQPG showed significant upregulation with an average log2FC value of 3.56. Subsequently, genes that are related to metal ion binding, transport, and DNA repair were upregulated in the middle to later phase, which enhanced the metabolic functions of the strains and induced strain mutations to assist P. asiatica P1 in resisting the BPA toxicity. Meanwhile, three potential BPA degradation genes were identified, among which sdrP1 was crucial to the BPA degradation and detoxification. After genetic recombination, sdrP1 achieved a degradation rate of 92.52 % for BPA. Furthermore, through various methods such as alkyl interactions, sdrP1 exhibited oxidation and demethylation to form lower toxic intermediate products and complete the biological detoxification of BPA. This study provides a systematic analysis of the toxicity resistance, biodegradation, and detoxification processes in bacterial BPA removal, refines the mechanism of BPA biodegradation and contributes to a more comprehensive and systematic understanding of the overall process of microbial removal of toxic pollutants.http://www.sciencedirect.com/science/article/pii/S0147651324014866Bisphenol AToxicity resistanceShort chain dehydrogenaseBPA degradation genesPseudomonas asiaticaGenetic recombination |
| spellingShingle | Kejian Tian Jinming Gu Yibing Wang Fenglin Zhang Dandan Zhou Qing Qiu Yue Yu Xuejian Sun Menghan Chang Xinwen Zhang Hongliang Huo Removal of BPA by Pseudomonas asiatica P1: Synergistic response mechanism of toxicity resistance and biodegradation Ecotoxicology and Environmental Safety Bisphenol A Toxicity resistance Short chain dehydrogenase BPA degradation genes Pseudomonas asiatica Genetic recombination |
| title | Removal of BPA by Pseudomonas asiatica P1: Synergistic response mechanism of toxicity resistance and biodegradation |
| title_full | Removal of BPA by Pseudomonas asiatica P1: Synergistic response mechanism of toxicity resistance and biodegradation |
| title_fullStr | Removal of BPA by Pseudomonas asiatica P1: Synergistic response mechanism of toxicity resistance and biodegradation |
| title_full_unstemmed | Removal of BPA by Pseudomonas asiatica P1: Synergistic response mechanism of toxicity resistance and biodegradation |
| title_short | Removal of BPA by Pseudomonas asiatica P1: Synergistic response mechanism of toxicity resistance and biodegradation |
| title_sort | removal of bpa by pseudomonas asiatica p1 synergistic response mechanism of toxicity resistance and biodegradation |
| topic | Bisphenol A Toxicity resistance Short chain dehydrogenase BPA degradation genes Pseudomonas asiatica Genetic recombination |
| url | http://www.sciencedirect.com/science/article/pii/S0147651324014866 |
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