A Versatile Method to Create Antibody/Split‐Enzyme Complexes and Its Application to a Rapid, Homogeneous, and Universal Electrochemical Immunosensing System

A Versatile Method to Create Antibody/Split‐Enzyme Complexes and Its Application to a Rapid, Homogeneous, and Universal Electrochemical Immunosensing System

Abstract In this study, a versatile method is developed to create an antibody/split‐enzyme complex for use in electrochemical immunosensors. SpyCatchers are genetically fused at each terminus of flavin adenine dinucleotide‐dependent glucose dehydrogenase (FAD‐GDH) and a protease recognition sequence...

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Bibliographic Details
Main Authors: Yuka Tobita, Kensuke Hirano, Daimei Miura, Yuma Hatano, Wakako Tsugawa, Kazunori Ikebukuro, Koji Sode, Ryutaro Asano
Format: Article
Language:English
Published: Wiley-VCH 2025-01-01
Series:Advanced Sensor Research
Subjects:
C‐reactive protein
electrochemical sensors
homogeneous detection
single‐chain fragment variables
split glucose dehydrogenases
Online Access:https://doi.org/10.1002/adsr.202400112
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