Salvage pathway of vitamin B12 absorption in chickens with mutant tumor virus a receptor
The tumor virus A receptor (TVA), a member of the low-density lipoprotein receptor (LDLR) family, serves as an entry receptor for Avian Leukosis Virus (ALV) subgroups A and K, as well as a receptor for vitamin B12 bound to transcobalamin. Naturally occurring genetic variants in the TVA gene determin...
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Elsevier
2025-02-01
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| Series: | Poultry Science |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S0032579124013221 |
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| author | Yun Ji Shin Jin-Kyoo Kim Seung Je Woo Byung Chul Park Jae Yong Han |
| author_facet | Yun Ji Shin Jin-Kyoo Kim Seung Je Woo Byung Chul Park Jae Yong Han |
| author_sort | Yun Ji Shin |
| collection | DOAJ |
| description | The tumor virus A receptor (TVA), a member of the low-density lipoprotein receptor (LDLR) family, serves as an entry receptor for Avian Leukosis Virus (ALV) subgroups A and K, as well as a receptor for vitamin B12 bound to transcobalamin. Naturally occurring genetic variants in the TVA gene determine susceptibility or resistance to ALV-A and -K, but the effects of these mutated TVA on vitamin B12 uptake have not been investigated systemically. We found four TVA variants comprising the wild type (TVAWT), a single nucleotide polymorphism variant (TVASNP), and two partial deletions in the splicing branch point region (TVAR). This study investigates the relationship between the various genotypes of TVA alleles and uptake of vitamin B12 in chickens. A protein interaction model suggested that mutant TVAs (i.e., TVASNP, TVAR) may have reduced ability to take up vitamin B12 due to a disrupted LDL-A domain, a pivotal region involved in vitamin B12 uptake; however, we found no significant difference in absorption of vitamin B12 in TVAWT and TVASNP chickens, or levels of its metabolite in serum. Notably, TVAR chickens had significantly higher levels of vitamin B12 than TVAWT chickens, a finding contrary to the predicted lower uptake. Expression of vitamin B12 carrier related genes (i.e., AMN, GIF, and TCN2) in chickens showed a stepwise increase: TVAWT > TVASNP > TVAR. These results suggest a mechanism by which mutant TVA chickens with a disrupted TVA protein acquire natural resistance to ALV-A -K, with no impairment of vitamin B12 metabolism. |
| format | Article |
| id | doaj-art-f59e0b4820a84d719bd1be8667ce9bdf |
| institution | Kabale University |
| issn | 0032-5791 |
| language | English |
| publishDate | 2025-02-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Poultry Science |
| spelling | doaj-art-f59e0b4820a84d719bd1be8667ce9bdf2025-01-05T04:27:44ZengElsevierPoultry Science0032-57912025-02-011042104744Salvage pathway of vitamin B12 absorption in chickens with mutant tumor virus a receptorYun Ji Shin0Jin-Kyoo Kim1Seung Je Woo2Byung Chul Park3Jae Yong Han4Department of Agricultural Biotechnology and Research Institute of Agriculture and Life Sciences, Seoul National University, Seoul, Republic of KoreaDepartment of International Agricultural Technology & Institute of Green Bioscience and Technology, Seoul National University, Pyeongchang, Republic of KoreaDepartment of Agricultural Biotechnology and Research Institute of Agriculture and Life Sciences, Seoul National University, Seoul, Republic of KoreaDepartment of International Agricultural Technology & Institute of Green Bioscience and Technology, Seoul National University, Pyeongchang, Republic of KoreaDepartment of Agricultural Biotechnology and Research Institute of Agriculture and Life Sciences, Seoul National University, Seoul, Republic of Korea; Department of International Agricultural Technology & Institute of Green Bioscience and Technology, Seoul National University, Pyeongchang, Republic of Korea; Corresponding author.The tumor virus A receptor (TVA), a member of the low-density lipoprotein receptor (LDLR) family, serves as an entry receptor for Avian Leukosis Virus (ALV) subgroups A and K, as well as a receptor for vitamin B12 bound to transcobalamin. Naturally occurring genetic variants in the TVA gene determine susceptibility or resistance to ALV-A and -K, but the effects of these mutated TVA on vitamin B12 uptake have not been investigated systemically. We found four TVA variants comprising the wild type (TVAWT), a single nucleotide polymorphism variant (TVASNP), and two partial deletions in the splicing branch point region (TVAR). This study investigates the relationship between the various genotypes of TVA alleles and uptake of vitamin B12 in chickens. A protein interaction model suggested that mutant TVAs (i.e., TVASNP, TVAR) may have reduced ability to take up vitamin B12 due to a disrupted LDL-A domain, a pivotal region involved in vitamin B12 uptake; however, we found no significant difference in absorption of vitamin B12 in TVAWT and TVASNP chickens, or levels of its metabolite in serum. Notably, TVAR chickens had significantly higher levels of vitamin B12 than TVAWT chickens, a finding contrary to the predicted lower uptake. Expression of vitamin B12 carrier related genes (i.e., AMN, GIF, and TCN2) in chickens showed a stepwise increase: TVAWT > TVASNP > TVAR. These results suggest a mechanism by which mutant TVA chickens with a disrupted TVA protein acquire natural resistance to ALV-A -K, with no impairment of vitamin B12 metabolism.http://www.sciencedirect.com/science/article/pii/S0032579124013221Avian leukosis virus -A/-KChickenTVATranscobalamin 2Vitamin B12 |
| spellingShingle | Yun Ji Shin Jin-Kyoo Kim Seung Je Woo Byung Chul Park Jae Yong Han Salvage pathway of vitamin B12 absorption in chickens with mutant tumor virus a receptor Poultry Science Avian leukosis virus -A/-K Chicken TVA Transcobalamin 2 Vitamin B12 |
| title | Salvage pathway of vitamin B12 absorption in chickens with mutant tumor virus a receptor |
| title_full | Salvage pathway of vitamin B12 absorption in chickens with mutant tumor virus a receptor |
| title_fullStr | Salvage pathway of vitamin B12 absorption in chickens with mutant tumor virus a receptor |
| title_full_unstemmed | Salvage pathway of vitamin B12 absorption in chickens with mutant tumor virus a receptor |
| title_short | Salvage pathway of vitamin B12 absorption in chickens with mutant tumor virus a receptor |
| title_sort | salvage pathway of vitamin b12 absorption in chickens with mutant tumor virus a receptor |
| topic | Avian leukosis virus -A/-K Chicken TVA Transcobalamin 2 Vitamin B12 |
| url | http://www.sciencedirect.com/science/article/pii/S0032579124013221 |
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