Single-cell RNA sequencing identifies the expression of hemoglobin in chondrocyte cell subpopulations in osteoarthritis
Abstract In recent years, chondrocytes have been found to contain hemoglobin, which might be an alternative strategy for adapting to the hypoxic environment, while the potential mechanisms of that is still unclear. Here, we report the expression characteristics and potential associated pathways of h...
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| Format: | Article |
| Language: | English |
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BMC
2024-12-01
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| Series: | BMC Molecular and Cell Biology |
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| Online Access: | https://doi.org/10.1186/s12860-024-00519-3 |
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| author | Zhihao Zhang Ting He Hongwen Gu Yuanhang Zhao Shilei Tang Kangen Han Yin Hu Hongwei Wang Hailong Yu |
| author_facet | Zhihao Zhang Ting He Hongwen Gu Yuanhang Zhao Shilei Tang Kangen Han Yin Hu Hongwei Wang Hailong Yu |
| author_sort | Zhihao Zhang |
| collection | DOAJ |
| description | Abstract In recent years, chondrocytes have been found to contain hemoglobin, which might be an alternative strategy for adapting to the hypoxic environment, while the potential mechanisms of that is still unclear. Here, we report the expression characteristics and potential associated pathways of hemoglobin in chondrocytes using single-cell RNA sequencing (scRNA-seq). We downloaded data of normal people and patients with osteoarthritis (OA) from the Gene Expression Omnibus (GEO) database and cells are unbiased clustered based on gene expression pattern. We determined the expression levels of hemoglobin in various chondrocyte subpopulations. Meanwhile, we further explored the difference in the enriched signaling pathways and the cell-cell interaction in chondrocytes of the hemoglobin high-expression and low-expression groups. Specifically, we found that SPP1 was closely associated with the expression of hemoglobin in OA progression. Our findings provide new insights into the distribution characteristics of hemoglobin in chondrocytes and provide potential clues to the underlying role of hemoglobin in OA and the mechanisms related to that, providing potential new ideas for the treatment of OA. |
| format | Article |
| id | doaj-art-f53ed5edf1ac4f5f8c56673c1866423c |
| institution | Kabale University |
| issn | 2661-8850 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Molecular and Cell Biology |
| spelling | doaj-art-f53ed5edf1ac4f5f8c56673c1866423c2025-01-05T12:49:47ZengBMCBMC Molecular and Cell Biology2661-88502024-12-0125111310.1186/s12860-024-00519-3Single-cell RNA sequencing identifies the expression of hemoglobin in chondrocyte cell subpopulations in osteoarthritisZhihao Zhang0Ting He1Hongwen Gu2Yuanhang Zhao3Shilei Tang4Kangen Han5Yin Hu6Hongwei Wang7Hailong Yu8General Hospital of Northern Theater CommandXi’an Key Laboratory of Stem Cell and Regenerative Medicine, Institute of Medical Research, Northwestern Polytechnical UniversityGeneral Hospital of Northern Theater CommandGeneral Hospital of Northern Theater CommandGeneral Hospital of Northern Theater CommandGeneral Hospital of Northern Theater CommandGeneral Hospital of Northern Theater CommandGeneral Hospital of Northern Theater CommandGeneral Hospital of Northern Theater CommandAbstract In recent years, chondrocytes have been found to contain hemoglobin, which might be an alternative strategy for adapting to the hypoxic environment, while the potential mechanisms of that is still unclear. Here, we report the expression characteristics and potential associated pathways of hemoglobin in chondrocytes using single-cell RNA sequencing (scRNA-seq). We downloaded data of normal people and patients with osteoarthritis (OA) from the Gene Expression Omnibus (GEO) database and cells are unbiased clustered based on gene expression pattern. We determined the expression levels of hemoglobin in various chondrocyte subpopulations. Meanwhile, we further explored the difference in the enriched signaling pathways and the cell-cell interaction in chondrocytes of the hemoglobin high-expression and low-expression groups. Specifically, we found that SPP1 was closely associated with the expression of hemoglobin in OA progression. Our findings provide new insights into the distribution characteristics of hemoglobin in chondrocytes and provide potential clues to the underlying role of hemoglobin in OA and the mechanisms related to that, providing potential new ideas for the treatment of OA.https://doi.org/10.1186/s12860-024-00519-3Single-cell sequencingHemoglobinOsteoarthritisChondrocyte |
| spellingShingle | Zhihao Zhang Ting He Hongwen Gu Yuanhang Zhao Shilei Tang Kangen Han Yin Hu Hongwei Wang Hailong Yu Single-cell RNA sequencing identifies the expression of hemoglobin in chondrocyte cell subpopulations in osteoarthritis BMC Molecular and Cell Biology Single-cell sequencing Hemoglobin Osteoarthritis Chondrocyte |
| title | Single-cell RNA sequencing identifies the expression of hemoglobin in chondrocyte cell subpopulations in osteoarthritis |
| title_full | Single-cell RNA sequencing identifies the expression of hemoglobin in chondrocyte cell subpopulations in osteoarthritis |
| title_fullStr | Single-cell RNA sequencing identifies the expression of hemoglobin in chondrocyte cell subpopulations in osteoarthritis |
| title_full_unstemmed | Single-cell RNA sequencing identifies the expression of hemoglobin in chondrocyte cell subpopulations in osteoarthritis |
| title_short | Single-cell RNA sequencing identifies the expression of hemoglobin in chondrocyte cell subpopulations in osteoarthritis |
| title_sort | single cell rna sequencing identifies the expression of hemoglobin in chondrocyte cell subpopulations in osteoarthritis |
| topic | Single-cell sequencing Hemoglobin Osteoarthritis Chondrocyte |
| url | https://doi.org/10.1186/s12860-024-00519-3 |
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