Combined metabolomic and transcriptomic analysis reveals the key genes for triterpenoid biosynthesis in Cyclocarya paliurus
Abstract Background Cyclocarya paliurus is a high-value tree, and it contains a variety of bioactive secondary metabolites which have broad application prospects in medicine, food and health care. Triterpenoids can improve the bioactive function of C. paliurus health tea and also improve the efficac...
Saved in:
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
BMC
2024-12-01
|
| Series: | BMC Genomics |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s12864-024-11125-0 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1846112816663101440 |
|---|---|
| author | Duo Chen Xupeng Chen Xuehai Zheng Jinmao Zhu Ting Xue |
| author_facet | Duo Chen Xupeng Chen Xuehai Zheng Jinmao Zhu Ting Xue |
| author_sort | Duo Chen |
| collection | DOAJ |
| description | Abstract Background Cyclocarya paliurus is a high-value tree, and it contains a variety of bioactive secondary metabolites which have broad application prospects in medicine, food and health care. Triterpenoids can improve the bioactive function of C. paliurus health tea and also improve the efficacy of health care tea. Results The results of this study showed that there were 69 kinds were terpenoids, and triterpenoids accounted for more than 80%. We excavated 5 kinds of triterpenoid metabolites with high content and significant difference dynamics, namely, corosolic acid, asiatic acid, maslinic acid, ursolic acid and oleanolic acid. The co-expression analysis identified CYP71D8 and CYP716A15 co-expressed with β-AS may generate oleanane type triterpenoids by modifying β-amyrin, while CYP71AN24 and CYP98A2 co-expressed with LUS may play a key role in lupine type triterpenoids biosynthesis. MYB,Whirly,WRKY and bHLH families, which showed strong correlation with function genes, may play an important role in the regulation of P450 and OSC expression. A total of 20 modules were identified by WGCNA analysis, and CYP71AU50 and CYP716A15 in tan and orange modules may play a major role in the synthesis of oleanolic acid, ursolic acid and asiatic acid, while CYP82D47 in lightcyan 1 module may be the hub gene for the biosynthesis of corosolic acid and maslinic acid. Conclusions Our findings mined candidate genes closely related to triterpenoid synthesis in C. paliurus. The results of this paper can provide scientific reference for breeding high-content triterpenoid varieties of C. paliurus. |
| format | Article |
| id | doaj-art-f519b97152434b69b027aa8a24a1d7d3 |
| institution | Kabale University |
| issn | 1471-2164 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Genomics |
| spelling | doaj-art-f519b97152434b69b027aa8a24a1d7d32024-12-22T12:16:50ZengBMCBMC Genomics1471-21642024-12-0125111910.1186/s12864-024-11125-0Combined metabolomic and transcriptomic analysis reveals the key genes for triterpenoid biosynthesis in Cyclocarya paliurusDuo Chen0Xupeng Chen1Xuehai Zheng2Jinmao Zhu3Ting Xue4The Public Service Platform for Industrialization Development Technology of Marine Biological Medicine and Products of the State Oceanic Administration, Fujian Key Laboratory of Special Marine Bioresource Sustainable Utilization, Southern Institute of Oceanography, Key Laboratory of Developmental and Neural Biology College of Life Sciences, Fujian Normal UniversityThe Public Service Platform for Industrialization Development Technology of Marine Biological Medicine and Products of the State Oceanic Administration, Fujian Key Laboratory of Special Marine Bioresource Sustainable Utilization, Southern Institute of Oceanography, Key Laboratory of Developmental and Neural Biology College of Life Sciences, Fujian Normal UniversityThe Public Service Platform for Industrialization Development Technology of Marine Biological Medicine and Products of the State Oceanic Administration, Fujian Key Laboratory of Special Marine Bioresource Sustainable Utilization, Southern Institute of Oceanography, Key Laboratory of Developmental and Neural Biology College of Life Sciences, Fujian Normal UniversityThe Public Service Platform for Industrialization Development Technology of Marine Biological Medicine and Products of the State Oceanic Administration, Fujian Key Laboratory of Special Marine Bioresource Sustainable Utilization, Southern Institute of Oceanography, Key Laboratory of Developmental and Neural Biology College of Life Sciences, Fujian Normal UniversityThe Public Service Platform for Industrialization Development Technology of Marine Biological Medicine and Products of the State Oceanic Administration, Fujian Key Laboratory of Special Marine Bioresource Sustainable Utilization, Southern Institute of Oceanography, Key Laboratory of Developmental and Neural Biology College of Life Sciences, Fujian Normal UniversityAbstract Background Cyclocarya paliurus is a high-value tree, and it contains a variety of bioactive secondary metabolites which have broad application prospects in medicine, food and health care. Triterpenoids can improve the bioactive function of C. paliurus health tea and also improve the efficacy of health care tea. Results The results of this study showed that there were 69 kinds were terpenoids, and triterpenoids accounted for more than 80%. We excavated 5 kinds of triterpenoid metabolites with high content and significant difference dynamics, namely, corosolic acid, asiatic acid, maslinic acid, ursolic acid and oleanolic acid. The co-expression analysis identified CYP71D8 and CYP716A15 co-expressed with β-AS may generate oleanane type triterpenoids by modifying β-amyrin, while CYP71AN24 and CYP98A2 co-expressed with LUS may play a key role in lupine type triterpenoids biosynthesis. MYB,Whirly,WRKY and bHLH families, which showed strong correlation with function genes, may play an important role in the regulation of P450 and OSC expression. A total of 20 modules were identified by WGCNA analysis, and CYP71AU50 and CYP716A15 in tan and orange modules may play a major role in the synthesis of oleanolic acid, ursolic acid and asiatic acid, while CYP82D47 in lightcyan 1 module may be the hub gene for the biosynthesis of corosolic acid and maslinic acid. Conclusions Our findings mined candidate genes closely related to triterpenoid synthesis in C. paliurus. The results of this paper can provide scientific reference for breeding high-content triterpenoid varieties of C. paliurus.https://doi.org/10.1186/s12864-024-11125-0Cyclocarya paliurusTriterpenoid biosynthesisMetabolomicsTranscriptomicsWGCNA |
| spellingShingle | Duo Chen Xupeng Chen Xuehai Zheng Jinmao Zhu Ting Xue Combined metabolomic and transcriptomic analysis reveals the key genes for triterpenoid biosynthesis in Cyclocarya paliurus BMC Genomics Cyclocarya paliurus Triterpenoid biosynthesis Metabolomics Transcriptomics WGCNA |
| title | Combined metabolomic and transcriptomic analysis reveals the key genes for triterpenoid biosynthesis in Cyclocarya paliurus |
| title_full | Combined metabolomic and transcriptomic analysis reveals the key genes for triterpenoid biosynthesis in Cyclocarya paliurus |
| title_fullStr | Combined metabolomic and transcriptomic analysis reveals the key genes for triterpenoid biosynthesis in Cyclocarya paliurus |
| title_full_unstemmed | Combined metabolomic and transcriptomic analysis reveals the key genes for triterpenoid biosynthesis in Cyclocarya paliurus |
| title_short | Combined metabolomic and transcriptomic analysis reveals the key genes for triterpenoid biosynthesis in Cyclocarya paliurus |
| title_sort | combined metabolomic and transcriptomic analysis reveals the key genes for triterpenoid biosynthesis in cyclocarya paliurus |
| topic | Cyclocarya paliurus Triterpenoid biosynthesis Metabolomics Transcriptomics WGCNA |
| url | https://doi.org/10.1186/s12864-024-11125-0 |
| work_keys_str_mv | AT duochen combinedmetabolomicandtranscriptomicanalysisrevealsthekeygenesfortriterpenoidbiosynthesisincyclocaryapaliurus AT xupengchen combinedmetabolomicandtranscriptomicanalysisrevealsthekeygenesfortriterpenoidbiosynthesisincyclocaryapaliurus AT xuehaizheng combinedmetabolomicandtranscriptomicanalysisrevealsthekeygenesfortriterpenoidbiosynthesisincyclocaryapaliurus AT jinmaozhu combinedmetabolomicandtranscriptomicanalysisrevealsthekeygenesfortriterpenoidbiosynthesisincyclocaryapaliurus AT tingxue combinedmetabolomicandtranscriptomicanalysisrevealsthekeygenesfortriterpenoidbiosynthesisincyclocaryapaliurus |