Endoplasmic reticulum stress improved chicken tenderness, promoted apoptosis and autophagy during postmortem ageing
In this study, endoplasmic reticulum (ER) stress inducer tunicamycin (TM) and inhibitor 4-phenylbutyric acid (4-PBA) were used to treat postmortem chicken breast muscle to investigate changes in tenderness and effects on apoptosis and autophagy during 5 days ageing. TM-induced ER stress reduced shea...
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| Format: | Article |
| Language: | English |
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Tsinghua University Press
2024-07-01
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| Series: | Food Science and Human Wellness |
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| Online Access: | https://www.sciopen.com/article/10.26599/FSHW.2022.9250178 |
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| author | Yuwei Chai Lin Chen Shuya Xiang Linxuan Wu Xuebo Liu Jun Luo Xianchao Feng |
| author_facet | Yuwei Chai Lin Chen Shuya Xiang Linxuan Wu Xuebo Liu Jun Luo Xianchao Feng |
| author_sort | Yuwei Chai |
| collection | DOAJ |
| description | In this study, endoplasmic reticulum (ER) stress inducer tunicamycin (TM) and inhibitor 4-phenylbutyric acid (4-PBA) were used to treat postmortem chicken breast muscle to investigate changes in tenderness and effects on apoptosis and autophagy during 5 days ageing. TM-induced ER stress reduced shear force, enhanced myofibril fragmentation index (MFI), disrupted myofibril structure, increased desmin degradation, and activated μ-calpain and caspase-12. In addition, TM-induced ER stress increased the expression of Bax, Bim, and cytochrome c, and decreased the expression of Bcl-xL. Furthermore, TM-induced ER stress improved the conversion of LC3I to LC3II, raised the expression of Beclin-1, and decreased the expression of p62, PI3K, and mTOR. The opposite results were observed after 4-PBA treatment. These results suggested that ER stress could improve chicken tenderness, promote apoptosis and autophagy during chicken postmortem ageing. |
| format | Article |
| id | doaj-art-f4dfc68ef6414f30b5ccbef90e543565 |
| institution | Kabale University |
| issn | 2097-0765 2213-4530 |
| language | English |
| publishDate | 2024-07-01 |
| publisher | Tsinghua University Press |
| record_format | Article |
| series | Food Science and Human Wellness |
| spelling | doaj-art-f4dfc68ef6414f30b5ccbef90e5435652025-01-10T06:56:48ZengTsinghua University PressFood Science and Human Wellness2097-07652213-45302024-07-011342141215110.26599/FSHW.2022.9250178Endoplasmic reticulum stress improved chicken tenderness, promoted apoptosis and autophagy during postmortem ageingYuwei Chai0Lin Chen1Shuya Xiang2Linxuan Wu3Xuebo Liu4Jun Luo5Xianchao Feng6College of Food Science and Engineering, Northwest A&F University, Yangling 712100, ChinaCollege of Food Science and Engineering, Northwest A&F University, Yangling 712100, ChinaCollege of Food Science and Engineering, Northwest A&F University, Yangling 712100, ChinaCollege of Food Science and Engineering, Northwest A&F University, Yangling 712100, ChinaCollege of Food Science and Engineering, Northwest A&F University, Yangling 712100, ChinaCollege of Animal Science and Technology, Northwest A&F University, Yangling 712100, ChinaCollege of Food Science and Engineering, Northwest A&F University, Yangling 712100, ChinaIn this study, endoplasmic reticulum (ER) stress inducer tunicamycin (TM) and inhibitor 4-phenylbutyric acid (4-PBA) were used to treat postmortem chicken breast muscle to investigate changes in tenderness and effects on apoptosis and autophagy during 5 days ageing. TM-induced ER stress reduced shear force, enhanced myofibril fragmentation index (MFI), disrupted myofibril structure, increased desmin degradation, and activated μ-calpain and caspase-12. In addition, TM-induced ER stress increased the expression of Bax, Bim, and cytochrome c, and decreased the expression of Bcl-xL. Furthermore, TM-induced ER stress improved the conversion of LC3I to LC3II, raised the expression of Beclin-1, and decreased the expression of p62, PI3K, and mTOR. The opposite results were observed after 4-PBA treatment. These results suggested that ER stress could improve chicken tenderness, promote apoptosis and autophagy during chicken postmortem ageing.https://www.sciopen.com/article/10.26599/FSHW.2022.9250178endoplasmic reticulum stresstendernessapoptosisautophagychicken |
| spellingShingle | Yuwei Chai Lin Chen Shuya Xiang Linxuan Wu Xuebo Liu Jun Luo Xianchao Feng Endoplasmic reticulum stress improved chicken tenderness, promoted apoptosis and autophagy during postmortem ageing Food Science and Human Wellness endoplasmic reticulum stress tenderness apoptosis autophagy chicken |
| title | Endoplasmic reticulum stress improved chicken tenderness, promoted apoptosis and autophagy during postmortem ageing |
| title_full | Endoplasmic reticulum stress improved chicken tenderness, promoted apoptosis and autophagy during postmortem ageing |
| title_fullStr | Endoplasmic reticulum stress improved chicken tenderness, promoted apoptosis and autophagy during postmortem ageing |
| title_full_unstemmed | Endoplasmic reticulum stress improved chicken tenderness, promoted apoptosis and autophagy during postmortem ageing |
| title_short | Endoplasmic reticulum stress improved chicken tenderness, promoted apoptosis and autophagy during postmortem ageing |
| title_sort | endoplasmic reticulum stress improved chicken tenderness promoted apoptosis and autophagy during postmortem ageing |
| topic | endoplasmic reticulum stress tenderness apoptosis autophagy chicken |
| url | https://www.sciopen.com/article/10.26599/FSHW.2022.9250178 |
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