Genome assembly of a nocturnal butterfly (Macrosoma leucophasiata) reveals convergent adaptation of visual genes

Abstract Nearly all animals exhibit a preferred period of daily activity (diel-niche), strongly influenced by the light environment. Vision is a sensory system that is strongly adapted to light, and evolutionary transitions to novel light environments can impose strong constraints on eye evolution,...

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Main Authors: Rachit Pratap Singh, Yi-Ming Weng, Yash Sondhi, David Plotkin, Paul B. Frandsen, Akito Y. Kawahara
Format: Article
Language:English
Published: Nature Portfolio 2024-12-01
Series:Communications Biology
Online Access:https://doi.org/10.1038/s42003-024-07124-2
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author Rachit Pratap Singh
Yi-Ming Weng
Yash Sondhi
David Plotkin
Paul B. Frandsen
Akito Y. Kawahara
author_facet Rachit Pratap Singh
Yi-Ming Weng
Yash Sondhi
David Plotkin
Paul B. Frandsen
Akito Y. Kawahara
author_sort Rachit Pratap Singh
collection DOAJ
description Abstract Nearly all animals exhibit a preferred period of daily activity (diel-niche), strongly influenced by the light environment. Vision is a sensory system that is strongly adapted to light, and evolutionary transitions to novel light environments can impose strong constraints on eye evolution, color, and motion vision. While the genetic and neural basis of visual adaptation are well-studied in a few model systems, our understanding across the tree of life remains incomplete. Butterflies and moths are an ideal system to investigate the association between gene evolution and diel-niche transitions. While most butterflies are day-flying, hedylid butterflies are unique in being primarily nocturnal, representing an important evolutionary shift from diurnality to nocturnality. We sequenced the first Hedylidae genome and annotated it to understand genomic changes associated with diel niche shifts. Comparing Hedylidae visual genes to those of other diurnal and nocturnal Lepidoptera revealed that visual genes are highly conserved, with no major losses. However, hedylid opsins were more similar to nocturnal moths than their diurnal congeners, suggesting that these opsins convergently evovled to adapt to the nocturnal environment. Evolutionary rate tests (dN/dS) confirmed strong selection on color vision opsins, with some sites being mapped to the functional domain of the blue opsin. Our study provides new insight into the molecular evolutionary adaptations associated with species’ changes to new light environments.
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spelling doaj-art-f4557f239e8d4a62be75d3d617dfa90d2024-12-22T12:42:21ZengNature PortfolioCommunications Biology2399-36422024-12-017111210.1038/s42003-024-07124-2Genome assembly of a nocturnal butterfly (Macrosoma leucophasiata) reveals convergent adaptation of visual genesRachit Pratap Singh0Yi-Ming Weng1Yash Sondhi2David Plotkin3Paul B. Frandsen4Akito Y. Kawahara5McGuire Center for Lepidoptera and Biodiversity, Florida Museum of Natural History, University of FloridaMcGuire Center for Lepidoptera and Biodiversity, Florida Museum of Natural History, University of FloridaMcGuire Center for Lepidoptera and Biodiversity, Florida Museum of Natural History, University of FloridaMcGuire Center for Lepidoptera and Biodiversity, Florida Museum of Natural History, University of FloridaDepartment of Plant and Wildlife Sciences, Brigham Young UniversityMcGuire Center for Lepidoptera and Biodiversity, Florida Museum of Natural History, University of FloridaAbstract Nearly all animals exhibit a preferred period of daily activity (diel-niche), strongly influenced by the light environment. Vision is a sensory system that is strongly adapted to light, and evolutionary transitions to novel light environments can impose strong constraints on eye evolution, color, and motion vision. While the genetic and neural basis of visual adaptation are well-studied in a few model systems, our understanding across the tree of life remains incomplete. Butterflies and moths are an ideal system to investigate the association between gene evolution and diel-niche transitions. While most butterflies are day-flying, hedylid butterflies are unique in being primarily nocturnal, representing an important evolutionary shift from diurnality to nocturnality. We sequenced the first Hedylidae genome and annotated it to understand genomic changes associated with diel niche shifts. Comparing Hedylidae visual genes to those of other diurnal and nocturnal Lepidoptera revealed that visual genes are highly conserved, with no major losses. However, hedylid opsins were more similar to nocturnal moths than their diurnal congeners, suggesting that these opsins convergently evovled to adapt to the nocturnal environment. Evolutionary rate tests (dN/dS) confirmed strong selection on color vision opsins, with some sites being mapped to the functional domain of the blue opsin. Our study provides new insight into the molecular evolutionary adaptations associated with species’ changes to new light environments.https://doi.org/10.1038/s42003-024-07124-2
spellingShingle Rachit Pratap Singh
Yi-Ming Weng
Yash Sondhi
David Plotkin
Paul B. Frandsen
Akito Y. Kawahara
Genome assembly of a nocturnal butterfly (Macrosoma leucophasiata) reveals convergent adaptation of visual genes
Communications Biology
title Genome assembly of a nocturnal butterfly (Macrosoma leucophasiata) reveals convergent adaptation of visual genes
title_full Genome assembly of a nocturnal butterfly (Macrosoma leucophasiata) reveals convergent adaptation of visual genes
title_fullStr Genome assembly of a nocturnal butterfly (Macrosoma leucophasiata) reveals convergent adaptation of visual genes
title_full_unstemmed Genome assembly of a nocturnal butterfly (Macrosoma leucophasiata) reveals convergent adaptation of visual genes
title_short Genome assembly of a nocturnal butterfly (Macrosoma leucophasiata) reveals convergent adaptation of visual genes
title_sort genome assembly of a nocturnal butterfly macrosoma leucophasiata reveals convergent adaptation of visual genes
url https://doi.org/10.1038/s42003-024-07124-2
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