Gene Expression Analysis for Drought Tolerance in Early Stage of Potato Plant Development
Drought has increasingly affected the yield of <i>Solanum tuberosum</i> L. (potato) every year over the last decade, posing serious economic problems for the global agricultural industry. Therefore, it is important to research drought tolerance in plants and obtain more robust varieties...
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2024-10-01
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| author | Rakhim Kanat Malika Shamekova Zagipa Sapakhova Maxat Toishimanov Dias Daurov Nurgul Raissova Zhanar Abilda Ainash Daurova Kabyl Zhambakin |
| author_facet | Rakhim Kanat Malika Shamekova Zagipa Sapakhova Maxat Toishimanov Dias Daurov Nurgul Raissova Zhanar Abilda Ainash Daurova Kabyl Zhambakin |
| author_sort | Rakhim Kanat |
| collection | DOAJ |
| description | Drought has increasingly affected the yield of <i>Solanum tuberosum</i> L. (potato) every year over the last decade, posing serious economic problems for the global agricultural industry. Therefore, it is important to research drought tolerance in plants and obtain more robust varieties of crops. The aim of the present work was to study the expression of drought-upregulated genes in drought-tolerant and drought-sensitive varieties of potato. Bioreactors were used to identify whether each variety was drought-tolerant or drought-sensitive; then, expression analysis was performed according to the morphological characteristics of the plantlets in two different media: Murashige and Skoog (MS) medium and MS medium with 20% PEG-6000 to simulate osmotic stress. Based on the quantitative parameters of six initial varieties, two varieties were selected (Gala and Aksor) for further gene expression analysis. The expression of genes commonly upregulated in drought (<i>ER24</i>, <i>TAS14</i>, <i>DREB147315</i>, <i>PP2C</i>, <i>102605413</i> and <i>NF-YC4</i>) was higher in the drought-tolerant variety than in the sensitive one. Therefore, the expression of these genes can be used to determine the drought tolerance of a potato variety in vitro in the early plant development stage. Moreover, comparative analysis showed that some of the targeted genes used to identify drought tolerance in this study are conserved across different plant species. |
| format | Article |
| id | doaj-art-f40977f9760b48d098e22f2e32bbe8ab |
| institution | Kabale University |
| issn | 2079-7737 |
| language | English |
| publishDate | 2024-10-01 |
| publisher | MDPI AG |
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| series | Biology |
| spelling | doaj-art-f40977f9760b48d098e22f2e32bbe8ab2024-11-26T17:52:14ZengMDPI AGBiology2079-77372024-10-01131185710.3390/biology13110857Gene Expression Analysis for Drought Tolerance in Early Stage of Potato Plant DevelopmentRakhim Kanat0Malika Shamekova1Zagipa Sapakhova2Maxat Toishimanov3Dias Daurov4Nurgul Raissova5Zhanar Abilda6Ainash Daurova7Kabyl Zhambakin8Institute of Plant Biology and Biotechnology, Almaty 050040, KazakhstanInstitute of Plant Biology and Biotechnology, Almaty 050040, KazakhstanInstitute of Plant Biology and Biotechnology, Almaty 050040, KazakhstanInstitute of Plant Biology and Biotechnology, Almaty 050040, KazakhstanInstitute of Plant Biology and Biotechnology, Almaty 050040, KazakhstanInstitute of Plant Biology and Biotechnology, Almaty 050040, KazakhstanInstitute of Plant Biology and Biotechnology, Almaty 050040, KazakhstanInstitute of Plant Biology and Biotechnology, Almaty 050040, KazakhstanInstitute of Plant Biology and Biotechnology, Almaty 050040, KazakhstanDrought has increasingly affected the yield of <i>Solanum tuberosum</i> L. (potato) every year over the last decade, posing serious economic problems for the global agricultural industry. Therefore, it is important to research drought tolerance in plants and obtain more robust varieties of crops. The aim of the present work was to study the expression of drought-upregulated genes in drought-tolerant and drought-sensitive varieties of potato. Bioreactors were used to identify whether each variety was drought-tolerant or drought-sensitive; then, expression analysis was performed according to the morphological characteristics of the plantlets in two different media: Murashige and Skoog (MS) medium and MS medium with 20% PEG-6000 to simulate osmotic stress. Based on the quantitative parameters of six initial varieties, two varieties were selected (Gala and Aksor) for further gene expression analysis. The expression of genes commonly upregulated in drought (<i>ER24</i>, <i>TAS14</i>, <i>DREB147315</i>, <i>PP2C</i>, <i>102605413</i> and <i>NF-YC4</i>) was higher in the drought-tolerant variety than in the sensitive one. Therefore, the expression of these genes can be used to determine the drought tolerance of a potato variety in vitro in the early plant development stage. Moreover, comparative analysis showed that some of the targeted genes used to identify drought tolerance in this study are conserved across different plant species.https://www.mdpi.com/2079-7737/13/11/857<i>Solanum tuberosum</i> L.potatodroughtabiotic stressgene expressionselection |
| spellingShingle | Rakhim Kanat Malika Shamekova Zagipa Sapakhova Maxat Toishimanov Dias Daurov Nurgul Raissova Zhanar Abilda Ainash Daurova Kabyl Zhambakin Gene Expression Analysis for Drought Tolerance in Early Stage of Potato Plant Development Biology <i>Solanum tuberosum</i> L. potato drought abiotic stress gene expression selection |
| title | Gene Expression Analysis for Drought Tolerance in Early Stage of Potato Plant Development |
| title_full | Gene Expression Analysis for Drought Tolerance in Early Stage of Potato Plant Development |
| title_fullStr | Gene Expression Analysis for Drought Tolerance in Early Stage of Potato Plant Development |
| title_full_unstemmed | Gene Expression Analysis for Drought Tolerance in Early Stage of Potato Plant Development |
| title_short | Gene Expression Analysis for Drought Tolerance in Early Stage of Potato Plant Development |
| title_sort | gene expression analysis for drought tolerance in early stage of potato plant development |
| topic | <i>Solanum tuberosum</i> L. potato drought abiotic stress gene expression selection |
| url | https://www.mdpi.com/2079-7737/13/11/857 |
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