De Novo Donor-Specific HLA Antibody Development and Peripheral CD4+CD25high Cells in Kidney Transplant Recipients: A Place for Interaction?
The aim of this study was to determine whether the abundance of regulatory T cells (Tregs) (CD4+CD25high) affects the de novo development of anti-HLA donor-specific antibodies (DSAs) in kidney transplant recipients (KTRs). Methods. Unsensitized (PRA ≤ 10%, no DSA) adult primary KTRs who received a l...
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| Format: | Article |
| Language: | English |
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Wiley
2012-01-01
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| Series: | Journal of Transplantation |
| Online Access: | http://dx.doi.org/10.1155/2012/302539 |
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| author | Josefina Alberu Maria Inés Vargas-Rojas Luis E. Morales-Buenrostro Jose C. Crispin Roxana Rodríguez-Romo Norma O. Uribe-Uribe Gabriel Carrasco Diana Gómez-Martín Jorge Alcocer-Varela |
| author_facet | Josefina Alberu Maria Inés Vargas-Rojas Luis E. Morales-Buenrostro Jose C. Crispin Roxana Rodríguez-Romo Norma O. Uribe-Uribe Gabriel Carrasco Diana Gómez-Martín Jorge Alcocer-Varela |
| author_sort | Josefina Alberu |
| collection | DOAJ |
| description | The aim of this study was to determine whether the abundance of regulatory T cells (Tregs) (CD4+CD25high) affects the de novo development of anti-HLA donor-specific antibodies (DSAs) in kidney transplant recipients (KTRs). Methods. Unsensitized (PRA ≤ 10%, no DSA) adult primary KTRs who received a living (83%) or deceased (17%) KT in our Institution during 2004/2005 were included. DSA testing was performed monthly, and Tregs were quantified by flow cytometry every 3 months, during the 1st year after KT. All patients received triple drug immunosuppressive therapy (CNI + MMF or AZA + PDN); 83% received anti-CD25. Results. 53 KTRs were included; 32% developed DSA during the 1st year after KT. Significantly lower 7-year graft survival was observed in those who developed DSA. No difference was observed in Treg numbers up to 9 months after KT, between DSA positive and negative. However, at 12 months after KT, DSA-negative patients had significantly higher numbers of Treg. Conclusions. Early development of DSA was not associated to variations in Treg abundance. The differences in Treg numbers observed at the late time point may reflect better immune acceptance of the graft and may be associated to long-term effects. Additional inhibitory mechanisms participating earlier in DSA development after KT deserve to be sought. |
| format | Article |
| id | doaj-art-f3fbc36b8dfc45e7a8411881cbe1c00c |
| institution | Kabale University |
| issn | 2090-0007 2090-0015 |
| language | English |
| publishDate | 2012-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Transplantation |
| spelling | doaj-art-f3fbc36b8dfc45e7a8411881cbe1c00c2025-02-03T05:47:31ZengWileyJournal of Transplantation2090-00072090-00152012-01-01201210.1155/2012/302539302539De Novo Donor-Specific HLA Antibody Development and Peripheral CD4+CD25high Cells in Kidney Transplant Recipients: A Place for Interaction?Josefina Alberu0Maria Inés Vargas-Rojas1Luis E. Morales-Buenrostro2Jose C. Crispin3Roxana Rodríguez-Romo4Norma O. Uribe-Uribe5Gabriel Carrasco6Diana Gómez-Martín7Jorge Alcocer-Varela8Department of Transplantation, National Institute of Medical Science and Nutrition Salvador Zubirán, Vasco de Quiroga 15, Colonia Sección XVI, DF, 14000 Mexico City, MexicoDepartment of Immunology, National Institute of Medical Science and Nutrition Salvador Zubirán, Vasco de Quiroga 15, Colonia Sección XVI, DF, 14000 Mexico City, MexicoDepartment of Transplantation, National Institute of Medical Science and Nutrition Salvador Zubirán, Vasco de Quiroga 15, Colonia Sección XVI, DF, 14000 Mexico City, MexicoDepartment of Immunology, National Institute of Medical Science and Nutrition Salvador Zubirán, Vasco de Quiroga 15, Colonia Sección XVI, DF, 14000 Mexico City, MexicoDepartment of Transplantation, National Institute of Medical Science and Nutrition Salvador Zubirán, Vasco de Quiroga 15, Colonia Sección XVI, DF, 14000 Mexico City, MexicoDepartment of Pathology, National Institute of Medical Science and Nutrition Salvador Zubirán, Vasco de Quiroga 15, Colonia Sección XVI, DF, 14000 Mexico City, MexicoDepartment of Immunology, National Institute of Medical Science and Nutrition Salvador Zubirán, Vasco de Quiroga 15, Colonia Sección XVI, DF, 14000 Mexico City, MexicoDepartment of Immunology, National Institute of Medical Science and Nutrition Salvador Zubirán, Vasco de Quiroga 15, Colonia Sección XVI, DF, 14000 Mexico City, MexicoDepartment of Immunology, National Institute of Medical Science and Nutrition Salvador Zubirán, Vasco de Quiroga 15, Colonia Sección XVI, DF, 14000 Mexico City, MexicoThe aim of this study was to determine whether the abundance of regulatory T cells (Tregs) (CD4+CD25high) affects the de novo development of anti-HLA donor-specific antibodies (DSAs) in kidney transplant recipients (KTRs). Methods. Unsensitized (PRA ≤ 10%, no DSA) adult primary KTRs who received a living (83%) or deceased (17%) KT in our Institution during 2004/2005 were included. DSA testing was performed monthly, and Tregs were quantified by flow cytometry every 3 months, during the 1st year after KT. All patients received triple drug immunosuppressive therapy (CNI + MMF or AZA + PDN); 83% received anti-CD25. Results. 53 KTRs were included; 32% developed DSA during the 1st year after KT. Significantly lower 7-year graft survival was observed in those who developed DSA. No difference was observed in Treg numbers up to 9 months after KT, between DSA positive and negative. However, at 12 months after KT, DSA-negative patients had significantly higher numbers of Treg. Conclusions. Early development of DSA was not associated to variations in Treg abundance. The differences in Treg numbers observed at the late time point may reflect better immune acceptance of the graft and may be associated to long-term effects. Additional inhibitory mechanisms participating earlier in DSA development after KT deserve to be sought.http://dx.doi.org/10.1155/2012/302539 |
| spellingShingle | Josefina Alberu Maria Inés Vargas-Rojas Luis E. Morales-Buenrostro Jose C. Crispin Roxana Rodríguez-Romo Norma O. Uribe-Uribe Gabriel Carrasco Diana Gómez-Martín Jorge Alcocer-Varela De Novo Donor-Specific HLA Antibody Development and Peripheral CD4+CD25high Cells in Kidney Transplant Recipients: A Place for Interaction? Journal of Transplantation |
| title | De Novo Donor-Specific HLA Antibody Development and Peripheral CD4+CD25high Cells in Kidney Transplant Recipients: A Place for Interaction? |
| title_full | De Novo Donor-Specific HLA Antibody Development and Peripheral CD4+CD25high Cells in Kidney Transplant Recipients: A Place for Interaction? |
| title_fullStr | De Novo Donor-Specific HLA Antibody Development and Peripheral CD4+CD25high Cells in Kidney Transplant Recipients: A Place for Interaction? |
| title_full_unstemmed | De Novo Donor-Specific HLA Antibody Development and Peripheral CD4+CD25high Cells in Kidney Transplant Recipients: A Place for Interaction? |
| title_short | De Novo Donor-Specific HLA Antibody Development and Peripheral CD4+CD25high Cells in Kidney Transplant Recipients: A Place for Interaction? |
| title_sort | de novo donor specific hla antibody development and peripheral cd4 cd25high cells in kidney transplant recipients a place for interaction |
| url | http://dx.doi.org/10.1155/2012/302539 |
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