A replication-incompetent adenoviral vector encoding for HSV-2 gD2 is immunogenic and protective against HSV-2 intravaginal challenge in mice.
Herpes Simplex virus (HSV) is the cause of genital herpes and no prophylactic treatment is currently available. Replication-incompetent adenoviral vectors are potent inducers of humoral and cellular immune responses in humans. We have designed an adenoviral vector type 35 (Ad35)-based vaccine encodi...
Saved in:
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2024-01-01
|
| Series: | PLoS ONE |
| Online Access: | https://doi.org/10.1371/journal.pone.0310250 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1841555645140041728 |
|---|---|
| author | Elisa Rossetti Marija Vujadinovic Ella van Huizen Jeroen Tolboom Hanneke Schuitemaker Feng Yao Roland Zahn Eirikur Saeland |
| author_facet | Elisa Rossetti Marija Vujadinovic Ella van Huizen Jeroen Tolboom Hanneke Schuitemaker Feng Yao Roland Zahn Eirikur Saeland |
| author_sort | Elisa Rossetti |
| collection | DOAJ |
| description | Herpes Simplex virus (HSV) is the cause of genital herpes and no prophylactic treatment is currently available. Replication-incompetent adenoviral vectors are potent inducers of humoral and cellular immune responses in humans. We have designed an adenoviral vector type 35 (Ad35)-based vaccine encoding the HSV-2 major surface antigen gD2 (Ad35.HSV.gD2). Immunization of mice with Ad35.HSV.gD2 elicited virus neutralizing antibody titers (VNT) and cellular responses against HSV-2 and HSV-1. While immunity was lower than for CJ2-gD2, both vaccines showed 100% survival against intravaginal challenge with HSV-2 G strain and a strong inverse correlation was observed between HSV-2 infection (as measured by viral shedding) and VNT. A combination of Ad35.HSV.gD2 with Ad35 encoding for gB2 (Ad35.HSV.gB2) resulted in increased VNT and lower infection, compared with Ad35.HSV.gD2 alone. Transfer of immune serum into naïve BALB/c mice before intravaginal challenge confirmed the role of antibodies in the protection of mice against infection although other immune factors may play a role as well. |
| format | Article |
| id | doaj-art-f37dd7c3fbe1467aac4e93116c8651e3 |
| institution | Kabale University |
| issn | 1932-6203 |
| language | English |
| publishDate | 2024-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj-art-f37dd7c3fbe1467aac4e93116c8651e32025-01-08T05:32:01ZengPublic Library of Science (PLoS)PLoS ONE1932-62032024-01-011912e031025010.1371/journal.pone.0310250A replication-incompetent adenoviral vector encoding for HSV-2 gD2 is immunogenic and protective against HSV-2 intravaginal challenge in mice.Elisa RossettiMarija VujadinovicElla van HuizenJeroen TolboomHanneke SchuitemakerFeng YaoRoland ZahnEirikur SaelandHerpes Simplex virus (HSV) is the cause of genital herpes and no prophylactic treatment is currently available. Replication-incompetent adenoviral vectors are potent inducers of humoral and cellular immune responses in humans. We have designed an adenoviral vector type 35 (Ad35)-based vaccine encoding the HSV-2 major surface antigen gD2 (Ad35.HSV.gD2). Immunization of mice with Ad35.HSV.gD2 elicited virus neutralizing antibody titers (VNT) and cellular responses against HSV-2 and HSV-1. While immunity was lower than for CJ2-gD2, both vaccines showed 100% survival against intravaginal challenge with HSV-2 G strain and a strong inverse correlation was observed between HSV-2 infection (as measured by viral shedding) and VNT. A combination of Ad35.HSV.gD2 with Ad35 encoding for gB2 (Ad35.HSV.gB2) resulted in increased VNT and lower infection, compared with Ad35.HSV.gD2 alone. Transfer of immune serum into naïve BALB/c mice before intravaginal challenge confirmed the role of antibodies in the protection of mice against infection although other immune factors may play a role as well.https://doi.org/10.1371/journal.pone.0310250 |
| spellingShingle | Elisa Rossetti Marija Vujadinovic Ella van Huizen Jeroen Tolboom Hanneke Schuitemaker Feng Yao Roland Zahn Eirikur Saeland A replication-incompetent adenoviral vector encoding for HSV-2 gD2 is immunogenic and protective against HSV-2 intravaginal challenge in mice. PLoS ONE |
| title | A replication-incompetent adenoviral vector encoding for HSV-2 gD2 is immunogenic and protective against HSV-2 intravaginal challenge in mice. |
| title_full | A replication-incompetent adenoviral vector encoding for HSV-2 gD2 is immunogenic and protective against HSV-2 intravaginal challenge in mice. |
| title_fullStr | A replication-incompetent adenoviral vector encoding for HSV-2 gD2 is immunogenic and protective against HSV-2 intravaginal challenge in mice. |
| title_full_unstemmed | A replication-incompetent adenoviral vector encoding for HSV-2 gD2 is immunogenic and protective against HSV-2 intravaginal challenge in mice. |
| title_short | A replication-incompetent adenoviral vector encoding for HSV-2 gD2 is immunogenic and protective against HSV-2 intravaginal challenge in mice. |
| title_sort | replication incompetent adenoviral vector encoding for hsv 2 gd2 is immunogenic and protective against hsv 2 intravaginal challenge in mice |
| url | https://doi.org/10.1371/journal.pone.0310250 |
| work_keys_str_mv | AT elisarossetti areplicationincompetentadenoviralvectorencodingforhsv2gd2isimmunogenicandprotectiveagainsthsv2intravaginalchallengeinmice AT marijavujadinovic areplicationincompetentadenoviralvectorencodingforhsv2gd2isimmunogenicandprotectiveagainsthsv2intravaginalchallengeinmice AT ellavanhuizen areplicationincompetentadenoviralvectorencodingforhsv2gd2isimmunogenicandprotectiveagainsthsv2intravaginalchallengeinmice AT jeroentolboom areplicationincompetentadenoviralvectorencodingforhsv2gd2isimmunogenicandprotectiveagainsthsv2intravaginalchallengeinmice AT hannekeschuitemaker areplicationincompetentadenoviralvectorencodingforhsv2gd2isimmunogenicandprotectiveagainsthsv2intravaginalchallengeinmice AT fengyao areplicationincompetentadenoviralvectorencodingforhsv2gd2isimmunogenicandprotectiveagainsthsv2intravaginalchallengeinmice AT rolandzahn areplicationincompetentadenoviralvectorencodingforhsv2gd2isimmunogenicandprotectiveagainsthsv2intravaginalchallengeinmice AT eirikursaeland areplicationincompetentadenoviralvectorencodingforhsv2gd2isimmunogenicandprotectiveagainsthsv2intravaginalchallengeinmice AT elisarossetti replicationincompetentadenoviralvectorencodingforhsv2gd2isimmunogenicandprotectiveagainsthsv2intravaginalchallengeinmice AT marijavujadinovic replicationincompetentadenoviralvectorencodingforhsv2gd2isimmunogenicandprotectiveagainsthsv2intravaginalchallengeinmice AT ellavanhuizen replicationincompetentadenoviralvectorencodingforhsv2gd2isimmunogenicandprotectiveagainsthsv2intravaginalchallengeinmice AT jeroentolboom replicationincompetentadenoviralvectorencodingforhsv2gd2isimmunogenicandprotectiveagainsthsv2intravaginalchallengeinmice AT hannekeschuitemaker replicationincompetentadenoviralvectorencodingforhsv2gd2isimmunogenicandprotectiveagainsthsv2intravaginalchallengeinmice AT fengyao replicationincompetentadenoviralvectorencodingforhsv2gd2isimmunogenicandprotectiveagainsthsv2intravaginalchallengeinmice AT rolandzahn replicationincompetentadenoviralvectorencodingforhsv2gd2isimmunogenicandprotectiveagainsthsv2intravaginalchallengeinmice AT eirikursaeland replicationincompetentadenoviralvectorencodingforhsv2gd2isimmunogenicandprotectiveagainsthsv2intravaginalchallengeinmice |