Protocol for inducing beige adipocytes in white adipose tissue of mouse using cold exposure and CL316,243 injection
Summary: White adipose tissue (WAT) beiging holds significant therapeutic potential for combating obesity. Here, we present a protocol for inducing beige WAT in mice using both cold exposure and CL316,243 treatment. We describe steps for intraperitoneal injection, and subcutaneous WAT (sWAT) isolati...
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| Language: | English |
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Elsevier
2025-03-01
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| Series: | STAR Protocols |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2666166724005021 |
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| author | Yalan Wu Chenguang Wang Xiao Yu Tian |
| author_facet | Yalan Wu Chenguang Wang Xiao Yu Tian |
| author_sort | Yalan Wu |
| collection | DOAJ |
| description | Summary: White adipose tissue (WAT) beiging holds significant therapeutic potential for combating obesity. Here, we present a protocol for inducing beige WAT in mice using both cold exposure and CL316,243 treatment. We describe steps for intraperitoneal injection, and subcutaneous WAT (sWAT) isolation, dissection, and fixation. We then detail procedures for histology, whole-mount immunofluorescence (IF) staining, and extracting RNA and protein. This protocol can be used for subsequent analysis to explore the mechanisms governing beige WAT induction in experimental settings, particularly the evaluation of angiogenesis.For complete details on the use and execution of this protocol, please refer to Wang et al.1 : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics. |
| format | Article |
| id | doaj-art-f36f651fa9794eba8476b614adcccf94 |
| institution | Kabale University |
| issn | 2666-1667 |
| language | English |
| publishDate | 2025-03-01 |
| publisher | Elsevier |
| record_format | Article |
| series | STAR Protocols |
| spelling | doaj-art-f36f651fa9794eba8476b614adcccf942025-01-16T04:29:15ZengElsevierSTAR Protocols2666-16672025-03-0161103337Protocol for inducing beige adipocytes in white adipose tissue of mouse using cold exposure and CL316,243 injectionYalan Wu0Chenguang Wang1Xiao Yu Tian2School of Biomedical Sciences, Heart and Vascular Institute, The Chinese University of Hong Kong, Hong Kong SAR, China; Department of Histology and Embryology, School of Basic Medical Sciences, Central South University, Changsha 410013, China; Corresponding authorSchool of Biomedical Sciences, Heart and Vascular Institute, The Chinese University of Hong Kong, Hong Kong SAR, China; Department of Hematology and Oncology, Shenzhen University General Hospital, Shenzhen University, Shenzhen 518071, ChinaSchool of Biomedical Sciences, Heart and Vascular Institute, The Chinese University of Hong Kong, Hong Kong SAR, China; Corresponding authorSummary: White adipose tissue (WAT) beiging holds significant therapeutic potential for combating obesity. Here, we present a protocol for inducing beige WAT in mice using both cold exposure and CL316,243 treatment. We describe steps for intraperitoneal injection, and subcutaneous WAT (sWAT) isolation, dissection, and fixation. We then detail procedures for histology, whole-mount immunofluorescence (IF) staining, and extracting RNA and protein. This protocol can be used for subsequent analysis to explore the mechanisms governing beige WAT induction in experimental settings, particularly the evaluation of angiogenesis.For complete details on the use and execution of this protocol, please refer to Wang et al.1 : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics.http://www.sciencedirect.com/science/article/pii/S2666166724005021Cell BiologyMetabolismModel Organisms |
| spellingShingle | Yalan Wu Chenguang Wang Xiao Yu Tian Protocol for inducing beige adipocytes in white adipose tissue of mouse using cold exposure and CL316,243 injection STAR Protocols Cell Biology Metabolism Model Organisms |
| title | Protocol for inducing beige adipocytes in white adipose tissue of mouse using cold exposure and CL316,243 injection |
| title_full | Protocol for inducing beige adipocytes in white adipose tissue of mouse using cold exposure and CL316,243 injection |
| title_fullStr | Protocol for inducing beige adipocytes in white adipose tissue of mouse using cold exposure and CL316,243 injection |
| title_full_unstemmed | Protocol for inducing beige adipocytes in white adipose tissue of mouse using cold exposure and CL316,243 injection |
| title_short | Protocol for inducing beige adipocytes in white adipose tissue of mouse using cold exposure and CL316,243 injection |
| title_sort | protocol for inducing beige adipocytes in white adipose tissue of mouse using cold exposure and cl316 243 injection |
| topic | Cell Biology Metabolism Model Organisms |
| url | http://www.sciencedirect.com/science/article/pii/S2666166724005021 |
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