Pochonia chlamydosporia synergistically supports systemic plant defense response in Phacelia tanacetifolia against Meloidogyne hapla
The network of antagonistic, neutral, and synergistic interactions between (micro)organisms has moved into the focus of current research, since in agriculture, this knowledge can help to develop efficient biocontrol strategies. Applying the nematophagous fungus Pochonia chlamydosporia as biocontrol...
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Frontiers Media S.A.
2025-01-01
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| Series: | Frontiers in Plant Science |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fpls.2024.1497575/full |
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| author | Jana Könker Jana Könker Sanja Zenker Sanja Zenker Anja Meierhenrich Anja Meierhenrich Anant Patel Karl-Josef Dietz Karl-Josef Dietz |
| author_facet | Jana Könker Jana Könker Sanja Zenker Sanja Zenker Anja Meierhenrich Anja Meierhenrich Anant Patel Karl-Josef Dietz Karl-Josef Dietz |
| author_sort | Jana Könker |
| collection | DOAJ |
| description | The network of antagonistic, neutral, and synergistic interactions between (micro)organisms has moved into the focus of current research, since in agriculture, this knowledge can help to develop efficient biocontrol strategies. Applying the nematophagous fungus Pochonia chlamydosporia as biocontrol agent to manage the root-knot nematode Meloidogyne hapla is a highly promising strategy. To gain new insight into the systemic response of plants to a plant-parasitic nematode and a nematophagous fungus, Phacelia was inoculated with M. hapla and/or P. chlamydosporia and subjected to transcriptome and metabolome analysis of leaves. While the metabolome proved quite stable except for the early time point of 48 h, comparison of the single P. chlamydosporia with the combined treatment revealed even larger effects after 6 d compared to 48 h, aligning with the later root infestation by P. chlamydosporia compared to M. hapla. Simultaneous exposure to both microorganisms showed a stronger overlap with the single M. hapla treatment than P. chlamydosporia. Changes of transcripts and metabolites were higher in the combined treatment compared to the individual inoculations. The results support the conclusion that P. chlamydosporia induces plant defense in a distinct and beneficial manner if combined with M. hapla although plant defense is partly suppressed by the endophytic growth. The results tentatively suggested that the application of P. chlamydosporia as a biocontrol agent against M. hapla can be more effective by supporting these tritrophic interactions with specific additives, such as phytohormones or amino acids in the formulation. |
| format | Article |
| id | doaj-art-f32b26c40a2d4c9ba05385a5d2b45636 |
| institution | Kabale University |
| issn | 1664-462X |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Plant Science |
| spelling | doaj-art-f32b26c40a2d4c9ba05385a5d2b456362025-01-16T06:10:51ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2025-01-011510.3389/fpls.2024.14975751497575Pochonia chlamydosporia synergistically supports systemic plant defense response in Phacelia tanacetifolia against Meloidogyne haplaJana Könker0Jana Könker1Sanja Zenker2Sanja Zenker3Anja Meierhenrich4Anja Meierhenrich5Anant Patel6Karl-Josef Dietz7Karl-Josef Dietz8Plant Biochemistry and Physiology, Bielefeld University, Bielefeld, GermanyFermentation and Formulation of Biologicals and Chemicals, Hochschule Bielefeld - University of Applied Sciences and Arts, Bielefeld, GermanyComputational Biology, Bielefeld University, Bielefeld, GermanyCenter of Biotechnology (CeBiTec), Bielefeld University, Bielefeld, GermanyComputational Biology, Bielefeld University, Bielefeld, GermanyCenter of Biotechnology (CeBiTec), Bielefeld University, Bielefeld, GermanyFermentation and Formulation of Biologicals and Chemicals, Hochschule Bielefeld - University of Applied Sciences and Arts, Bielefeld, GermanyPlant Biochemistry and Physiology, Bielefeld University, Bielefeld, GermanyCenter of Biotechnology (CeBiTec), Bielefeld University, Bielefeld, GermanyThe network of antagonistic, neutral, and synergistic interactions between (micro)organisms has moved into the focus of current research, since in agriculture, this knowledge can help to develop efficient biocontrol strategies. Applying the nematophagous fungus Pochonia chlamydosporia as biocontrol agent to manage the root-knot nematode Meloidogyne hapla is a highly promising strategy. To gain new insight into the systemic response of plants to a plant-parasitic nematode and a nematophagous fungus, Phacelia was inoculated with M. hapla and/or P. chlamydosporia and subjected to transcriptome and metabolome analysis of leaves. While the metabolome proved quite stable except for the early time point of 48 h, comparison of the single P. chlamydosporia with the combined treatment revealed even larger effects after 6 d compared to 48 h, aligning with the later root infestation by P. chlamydosporia compared to M. hapla. Simultaneous exposure to both microorganisms showed a stronger overlap with the single M. hapla treatment than P. chlamydosporia. Changes of transcripts and metabolites were higher in the combined treatment compared to the individual inoculations. The results support the conclusion that P. chlamydosporia induces plant defense in a distinct and beneficial manner if combined with M. hapla although plant defense is partly suppressed by the endophytic growth. The results tentatively suggested that the application of P. chlamydosporia as a biocontrol agent against M. hapla can be more effective by supporting these tritrophic interactions with specific additives, such as phytohormones or amino acids in the formulation.https://www.frontiersin.org/articles/10.3389/fpls.2024.1497575/fullplant defensebiotic stresssynergistic effectssystemic plant responsebiological controlplant-parasitic nematodes |
| spellingShingle | Jana Könker Jana Könker Sanja Zenker Sanja Zenker Anja Meierhenrich Anja Meierhenrich Anant Patel Karl-Josef Dietz Karl-Josef Dietz Pochonia chlamydosporia synergistically supports systemic plant defense response in Phacelia tanacetifolia against Meloidogyne hapla Frontiers in Plant Science plant defense biotic stress synergistic effects systemic plant response biological control plant-parasitic nematodes |
| title | Pochonia chlamydosporia synergistically supports systemic plant defense response in Phacelia tanacetifolia against Meloidogyne hapla |
| title_full | Pochonia chlamydosporia synergistically supports systemic plant defense response in Phacelia tanacetifolia against Meloidogyne hapla |
| title_fullStr | Pochonia chlamydosporia synergistically supports systemic plant defense response in Phacelia tanacetifolia against Meloidogyne hapla |
| title_full_unstemmed | Pochonia chlamydosporia synergistically supports systemic plant defense response in Phacelia tanacetifolia against Meloidogyne hapla |
| title_short | Pochonia chlamydosporia synergistically supports systemic plant defense response in Phacelia tanacetifolia against Meloidogyne hapla |
| title_sort | pochonia chlamydosporia synergistically supports systemic plant defense response in phacelia tanacetifolia against meloidogyne hapla |
| topic | plant defense biotic stress synergistic effects systemic plant response biological control plant-parasitic nematodes |
| url | https://www.frontiersin.org/articles/10.3389/fpls.2024.1497575/full |
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