Structure characterization of Grifola frondosa polysaccharide and its effect on insulin resistance in HFD-fed mice
Abstract Polysaccharide extracted from Grifola frondosa (GFP) was selected in this study. After preliminary separation, four factions were collected, named GFP-F1, GFP-F2, GFP-F3 and GFP-F4. GPF-F2 was further separated into two fractions, namely GFP-N1 and GFP-N2. The molecular weight of GFP-N1 and...
Saved in:
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Nature Portfolio
2025-01-01
|
| Series: | npj Science of Food |
| Online Access: | https://doi.org/10.1038/s41538-024-00359-7 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1841544329158459392 |
|---|---|
| author | Yin-Yi Ding Jinchi Lan Yuxin Wang Yuxiang Pan Tianyuan Song Shizhu Liu Zhenyu Gu Yujun Ge |
| author_facet | Yin-Yi Ding Jinchi Lan Yuxin Wang Yuxiang Pan Tianyuan Song Shizhu Liu Zhenyu Gu Yujun Ge |
| author_sort | Yin-Yi Ding |
| collection | DOAJ |
| description | Abstract Polysaccharide extracted from Grifola frondosa (GFP) was selected in this study. After preliminary separation, four factions were collected, named GFP-F1, GFP-F2, GFP-F3 and GFP-F4. GPF-F2 was further separated into two fractions, namely GFP-N1 and GFP-N2. The molecular weight of GFP-N1 and GFP-N2 was 3.323×103 kDa and 10.8 kDa, respectively. GFP-N1 was composed of glucose and galactose and 1 → 3, 1 → 4, and 1 → 6 glycosidic bonds. GFP-N2 was composed of glucose, galactose and mannose and 1 → 2, 1 → 3, 1 → 4, and 1 → 6 glycosidic bonds. GFP could significantly relieve the insulin resistance induced by HFD. GFP significantly alleviated gut microbiota disturbance caused by HFD and increased the production of short-chain fatty acids, and further reduced the expression of LPS/TLR4 inflammatory pathway. GFP significantly reduced the oxidative stress induced by HFD, increased the expression of the Nrf2/ARE signaling pathway. These results indicated that GFP could be developed as a potential ingredient for the management of insulin resistance. |
| format | Article |
| id | doaj-art-f326fd1359d848b4af795b34935c47a1 |
| institution | Kabale University |
| issn | 2396-8370 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | npj Science of Food |
| spelling | doaj-art-f326fd1359d848b4af795b34935c47a12025-01-12T12:41:15ZengNature Portfolionpj Science of Food2396-83702025-01-019111510.1038/s41538-024-00359-7Structure characterization of Grifola frondosa polysaccharide and its effect on insulin resistance in HFD-fed miceYin-Yi Ding0Jinchi Lan1Yuxin Wang2Yuxiang Pan3Tianyuan Song4Shizhu Liu5Zhenyu Gu6Yujun Ge7National Experimental Teaching Demonstration Center of Food Engineering and Quality and Safety, Food (Edible Fungi) Processing Technology Research Center, School of Food Science and Biotechnology, Zhejiang Gongshang UniversityNational Experimental Teaching Demonstration Center of Food Engineering and Quality and Safety, Food (Edible Fungi) Processing Technology Research Center, School of Food Science and Biotechnology, Zhejiang Gongshang UniversityNational Experimental Teaching Demonstration Center of Food Engineering and Quality and Safety, Food (Edible Fungi) Processing Technology Research Center, School of Food Science and Biotechnology, Zhejiang Gongshang UniversityNational Experimental Teaching Demonstration Center of Food Engineering and Quality and Safety, Food (Edible Fungi) Processing Technology Research Center, School of Food Science and Biotechnology, Zhejiang Gongshang UniversityNational Experimental Teaching Demonstration Center of Food Engineering and Quality and Safety, Food (Edible Fungi) Processing Technology Research Center, School of Food Science and Biotechnology, Zhejiang Gongshang UniversityZhejiang Fangge Pharmaceutical Co. LtdNational Experimental Teaching Demonstration Center of Food Engineering and Quality and Safety, Food (Edible Fungi) Processing Technology Research Center, School of Food Science and Biotechnology, Zhejiang Gongshang UniversityCentral blood station of JiaxingAbstract Polysaccharide extracted from Grifola frondosa (GFP) was selected in this study. After preliminary separation, four factions were collected, named GFP-F1, GFP-F2, GFP-F3 and GFP-F4. GPF-F2 was further separated into two fractions, namely GFP-N1 and GFP-N2. The molecular weight of GFP-N1 and GFP-N2 was 3.323×103 kDa and 10.8 kDa, respectively. GFP-N1 was composed of glucose and galactose and 1 → 3, 1 → 4, and 1 → 6 glycosidic bonds. GFP-N2 was composed of glucose, galactose and mannose and 1 → 2, 1 → 3, 1 → 4, and 1 → 6 glycosidic bonds. GFP could significantly relieve the insulin resistance induced by HFD. GFP significantly alleviated gut microbiota disturbance caused by HFD and increased the production of short-chain fatty acids, and further reduced the expression of LPS/TLR4 inflammatory pathway. GFP significantly reduced the oxidative stress induced by HFD, increased the expression of the Nrf2/ARE signaling pathway. These results indicated that GFP could be developed as a potential ingredient for the management of insulin resistance.https://doi.org/10.1038/s41538-024-00359-7 |
| spellingShingle | Yin-Yi Ding Jinchi Lan Yuxin Wang Yuxiang Pan Tianyuan Song Shizhu Liu Zhenyu Gu Yujun Ge Structure characterization of Grifola frondosa polysaccharide and its effect on insulin resistance in HFD-fed mice npj Science of Food |
| title | Structure characterization of Grifola frondosa polysaccharide and its effect on insulin resistance in HFD-fed mice |
| title_full | Structure characterization of Grifola frondosa polysaccharide and its effect on insulin resistance in HFD-fed mice |
| title_fullStr | Structure characterization of Grifola frondosa polysaccharide and its effect on insulin resistance in HFD-fed mice |
| title_full_unstemmed | Structure characterization of Grifola frondosa polysaccharide and its effect on insulin resistance in HFD-fed mice |
| title_short | Structure characterization of Grifola frondosa polysaccharide and its effect on insulin resistance in HFD-fed mice |
| title_sort | structure characterization of grifola frondosa polysaccharide and its effect on insulin resistance in hfd fed mice |
| url | https://doi.org/10.1038/s41538-024-00359-7 |
| work_keys_str_mv | AT yinyiding structurecharacterizationofgrifolafrondosapolysaccharideanditseffectoninsulinresistanceinhfdfedmice AT jinchilan structurecharacterizationofgrifolafrondosapolysaccharideanditseffectoninsulinresistanceinhfdfedmice AT yuxinwang structurecharacterizationofgrifolafrondosapolysaccharideanditseffectoninsulinresistanceinhfdfedmice AT yuxiangpan structurecharacterizationofgrifolafrondosapolysaccharideanditseffectoninsulinresistanceinhfdfedmice AT tianyuansong structurecharacterizationofgrifolafrondosapolysaccharideanditseffectoninsulinresistanceinhfdfedmice AT shizhuliu structurecharacterizationofgrifolafrondosapolysaccharideanditseffectoninsulinresistanceinhfdfedmice AT zhenyugu structurecharacterizationofgrifolafrondosapolysaccharideanditseffectoninsulinresistanceinhfdfedmice AT yujunge structurecharacterizationofgrifolafrondosapolysaccharideanditseffectoninsulinresistanceinhfdfedmice |