Bacillus velezensis R22 inhibits the growth of multiple fungal phytopathogens by producing surfactin and four fengycin homologues
Significant agricultural losses are caused by the phytopathogenic fungi Botrytis cinerea and Phytophthora infestans, as well as bacteria of the Ralstonia solanacearum species. The present work aimed to isolate rhizobacteria for simultaneous biocontrol of these three phytopathogenic species and to su...
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2024-12-01
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| Series: | Biotechnology & Biotechnological Equipment |
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| Online Access: | https://www.tandfonline.com/doi/10.1080/13102818.2024.2313072 |
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| author | Nadya Armenova Penka Petrova Maria Gerginova Ekaterina Krumova Dimitar Kaynarov Lyudmila Velkova Pavlina Dolashka Kaloyan Petrov |
| author_facet | Nadya Armenova Penka Petrova Maria Gerginova Ekaterina Krumova Dimitar Kaynarov Lyudmila Velkova Pavlina Dolashka Kaloyan Petrov |
| author_sort | Nadya Armenova |
| collection | DOAJ |
| description | Significant agricultural losses are caused by the phytopathogenic fungi Botrytis cinerea and Phytophthora infestans, as well as bacteria of the Ralstonia solanacearum species. The present work aimed to isolate rhizobacteria for simultaneous biocontrol of these three phytopathogenic species and to suggest the mechanisms of their antagonistic action. Among 120 Bacillus spp. isolated from soils, Bacillus velezensis and Bacillus licheniformis strains displayed the highest activity against all three phytopathogens. A rapid, polymerase chain reaction-based method for detecting nonribosomal peptide synthetase genes was developed to elucidate the genetic basis of these traits. The presence of fenA, srfAA, ppsA, and lchAA genes, encoding fengycin/surfactin/plipastatin synthetases and lichenysin synthase, was revealed in the strains’ genomes. The whole genome sequencing (WGS) of B. velezensis R22 showed that it contains 4,081,504 bp (with G + C content 46.35%), 4087 genes for 3935 proteins, 72 tRNAs, 14 rRNAs, and 5 ncRNAs. WGS allowed the prediction of 10 complete clusters for secondary metabolites with putative antimicrobial activity: difficidin, fengycin, bacillaene, butyrosin, bacillibactin, bacilysin, surfactin, macrolactin H, macrolactin R22, and velezensin. LC-MS and high-sensitivity UHPLC-Q-TOF LC-MS/MS analysis were used to search for the predicted metabolites in cell-free supernatants of B. velezensis R22. The compounds with the strongest antifungal activity are surfactin with a C15 β-OH fatty acid chain; two homologous forms of fengycin A; and two fengycin B homologues containing C16 and C17 β-hydroxy fatty acid chains. The broad antimicrobial spectrum of B. velezensis R22 and its molecular characterization provide a good basis for the future development of plant protection preparations. |
| format | Article |
| id | doaj-art-f2d986224f7347b4b0250ed6029810d9 |
| institution | Kabale University |
| issn | 1310-2818 1314-3530 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | Taylor & Francis Group |
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| series | Biotechnology & Biotechnological Equipment |
| spelling | doaj-art-f2d986224f7347b4b0250ed6029810d92024-12-10T05:42:51ZengTaylor & Francis GroupBiotechnology & Biotechnological Equipment1310-28181314-35302024-12-0138110.1080/13102818.2024.2313072Bacillus velezensis R22 inhibits the growth of multiple fungal phytopathogens by producing surfactin and four fengycin homologuesNadya Armenova0Penka Petrova1Maria Gerginova2Ekaterina Krumova3Dimitar Kaynarov4Lyudmila Velkova5Pavlina Dolashka6Kaloyan Petrov7Department of Biochemical Engineering, Institute of Chemical Engineering, Bulgarian Academy of Sciences, Sofia, BulgariaDepartment of General Microbiology, Institute of Microbiology, Bulgarian Academy of Sciences, Sofia, BulgariaDepartment of General Microbiology, Institute of Microbiology, Bulgarian Academy of Sciences, Sofia, BulgariaDepartment of Mycology, Institute of Microbiology, Bulgarian Academy of Sciences, Sofia, BulgariaDepartment of Chemistry and Biophysics of Proteins and Enzymes, Institute of Organic Chemistry with Centre of Phytochemistry, Bulgarian Academy of Sciences, Sofia, BulgariaDepartment of Chemistry and Biophysics of Proteins and Enzymes, Institute of Organic Chemistry with Centre of Phytochemistry, Bulgarian Academy of Sciences, Sofia, BulgariaDepartment of Chemistry and Biophysics of Proteins and Enzymes, Institute of Organic Chemistry with Centre of Phytochemistry, Bulgarian Academy of Sciences, Sofia, BulgariaDepartment of Biochemical Engineering, Institute of Chemical Engineering, Bulgarian Academy of Sciences, Sofia, BulgariaSignificant agricultural losses are caused by the phytopathogenic fungi Botrytis cinerea and Phytophthora infestans, as well as bacteria of the Ralstonia solanacearum species. The present work aimed to isolate rhizobacteria for simultaneous biocontrol of these three phytopathogenic species and to suggest the mechanisms of their antagonistic action. Among 120 Bacillus spp. isolated from soils, Bacillus velezensis and Bacillus licheniformis strains displayed the highest activity against all three phytopathogens. A rapid, polymerase chain reaction-based method for detecting nonribosomal peptide synthetase genes was developed to elucidate the genetic basis of these traits. The presence of fenA, srfAA, ppsA, and lchAA genes, encoding fengycin/surfactin/plipastatin synthetases and lichenysin synthase, was revealed in the strains’ genomes. The whole genome sequencing (WGS) of B. velezensis R22 showed that it contains 4,081,504 bp (with G + C content 46.35%), 4087 genes for 3935 proteins, 72 tRNAs, 14 rRNAs, and 5 ncRNAs. WGS allowed the prediction of 10 complete clusters for secondary metabolites with putative antimicrobial activity: difficidin, fengycin, bacillaene, butyrosin, bacillibactin, bacilysin, surfactin, macrolactin H, macrolactin R22, and velezensin. LC-MS and high-sensitivity UHPLC-Q-TOF LC-MS/MS analysis were used to search for the predicted metabolites in cell-free supernatants of B. velezensis R22. The compounds with the strongest antifungal activity are surfactin with a C15 β-OH fatty acid chain; two homologous forms of fengycin A; and two fengycin B homologues containing C16 and C17 β-hydroxy fatty acid chains. The broad antimicrobial spectrum of B. velezensis R22 and its molecular characterization provide a good basis for the future development of plant protection preparations.https://www.tandfonline.com/doi/10.1080/13102818.2024.2313072Bacillus velezensisfengycinsurfactinBotrytis cinereaPhytophthora infestansLC-ESI-MS |
| spellingShingle | Nadya Armenova Penka Petrova Maria Gerginova Ekaterina Krumova Dimitar Kaynarov Lyudmila Velkova Pavlina Dolashka Kaloyan Petrov Bacillus velezensis R22 inhibits the growth of multiple fungal phytopathogens by producing surfactin and four fengycin homologues Biotechnology & Biotechnological Equipment Bacillus velezensis fengycin surfactin Botrytis cinerea Phytophthora infestans LC-ESI-MS |
| title | Bacillus velezensis R22 inhibits the growth of multiple fungal phytopathogens by producing surfactin and four fengycin homologues |
| title_full | Bacillus velezensis R22 inhibits the growth of multiple fungal phytopathogens by producing surfactin and four fengycin homologues |
| title_fullStr | Bacillus velezensis R22 inhibits the growth of multiple fungal phytopathogens by producing surfactin and four fengycin homologues |
| title_full_unstemmed | Bacillus velezensis R22 inhibits the growth of multiple fungal phytopathogens by producing surfactin and four fengycin homologues |
| title_short | Bacillus velezensis R22 inhibits the growth of multiple fungal phytopathogens by producing surfactin and four fengycin homologues |
| title_sort | bacillus velezensis r22 inhibits the growth of multiple fungal phytopathogens by producing surfactin and four fengycin homologues |
| topic | Bacillus velezensis fengycin surfactin Botrytis cinerea Phytophthora infestans LC-ESI-MS |
| url | https://www.tandfonline.com/doi/10.1080/13102818.2024.2313072 |
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