Riboflavin- and Dextran-Producing <i>Weissella confusa</i> FS54 B2: Characterization and Testing for Development of Fermented Plant-Based Beverages
The use of lactic acid bacteria for developing functional foods is increasing for their ability to synthesize beneficial metabolites such as vitamin B (riboflavin, RF) and postbiotic compounds. Here, the spontaneous mutant FS54 B2 was isolated by treatment of the dextran-producing <i>Weissella...
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2024-12-01
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| Series: | Foods |
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| author | Malek Lahmar Norhane Besrour-Aouam Annel M. Hernández-Alcántara Iñaki Diez-Ozaeta Imene Fhoula Paloma López Mari Luz Mohedano Hadda-Imene Ouzari |
| author_facet | Malek Lahmar Norhane Besrour-Aouam Annel M. Hernández-Alcántara Iñaki Diez-Ozaeta Imene Fhoula Paloma López Mari Luz Mohedano Hadda-Imene Ouzari |
| author_sort | Malek Lahmar |
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| description | The use of lactic acid bacteria for developing functional foods is increasing for their ability to synthesize beneficial metabolites such as vitamin B (riboflavin, RF) and postbiotic compounds. Here, the spontaneous mutant FS54 B2 was isolated by treatment of the dextran-producing <i>Weissella confusa</i> FS54 strain with roseoflavin. FS54 B2 overproduced RF (4.9 mg/L) in synthetic medium. The FMN riboswitch is responsible for the regulation of RF biosynthesis, and sequencing of the coding DNA revealed that FS54 B2 carries the G131U mutation. FS54 B2 retained the capacity of FS54 to synthesize high levels of dextran (3.8 g/L) in synthetic medium. The fermentation capacities of the two <i>Weissella</i> strains was tested in commercial oat-, soy- and rice-based beverages. The best substrate for FS54 B2 was the oat-based drink, in which, after fermentation, the following were detected: RF (2.4 mg/L), dextran (5.3 mg/L), potential prebiotics (oligosaccharides (panose (5.1 g/L), isomaltose (753 mg/L) and isomaltotriose (454 mg/L)) and the antioxidant mannitol (16.3 g/L). pH-lowering ability and cell viability after one month of storage period were confirmed. As far as we know, this is the first time that an RF-overproducing <i>W. confusa</i> strain has been isolated, characterized and tested for its potential use in the development of functional beverages. |
| format | Article |
| id | doaj-art-f2b64f520c75422e868361848e7b1e88 |
| institution | Kabale University |
| issn | 2304-8158 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | MDPI AG |
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| series | Foods |
| spelling | doaj-art-f2b64f520c75422e868361848e7b1e882024-12-27T14:26:32ZengMDPI AGFoods2304-81582024-12-011324411210.3390/foods13244112Riboflavin- and Dextran-Producing <i>Weissella confusa</i> FS54 B2: Characterization and Testing for Development of Fermented Plant-Based BeveragesMalek Lahmar0Norhane Besrour-Aouam1Annel M. Hernández-Alcántara2Iñaki Diez-Ozaeta3Imene Fhoula4Paloma López5Mari Luz Mohedano6Hadda-Imene Ouzari7Centro de Investigaciones Biológicas Margarita Salas (CIB-CSIC), 28040 Madrid, SpainMicroorganisms and Active Biomolecules Laboratory (LR03ES03), Faculty of Sciences of Tunis, University of Tunis El Manar, Tunis 2092, TunisiaCentro de Investigaciones Biológicas Margarita Salas (CIB-CSIC), 28040 Madrid, SpainCentro de Investigaciones Biológicas Margarita Salas (CIB-CSIC), 28040 Madrid, SpainMicroorganisms and Active Biomolecules Laboratory (LR03ES03), Faculty of Sciences of Tunis, University of Tunis El Manar, Tunis 2092, TunisiaCentro de Investigaciones Biológicas Margarita Salas (CIB-CSIC), 28040 Madrid, SpainCentro de Investigaciones Biológicas Margarita Salas (CIB-CSIC), 28040 Madrid, SpainMicroorganisms and Active Biomolecules Laboratory (LR03ES03), Faculty of Sciences of Tunis, University of Tunis El Manar, Tunis 2092, TunisiaThe use of lactic acid bacteria for developing functional foods is increasing for their ability to synthesize beneficial metabolites such as vitamin B (riboflavin, RF) and postbiotic compounds. Here, the spontaneous mutant FS54 B2 was isolated by treatment of the dextran-producing <i>Weissella confusa</i> FS54 strain with roseoflavin. FS54 B2 overproduced RF (4.9 mg/L) in synthetic medium. The FMN riboswitch is responsible for the regulation of RF biosynthesis, and sequencing of the coding DNA revealed that FS54 B2 carries the G131U mutation. FS54 B2 retained the capacity of FS54 to synthesize high levels of dextran (3.8 g/L) in synthetic medium. The fermentation capacities of the two <i>Weissella</i> strains was tested in commercial oat-, soy- and rice-based beverages. The best substrate for FS54 B2 was the oat-based drink, in which, after fermentation, the following were detected: RF (2.4 mg/L), dextran (5.3 mg/L), potential prebiotics (oligosaccharides (panose (5.1 g/L), isomaltose (753 mg/L) and isomaltotriose (454 mg/L)) and the antioxidant mannitol (16.3 g/L). pH-lowering ability and cell viability after one month of storage period were confirmed. As far as we know, this is the first time that an RF-overproducing <i>W. confusa</i> strain has been isolated, characterized and tested for its potential use in the development of functional beverages.https://www.mdpi.com/2304-8158/13/24/4112lactic acid bacteria<i>Weissella confusa</i>riboflavinFMN riboswitchplant-based beveragesprebiotic oligosaccharides |
| spellingShingle | Malek Lahmar Norhane Besrour-Aouam Annel M. Hernández-Alcántara Iñaki Diez-Ozaeta Imene Fhoula Paloma López Mari Luz Mohedano Hadda-Imene Ouzari Riboflavin- and Dextran-Producing <i>Weissella confusa</i> FS54 B2: Characterization and Testing for Development of Fermented Plant-Based Beverages Foods lactic acid bacteria <i>Weissella confusa</i> riboflavin FMN riboswitch plant-based beverages prebiotic oligosaccharides |
| title | Riboflavin- and Dextran-Producing <i>Weissella confusa</i> FS54 B2: Characterization and Testing for Development of Fermented Plant-Based Beverages |
| title_full | Riboflavin- and Dextran-Producing <i>Weissella confusa</i> FS54 B2: Characterization and Testing for Development of Fermented Plant-Based Beverages |
| title_fullStr | Riboflavin- and Dextran-Producing <i>Weissella confusa</i> FS54 B2: Characterization and Testing for Development of Fermented Plant-Based Beverages |
| title_full_unstemmed | Riboflavin- and Dextran-Producing <i>Weissella confusa</i> FS54 B2: Characterization and Testing for Development of Fermented Plant-Based Beverages |
| title_short | Riboflavin- and Dextran-Producing <i>Weissella confusa</i> FS54 B2: Characterization and Testing for Development of Fermented Plant-Based Beverages |
| title_sort | riboflavin and dextran producing i weissella confusa i fs54 b2 characterization and testing for development of fermented plant based beverages |
| topic | lactic acid bacteria <i>Weissella confusa</i> riboflavin FMN riboswitch plant-based beverages prebiotic oligosaccharides |
| url | https://www.mdpi.com/2304-8158/13/24/4112 |
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