Evaluation of high-value bioproducts production by marine endophytic fungus Arthrinium sp. FAKSA 10 under solid state fermentation using agro-industrial wastes
Background: To address public health challenges, there is increasing interest in bioactive metabolites from unconventional sources for targeted cancer and viral treatments with minimal side effects. Endophytic fungi are promising for these therapies. This study focused on maximizing bioactive agent...
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| Format: | Article |
| Language: | English |
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Elsevier
2025-01-01
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| Series: | Electronic Journal of Biotechnology |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S0717345824000289 |
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| author | Mohammad J. Alsarraf Fuad Ameen Abdullah Alfalih Zirak Sajjad |
| author_facet | Mohammad J. Alsarraf Fuad Ameen Abdullah Alfalih Zirak Sajjad |
| author_sort | Mohammad J. Alsarraf |
| collection | DOAJ |
| description | Background: To address public health challenges, there is increasing interest in bioactive metabolites from unconventional sources for targeted cancer and viral treatments with minimal side effects. Endophytic fungi are promising for these therapies. This study focused on maximizing bioactive agent yields from these fungi using a low-cost medium and optimized fermentation system. Results: Forty-three endophytic fungi isolated from Sinularia polydactyla were cultivated on wheat bran medium and evaluated for bioactive metabolites under solid-state fermentation. Strain FAKSA 10, identified as Arthrinium sp. FAKSA 10, exhibited the highest levels of pharmaceutical metabolites, including L-glutaminase, L-methioninase, L-arginase, L-asparaginase, L-tyrosinase, L-lysine α-oxidase, and ribonuclease, with a 79.12% hepatitis C virus knockdown rate. This strain produced 46 metabolites with anticancer, antioxidant, antiviral, and cytotoxic properties, including major compounds like hexadecanoic acid methyl ester; hexadecanoic acid ethyl ester; 9, 12-octadecadienoic acid (Z, Z), methyl ester; 9-octadecenoic acid (Z)-, methyl ester, and 11, 14-eicosadienoic acid, methyl ester. A cost-effective strategy using 11 agro-industrial residues was applied, followed by optimization of the solid-state fermentation system. This optimization increased enzyme yields and enhanced antiviral and antioxidant activities. The optimal conditions for solid-state fermentation were a 10 d incubation, 1 mm particle size, 60% initial moisture, 28 °C temperature, and 2 × 107 CFU/mL inoculum size. Conclusions: This study exploited Arthrinium sp. FAKSA 10 metabolites as natural pharmaceuticals against cancer and viral diseases, highlighting their significant antioxidant properties. Among various residues, oil cakes emerged as an effective and cost-efficient medium, capable of significantly enhancing the production of valuable bioactive metabolites.How to cite: Alsarraf MJ, Ameen F, Alfalih A, et al. Evaluation of high-value bioproducts production by marine endophytic fungus Arthrinium sp. FAKSA 10 under solid state fermentation using agro-industrial wastes. Electron J Biotechnol 2025;73. https://doi.org/10.1016/j.ejbt.2024.09.001. |
| format | Article |
| id | doaj-art-f28a22024b57462fb735bf082b864608 |
| institution | Kabale University |
| issn | 0717-3458 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Electronic Journal of Biotechnology |
| spelling | doaj-art-f28a22024b57462fb735bf082b8646082025-01-11T06:38:27ZengElsevierElectronic Journal of Biotechnology0717-34582025-01-0173117Evaluation of high-value bioproducts production by marine endophytic fungus Arthrinium sp. FAKSA 10 under solid state fermentation using agro-industrial wastesMohammad J. Alsarraf0Fuad Ameen1Abdullah Alfalih2Zirak Sajjad3Department of Science, College of Basic Education, The Public Authority of Applied Education and Training, Safat, KuwaitDepartment of Botany & Microbiology, College of Science, King Saud University, Riyadh, Saudi Arabia; Corresponding author.Department of Botany & Microbiology, College of Science, King Saud University, Riyadh, Saudi ArabiaCollege of Human Medicine, Michigan State University, East Lansing, USABackground: To address public health challenges, there is increasing interest in bioactive metabolites from unconventional sources for targeted cancer and viral treatments with minimal side effects. Endophytic fungi are promising for these therapies. This study focused on maximizing bioactive agent yields from these fungi using a low-cost medium and optimized fermentation system. Results: Forty-three endophytic fungi isolated from Sinularia polydactyla were cultivated on wheat bran medium and evaluated for bioactive metabolites under solid-state fermentation. Strain FAKSA 10, identified as Arthrinium sp. FAKSA 10, exhibited the highest levels of pharmaceutical metabolites, including L-glutaminase, L-methioninase, L-arginase, L-asparaginase, L-tyrosinase, L-lysine α-oxidase, and ribonuclease, with a 79.12% hepatitis C virus knockdown rate. This strain produced 46 metabolites with anticancer, antioxidant, antiviral, and cytotoxic properties, including major compounds like hexadecanoic acid methyl ester; hexadecanoic acid ethyl ester; 9, 12-octadecadienoic acid (Z, Z), methyl ester; 9-octadecenoic acid (Z)-, methyl ester, and 11, 14-eicosadienoic acid, methyl ester. A cost-effective strategy using 11 agro-industrial residues was applied, followed by optimization of the solid-state fermentation system. This optimization increased enzyme yields and enhanced antiviral and antioxidant activities. The optimal conditions for solid-state fermentation were a 10 d incubation, 1 mm particle size, 60% initial moisture, 28 °C temperature, and 2 × 107 CFU/mL inoculum size. Conclusions: This study exploited Arthrinium sp. FAKSA 10 metabolites as natural pharmaceuticals against cancer and viral diseases, highlighting their significant antioxidant properties. Among various residues, oil cakes emerged as an effective and cost-efficient medium, capable of significantly enhancing the production of valuable bioactive metabolites.How to cite: Alsarraf MJ, Ameen F, Alfalih A, et al. Evaluation of high-value bioproducts production by marine endophytic fungus Arthrinium sp. FAKSA 10 under solid state fermentation using agro-industrial wastes. Electron J Biotechnol 2025;73. https://doi.org/10.1016/j.ejbt.2024.09.001.http://www.sciencedirect.com/science/article/pii/S0717345824000289Agro-industrial wastesAnticancer enzymesAnti-HCVAntimicrobialAntioxidantArthrinium sp. |
| spellingShingle | Mohammad J. Alsarraf Fuad Ameen Abdullah Alfalih Zirak Sajjad Evaluation of high-value bioproducts production by marine endophytic fungus Arthrinium sp. FAKSA 10 under solid state fermentation using agro-industrial wastes Electronic Journal of Biotechnology Agro-industrial wastes Anticancer enzymes Anti-HCV Antimicrobial Antioxidant Arthrinium sp. |
| title | Evaluation of high-value bioproducts production by marine endophytic fungus Arthrinium sp. FAKSA 10 under solid state fermentation using agro-industrial wastes |
| title_full | Evaluation of high-value bioproducts production by marine endophytic fungus Arthrinium sp. FAKSA 10 under solid state fermentation using agro-industrial wastes |
| title_fullStr | Evaluation of high-value bioproducts production by marine endophytic fungus Arthrinium sp. FAKSA 10 under solid state fermentation using agro-industrial wastes |
| title_full_unstemmed | Evaluation of high-value bioproducts production by marine endophytic fungus Arthrinium sp. FAKSA 10 under solid state fermentation using agro-industrial wastes |
| title_short | Evaluation of high-value bioproducts production by marine endophytic fungus Arthrinium sp. FAKSA 10 under solid state fermentation using agro-industrial wastes |
| title_sort | evaluation of high value bioproducts production by marine endophytic fungus arthrinium sp faksa 10 under solid state fermentation using agro industrial wastes |
| topic | Agro-industrial wastes Anticancer enzymes Anti-HCV Antimicrobial Antioxidant Arthrinium sp. |
| url | http://www.sciencedirect.com/science/article/pii/S0717345824000289 |
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