Effects of autologous dental pulp stem cells and mineral trioxide aggregate on exposed dogs’ dental pulp
This study evaluated exposed dogs’ dental pulp response after direct capping with autologous dental pulp stem cells (DPSCs) and compared the results with MTA (Angelus, Brazil). Sixty teeth from six mongrel dogs were included (n = 60), allocated into four groups; negative control (n = 6), positive co...
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| Format: | Article |
| Language: | English |
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Elsevier
2022-03-01
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| Series: | Journal of Oral Biology and Craniofacial Research |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2212426822000197 |
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| author | Dalia K. Hemdan Manar A. Selim Rania A. Galhom Mohamed H. El Daharawy Hayam Y. Hassan |
| author_facet | Dalia K. Hemdan Manar A. Selim Rania A. Galhom Mohamed H. El Daharawy Hayam Y. Hassan |
| author_sort | Dalia K. Hemdan |
| collection | DOAJ |
| description | This study evaluated exposed dogs’ dental pulp response after direct capping with autologous dental pulp stem cells (DPSCs) and compared the results with MTA (Angelus, Brazil). Sixty teeth from six mongrel dogs were included (n = 60), allocated into four groups; negative control (n = 6), positive control (n = 6), MTA (n = 24), and DPSCs (n = 24). DPSCs were isolated and cultured from extracted first premolars (1premolar/dog). The teeth were prepared for histological and immunohistochemical analyses. Statistical analysis was calculated using ANOVA tests, P ≤ 0.05. MTA induced the formation of an almost complete calcific bridge. DPSCs successfully regenerated the injured pulp and dentin. The comparison between the capping groups and time intervals had a statistically significant effect, as well as, the interaction between the two variables. DPSCs had greater ability in the reparative and regenerative process of dentin and odontoblastic differentiation by having a significantly stronger TGFβ1 expression than MTA. |
| format | Article |
| id | doaj-art-f1f7e4d078654a47b0082bc1e579d563 |
| institution | Kabale University |
| issn | 2212-4268 |
| language | English |
| publishDate | 2022-03-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Oral Biology and Craniofacial Research |
| spelling | doaj-art-f1f7e4d078654a47b0082bc1e579d5632024-11-23T06:29:12ZengElsevierJournal of Oral Biology and Craniofacial Research2212-42682022-03-01122293298Effects of autologous dental pulp stem cells and mineral trioxide aggregate on exposed dogs’ dental pulpDalia K. Hemdan0Manar A. Selim1Rania A. Galhom2Mohamed H. El Daharawy3Hayam Y. Hassan4Department of Endodontics, Faculty of Dentistry, Suez Canal University, Ismailia, Egypt; Corresponding author. Department of Endodontics, Faculty of Dentistry, Suez Canal University, 4.5 Km Ring Road, Ismailia, 41522, Egypt.Department of Oral Biology, Faculty of Dentistry, Suez Canal University, Ismailia, EgyptDepartment of Human Anatomy and Embryology, Faculty of Medicine, Suez Canal University, Ismailia, EgyptDepartment of Surgery, Anesthesiology and Radiology, Faculty of Veterinary Medicine, Suez Canal University, Ismailia, EgyptDepartment of Endodontics, Faculty of Dentistry, Suez Canal University, Ismailia, EgyptThis study evaluated exposed dogs’ dental pulp response after direct capping with autologous dental pulp stem cells (DPSCs) and compared the results with MTA (Angelus, Brazil). Sixty teeth from six mongrel dogs were included (n = 60), allocated into four groups; negative control (n = 6), positive control (n = 6), MTA (n = 24), and DPSCs (n = 24). DPSCs were isolated and cultured from extracted first premolars (1premolar/dog). The teeth were prepared for histological and immunohistochemical analyses. Statistical analysis was calculated using ANOVA tests, P ≤ 0.05. MTA induced the formation of an almost complete calcific bridge. DPSCs successfully regenerated the injured pulp and dentin. The comparison between the capping groups and time intervals had a statistically significant effect, as well as, the interaction between the two variables. DPSCs had greater ability in the reparative and regenerative process of dentin and odontoblastic differentiation by having a significantly stronger TGFβ1 expression than MTA.http://www.sciencedirect.com/science/article/pii/S2212426822000197Direct pulp cappingDPSCsMTATGFβ1Dogs' dental pulp |
| spellingShingle | Dalia K. Hemdan Manar A. Selim Rania A. Galhom Mohamed H. El Daharawy Hayam Y. Hassan Effects of autologous dental pulp stem cells and mineral trioxide aggregate on exposed dogs’ dental pulp Journal of Oral Biology and Craniofacial Research Direct pulp capping DPSCs MTA TGFβ1 Dogs' dental pulp |
| title | Effects of autologous dental pulp stem cells and mineral trioxide aggregate on exposed dogs’ dental pulp |
| title_full | Effects of autologous dental pulp stem cells and mineral trioxide aggregate on exposed dogs’ dental pulp |
| title_fullStr | Effects of autologous dental pulp stem cells and mineral trioxide aggregate on exposed dogs’ dental pulp |
| title_full_unstemmed | Effects of autologous dental pulp stem cells and mineral trioxide aggregate on exposed dogs’ dental pulp |
| title_short | Effects of autologous dental pulp stem cells and mineral trioxide aggregate on exposed dogs’ dental pulp |
| title_sort | effects of autologous dental pulp stem cells and mineral trioxide aggregate on exposed dogs dental pulp |
| topic | Direct pulp capping DPSCs MTA TGFβ1 Dogs' dental pulp |
| url | http://www.sciencedirect.com/science/article/pii/S2212426822000197 |
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