Growth maturity stage defines nutritional, anti-nutrients and functional food aspects of Moringa oleifera Lam. leaves

This study evaluates nutritional composition and anti-nutrients profile of Moringa oleifera leaves across different growth stages aimed at identifying optimum harvest time to secure better dietary profile of the leaves. The results showed that physicochemical composition of mature leaves present a s...

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Main Authors: Saima Perveen, Saeed Akhtar, Dur-e-shahwar Sattar, Muhammad Qamar, Wisha Saeed, Mavra Amin, Tariq Ismail
Format: Article
Language:English
Published: Elsevier 2025-06-01
Series:Food Chemistry Advances
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Online Access:http://www.sciencedirect.com/science/article/pii/S2772753X25001261
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author Saima Perveen
Saeed Akhtar
Dur-e-shahwar Sattar
Muhammad Qamar
Wisha Saeed
Mavra Amin
Tariq Ismail
author_facet Saima Perveen
Saeed Akhtar
Dur-e-shahwar Sattar
Muhammad Qamar
Wisha Saeed
Mavra Amin
Tariq Ismail
author_sort Saima Perveen
collection DOAJ
description This study evaluates nutritional composition and anti-nutrients profile of Moringa oleifera leaves across different growth stages aimed at identifying optimum harvest time to secure better dietary profile of the leaves. The results showed that physicochemical composition of mature leaves present a significantly (p ≤ 0.05) higher level of protein (23.71 %), fiber (28.26 %), and fat (13.26 %) with a relatively lower amount of caloric energy (271.93 kcal) as compared to those observed in tender and young leaves. Progressive reduction (p ≤ 0.05) was noticed in anthocyanin (31.96 to 14.31 mg/100 g), chlorophyll b (72.83 to 45.16 mg/100 g) and lycopene (22.67 to 11.94 mg/100 g) content of M. oleifera leaves with advancing growth stage. Growth stages depicted an increase in the β-carotene and chlorophyll A contents of M. oleifera leaves. Higher phenolics yield and antioxidant activity of the leaves extracts was recorded for methanolic extracts. Advanced stage of leaves maturity anticipated highest level of total phenolics i.e., 49.02 mg GAE/g, total flavonoids content i.e., 52.96 mg QE/g and flavonols (28.83 mg QE/g). Comparatively higher concentrations of alkaloids (2.74 g/100 g), saponin (3.82 g/100 g), oxalates (2.43 g/100 g), tannin (0.837 g/100 g), and phytic acid (1.94 g/100 g) content were recorded in mature leaves. Harvesting M. oleifera leaves at peak maturity enhances their protein, dietary fiber, micronutrient, and phytochemical content. However, mature leaves may also accumulate higher levels of anti-nutrients like oxalates and phytates, which can interfere with mineral absorption.
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spelling doaj-art-f1883c36cab847a5a94f0e1a69859dc72025-08-20T03:45:08ZengElsevierFood Chemistry Advances2772-753X2025-06-01710101010.1016/j.focha.2025.101010Growth maturity stage defines nutritional, anti-nutrients and functional food aspects of Moringa oleifera Lam. leavesSaima Perveen0Saeed Akhtar1Dur-e-shahwar Sattar2Muhammad Qamar3Wisha Saeed4Mavra Amin5Tariq Ismail6Department of Food Science & Technology, Faculty of Food Science & Nutrition, Bahauddin Zakariya University, Multan 60800, PakistanDepartment of Food Science & Technology, Faculty of Food Science & Nutrition, Bahauddin Zakariya University, Multan 60800, PakistanDepartment of Food Science & Technology, Faculty of Food Science & Nutrition, Bahauddin Zakariya University, Multan 60800, PakistanDepartment of Food Science & Technology, Faculty of Food Science & Nutrition, Bahauddin Zakariya University, Multan 60800, PakistanDepartment of Food Science & Technology, Faculty of Food Science & Nutrition, Bahauddin Zakariya University, Multan 60800, PakistanDepartment of Food Science & Technology, Faculty of Food Science & Nutrition, Bahauddin Zakariya University, Multan 60800, PakistanCorresponding author.; Department of Food Science & Technology, Faculty of Food Science & Nutrition, Bahauddin Zakariya University, Multan 60800, PakistanThis study evaluates nutritional composition and anti-nutrients profile of Moringa oleifera leaves across different growth stages aimed at identifying optimum harvest time to secure better dietary profile of the leaves. The results showed that physicochemical composition of mature leaves present a significantly (p ≤ 0.05) higher level of protein (23.71 %), fiber (28.26 %), and fat (13.26 %) with a relatively lower amount of caloric energy (271.93 kcal) as compared to those observed in tender and young leaves. Progressive reduction (p ≤ 0.05) was noticed in anthocyanin (31.96 to 14.31 mg/100 g), chlorophyll b (72.83 to 45.16 mg/100 g) and lycopene (22.67 to 11.94 mg/100 g) content of M. oleifera leaves with advancing growth stage. Growth stages depicted an increase in the β-carotene and chlorophyll A contents of M. oleifera leaves. Higher phenolics yield and antioxidant activity of the leaves extracts was recorded for methanolic extracts. Advanced stage of leaves maturity anticipated highest level of total phenolics i.e., 49.02 mg GAE/g, total flavonoids content i.e., 52.96 mg QE/g and flavonols (28.83 mg QE/g). Comparatively higher concentrations of alkaloids (2.74 g/100 g), saponin (3.82 g/100 g), oxalates (2.43 g/100 g), tannin (0.837 g/100 g), and phytic acid (1.94 g/100 g) content were recorded in mature leaves. Harvesting M. oleifera leaves at peak maturity enhances their protein, dietary fiber, micronutrient, and phytochemical content. However, mature leaves may also accumulate higher levels of anti-nutrients like oxalates and phytates, which can interfere with mineral absorption.http://www.sciencedirect.com/science/article/pii/S2772753X25001261Moringa leavesGrowth maturity stageAnti-nutrientsProteinOxalatesBioactive compounds
spellingShingle Saima Perveen
Saeed Akhtar
Dur-e-shahwar Sattar
Muhammad Qamar
Wisha Saeed
Mavra Amin
Tariq Ismail
Growth maturity stage defines nutritional, anti-nutrients and functional food aspects of Moringa oleifera Lam. leaves
Food Chemistry Advances
Moringa leaves
Growth maturity stage
Anti-nutrients
Protein
Oxalates
Bioactive compounds
title Growth maturity stage defines nutritional, anti-nutrients and functional food aspects of Moringa oleifera Lam. leaves
title_full Growth maturity stage defines nutritional, anti-nutrients and functional food aspects of Moringa oleifera Lam. leaves
title_fullStr Growth maturity stage defines nutritional, anti-nutrients and functional food aspects of Moringa oleifera Lam. leaves
title_full_unstemmed Growth maturity stage defines nutritional, anti-nutrients and functional food aspects of Moringa oleifera Lam. leaves
title_short Growth maturity stage defines nutritional, anti-nutrients and functional food aspects of Moringa oleifera Lam. leaves
title_sort growth maturity stage defines nutritional anti nutrients and functional food aspects of moringa oleifera lam leaves
topic Moringa leaves
Growth maturity stage
Anti-nutrients
Protein
Oxalates
Bioactive compounds
url http://www.sciencedirect.com/science/article/pii/S2772753X25001261
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