Obtaining and Characterization of Hybridomas Producing Monoclonal Antibodies to Shiga-Like Toxins of I and II Types
Objective – obtaining and characterization of hybrid cell lines producing monoclonal antibodies against I and II types of shiga-like toxins.Materials and methods. Shiga-like toxins obtained in “48thCentral Research Institute” of Ministry of Defense of Russian Federation (Kirov), BALB/c mice, myeloma...
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| Format: | Article |
| Language: | Russian |
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Federal Government Health Institution, Russian Research Anti-Plague Institute “Microbe”
2021-10-01
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| Series: | Проблемы особо опасных инфекций |
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| Online Access: | https://journal.microbe.ru/jour/article/view/1551 |
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| author | G. V. Kuklina S. S. Ipatov D. V. Pechenkin A. V. Eremkin A. A. Kytmanov M. Yu. Dubrovin A. S. Gorshkov |
| author_facet | G. V. Kuklina S. S. Ipatov D. V. Pechenkin A. V. Eremkin A. A. Kytmanov M. Yu. Dubrovin A. S. Gorshkov |
| author_sort | G. V. Kuklina |
| collection | DOAJ |
| description | Objective – obtaining and characterization of hybrid cell lines producing monoclonal antibodies against I and II types of shiga-like toxins.Materials and methods. Shiga-like toxins obtained in “48thCentral Research Institute” of Ministry of Defense of Russian Federation (Kirov), BALB/c mice, myeloma cells SP2/0-Ag14 were used in research. Immune splenocytes and SP2/0-Ag14 myeloma cells were fused according to G. Kohler and C. Milstein method in De St. Fazekas and D. Scheidegger modifcation using 50 % polyethylene glycol. Hybrid cell lines producing specifc monoclonal antibodies were cloned by limited dilutions. Hybridomas growth and producing properties were studied in vitro and in vivo. Specifc activity of immune sera, culture and ascitic fluids were studied by indirect ELISA. Monoclonal antibodies from ascitic fluids were precipitated by saturated ammonium sulfate, followed by ion exchange chromatographyResults and discussion. 8 hybridomas producing monoclonal antibodies against I and II types shiga-like toxins were obtained. Hybridomas are characterized by stable proliferation and antibody-producing activity during 10 passages in vitro and 3 passages in vivo (observation period). Obtained monoclonal antibodies can be used for ELISA detection of I and II types shiga-like toxins. Minimum detectable concentration of shiga-like toxins in sandwich ELISA is 1 ng/ml. The possibility of detecting shiga-like toxins without typical differentiation was shown when using in the enzyme immunoassay a polyreceptor mixture of monoclonal antibodies for sensitizing the plate and a polyspecifc mixture of immunoperoxidase conjugates. |
| format | Article |
| id | doaj-art-f17fb4e948044bb08d25f28b1ecb2e1f |
| institution | Kabale University |
| issn | 0370-1069 2658-719X |
| language | Russian |
| publishDate | 2021-10-01 |
| publisher | Federal Government Health Institution, Russian Research Anti-Plague Institute “Microbe” |
| record_format | Article |
| series | Проблемы особо опасных инфекций |
| spelling | doaj-art-f17fb4e948044bb08d25f28b1ecb2e1f2025-08-20T03:50:11ZrusFederal Government Health Institution, Russian Research Anti-Plague Institute “Microbe”Проблемы особо опасных инфекций0370-10692658-719X2021-10-0103838810.21055/0370-1069-2021-3-83-881312Obtaining and Characterization of Hybridomas Producing Monoclonal Antibodies to Shiga-Like Toxins of I and II TypesG. V. Kuklina0S. S. Ipatov1D. V. Pechenkin2A. V. Eremkin3A. A. Kytmanov4M. Yu. Dubrovin5A. S. Gorshkov6Affiliated Branch of the Federal State Budgetary Institution “48th Central Research Institute” of the Ministry of Defense of the Russian FederationAffiliated Branch of the Federal State Budgetary Institution “48th Central Research Institute” of the Ministry of Defense of the Russian FederationAffiliated Branch of the Federal State Budgetary Institution “48th Central Research Institute” of the Ministry of Defense of the Russian FederationAffiliated Branch of the Federal State Budgetary Institution “48th Central Research Institute” of the Ministry of Defense of the Russian FederationAffiliated Branch of the Federal State Budgetary Institution “48th Central Research Institute” of the Ministry of Defense of the Russian FederationAffiliated Branch of the Federal State Budgetary Institution “48th Central Research Institute” of the Ministry of Defense of the Russian FederationAffiliated Branch of the Federal State Budgetary Institution “48th Central Research Institute” of the Ministry of Defense of the Russian FederationObjective – obtaining and characterization of hybrid cell lines producing monoclonal antibodies against I and II types of shiga-like toxins.Materials and methods. Shiga-like toxins obtained in “48thCentral Research Institute” of Ministry of Defense of Russian Federation (Kirov), BALB/c mice, myeloma cells SP2/0-Ag14 were used in research. Immune splenocytes and SP2/0-Ag14 myeloma cells were fused according to G. Kohler and C. Milstein method in De St. Fazekas and D. Scheidegger modifcation using 50 % polyethylene glycol. Hybrid cell lines producing specifc monoclonal antibodies were cloned by limited dilutions. Hybridomas growth and producing properties were studied in vitro and in vivo. Specifc activity of immune sera, culture and ascitic fluids were studied by indirect ELISA. Monoclonal antibodies from ascitic fluids were precipitated by saturated ammonium sulfate, followed by ion exchange chromatographyResults and discussion. 8 hybridomas producing monoclonal antibodies against I and II types shiga-like toxins were obtained. Hybridomas are characterized by stable proliferation and antibody-producing activity during 10 passages in vitro and 3 passages in vivo (observation period). Obtained monoclonal antibodies can be used for ELISA detection of I and II types shiga-like toxins. Minimum detectable concentration of shiga-like toxins in sandwich ELISA is 1 ng/ml. The possibility of detecting shiga-like toxins without typical differentiation was shown when using in the enzyme immunoassay a polyreceptor mixture of monoclonal antibodies for sensitizing the plate and a polyspecifc mixture of immunoperoxidase conjugates.https://journal.microbe.ru/jour/article/view/1551shiga-like toxinsmonoclonal antibodiesand enzyme linked immunosorbent assay |
| spellingShingle | G. V. Kuklina S. S. Ipatov D. V. Pechenkin A. V. Eremkin A. A. Kytmanov M. Yu. Dubrovin A. S. Gorshkov Obtaining and Characterization of Hybridomas Producing Monoclonal Antibodies to Shiga-Like Toxins of I and II Types Проблемы особо опасных инфекций shiga-like toxins monoclonal antibodies and enzyme linked immunosorbent assay |
| title | Obtaining and Characterization of Hybridomas Producing Monoclonal Antibodies to Shiga-Like Toxins of I and II Types |
| title_full | Obtaining and Characterization of Hybridomas Producing Monoclonal Antibodies to Shiga-Like Toxins of I and II Types |
| title_fullStr | Obtaining and Characterization of Hybridomas Producing Monoclonal Antibodies to Shiga-Like Toxins of I and II Types |
| title_full_unstemmed | Obtaining and Characterization of Hybridomas Producing Monoclonal Antibodies to Shiga-Like Toxins of I and II Types |
| title_short | Obtaining and Characterization of Hybridomas Producing Monoclonal Antibodies to Shiga-Like Toxins of I and II Types |
| title_sort | obtaining and characterization of hybridomas producing monoclonal antibodies to shiga like toxins of i and ii types |
| topic | shiga-like toxins monoclonal antibodies and enzyme linked immunosorbent assay |
| url | https://journal.microbe.ru/jour/article/view/1551 |
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