Short-Term Changes in Fecal Bacteriobiome of Healthy Laboratory Mice After Antiviral Preparation Administration
Backgound/Objective: Novel compounds for mitigating globally growing microbial resistance to antibiotics have been recently more actively researched. Triviron is a polycationic amphiphile synthetic compound with a ribonuclease activity and is used as an antiviral in veterinary medicine. Methods: We...
Saved in:
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2024-12-01
|
| Series: | Drugs and Drug Candidates |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2813-2998/3/4/49 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1846105104484139008 |
|---|---|
| author | Olga Baturina Natalia Naumova Alexey Tupikin Elena Dmitrienko Vladimir Silnikov Marsel Kabilov |
| author_facet | Olga Baturina Natalia Naumova Alexey Tupikin Elena Dmitrienko Vladimir Silnikov Marsel Kabilov |
| author_sort | Olga Baturina |
| collection | DOAJ |
| description | Backgound/Objective: Novel compounds for mitigating globally growing microbial resistance to antibiotics have been recently more actively researched. Triviron is a polycationic amphiphile synthetic compound with a ribonuclease activity and is used as an antiviral in veterinary medicine. Methods: We studied the effect of triviron on the mouse (line Balb/c) fecal bacteriobiome at different time points (0, 5, 25, and 120 h after a single intragastrical administration) by using amplicon sequence diversity of the V3/V4 region of 16S rRNA genes. Results: Most of the operational taxonomic units (OTUs) belonged to <i>Bacillota</i> (1168 OTUs, i.e., 56% of the total number of OTUs in the study) and <i>Bacteroidota</i> (354, i.e., 17%), with the phyla together accounting for more than 90% of the total number of sequence reads. We found changed relative abundance of some bacterial taxa with time, including the dominating <i>Bacteroidota</i> and <i>Bacillota</i> phyla; some of the changes were sex-related, although at the start of the experiment, there were no difference between the sexes in their fecal bacteriobiome composition and structure. Conclusions: The results unequivocally demonstrated that in mice, feces bacterial community structure was affected by a one-time triviron administration, even at the highest hierarchical level of phyla. The finding that the core dominant phyla can be affected, with the effect lasting at least for five days, implies that some major and important functions of the gut microbiota can be affected as well. |
| format | Article |
| id | doaj-art-f16e70fc53e54e74b7459a4b05c4aae3 |
| institution | Kabale University |
| issn | 2813-2998 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Drugs and Drug Candidates |
| spelling | doaj-art-f16e70fc53e54e74b7459a4b05c4aae32024-12-27T14:20:17ZengMDPI AGDrugs and Drug Candidates2813-29982024-12-013487988910.3390/ddc3040049Short-Term Changes in Fecal Bacteriobiome of Healthy Laboratory Mice After Antiviral Preparation AdministrationOlga Baturina0Natalia Naumova1Alexey Tupikin2Elena Dmitrienko3Vladimir Silnikov4Marsel Kabilov5Institute of Chemical Biology and Fundamental Medicine of the Siberian Branch, Russian Academy of Sciences, 630090 Novosibirsk, RussiaInstitute of Chemical Biology and Fundamental Medicine of the Siberian Branch, Russian Academy of Sciences, 630090 Novosibirsk, RussiaInstitute of Chemical Biology and Fundamental Medicine of the Siberian Branch, Russian Academy of Sciences, 630090 Novosibirsk, RussiaInstitute of Chemical Biology and Fundamental Medicine of the Siberian Branch, Russian Academy of Sciences, 630090 Novosibirsk, RussiaInstitute of Chemical Biology and Fundamental Medicine of the Siberian Branch, Russian Academy of Sciences, 630090 Novosibirsk, RussiaInstitute of Chemical Biology and Fundamental Medicine of the Siberian Branch, Russian Academy of Sciences, 630090 Novosibirsk, RussiaBackgound/Objective: Novel compounds for mitigating globally growing microbial resistance to antibiotics have been recently more actively researched. Triviron is a polycationic amphiphile synthetic compound with a ribonuclease activity and is used as an antiviral in veterinary medicine. Methods: We studied the effect of triviron on the mouse (line Balb/c) fecal bacteriobiome at different time points (0, 5, 25, and 120 h after a single intragastrical administration) by using amplicon sequence diversity of the V3/V4 region of 16S rRNA genes. Results: Most of the operational taxonomic units (OTUs) belonged to <i>Bacillota</i> (1168 OTUs, i.e., 56% of the total number of OTUs in the study) and <i>Bacteroidota</i> (354, i.e., 17%), with the phyla together accounting for more than 90% of the total number of sequence reads. We found changed relative abundance of some bacterial taxa with time, including the dominating <i>Bacteroidota</i> and <i>Bacillota</i> phyla; some of the changes were sex-related, although at the start of the experiment, there were no difference between the sexes in their fecal bacteriobiome composition and structure. Conclusions: The results unequivocally demonstrated that in mice, feces bacterial community structure was affected by a one-time triviron administration, even at the highest hierarchical level of phyla. The finding that the core dominant phyla can be affected, with the effect lasting at least for five days, implies that some major and important functions of the gut microbiota can be affected as well.https://www.mdpi.com/2813-2998/3/4/4916S rRNA genebacterial diversitytrivirongut microbiomemouse line Balb/c |
| spellingShingle | Olga Baturina Natalia Naumova Alexey Tupikin Elena Dmitrienko Vladimir Silnikov Marsel Kabilov Short-Term Changes in Fecal Bacteriobiome of Healthy Laboratory Mice After Antiviral Preparation Administration Drugs and Drug Candidates 16S rRNA gene bacterial diversity triviron gut microbiome mouse line Balb/c |
| title | Short-Term Changes in Fecal Bacteriobiome of Healthy Laboratory Mice After Antiviral Preparation Administration |
| title_full | Short-Term Changes in Fecal Bacteriobiome of Healthy Laboratory Mice After Antiviral Preparation Administration |
| title_fullStr | Short-Term Changes in Fecal Bacteriobiome of Healthy Laboratory Mice After Antiviral Preparation Administration |
| title_full_unstemmed | Short-Term Changes in Fecal Bacteriobiome of Healthy Laboratory Mice After Antiviral Preparation Administration |
| title_short | Short-Term Changes in Fecal Bacteriobiome of Healthy Laboratory Mice After Antiviral Preparation Administration |
| title_sort | short term changes in fecal bacteriobiome of healthy laboratory mice after antiviral preparation administration |
| topic | 16S rRNA gene bacterial diversity triviron gut microbiome mouse line Balb/c |
| url | https://www.mdpi.com/2813-2998/3/4/49 |
| work_keys_str_mv | AT olgabaturina shorttermchangesinfecalbacteriobiomeofhealthylaboratorymiceafterantiviralpreparationadministration AT natalianaumova shorttermchangesinfecalbacteriobiomeofhealthylaboratorymiceafterantiviralpreparationadministration AT alexeytupikin shorttermchangesinfecalbacteriobiomeofhealthylaboratorymiceafterantiviralpreparationadministration AT elenadmitrienko shorttermchangesinfecalbacteriobiomeofhealthylaboratorymiceafterantiviralpreparationadministration AT vladimirsilnikov shorttermchangesinfecalbacteriobiomeofhealthylaboratorymiceafterantiviralpreparationadministration AT marselkabilov shorttermchangesinfecalbacteriobiomeofhealthylaboratorymiceafterantiviralpreparationadministration |