Comparing rumen fluid collection methods on fermentation profile and microbial population in lactating dairy cows
The objective of this study was to compare fermentation profile and microbial diversity from rumen samples collected using a rumen cannula (RC) or stomach tube (ST) in lactating dairy cows. Three ruminally cannulated lactating dairy cows were used in a 3 × 3 Latin square design. The experimental per...
Saved in:
| Main Authors: | , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2025-01-01
|
| Series: | JDS Communications |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2666910224001091 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1841550729173532672 |
|---|---|
| author | N. Pathak H. Guan P. Fan H. Sultana K. Arriola A. Oyebade C. Nino de Guzman M. Malekkhahi K.C. Jeong D. Vyas |
| author_facet | N. Pathak H. Guan P. Fan H. Sultana K. Arriola A. Oyebade C. Nino de Guzman M. Malekkhahi K.C. Jeong D. Vyas |
| author_sort | N. Pathak |
| collection | DOAJ |
| description | The objective of this study was to compare fermentation profile and microbial diversity from rumen samples collected using a rumen cannula (RC) or stomach tube (ST) in lactating dairy cows. Three ruminally cannulated lactating dairy cows were used in a 3 × 3 Latin square design. The experimental period was 28 d and rumen fluid was collected 4 h after feeding on d 22 and 26 of each experimental period. Treatments were rumen fluid collected from the ST or RC. The pH of rumen contents was measured immediately after fluid collection and samples were analyzed for VFA, ammonia-N (NH3-N) concentration, and microbiome composition. Rumen pH was greater for ST compared with RC (6.88 vs. 6.25). However, NH3-N (15.2 vs. 10.6 mg/dL) and total VFA (121.8 vs. 95.5 mM) were greater for RC compared with ST. The rumen fluid collection methods had no effects on the molar proportion of acetate and propionate; however, the acetate-to-propionate ratio tended to increase with ST compared with RC. The majority of sequences obtained with microbiome analysis belonged to Bacteroidetes, Firmicutes, and Cyanobacteria, regardless of the rumen fluid collection method. However, the abundance of Bacteroidetes was greater and the abundance of Firmicutes was lower for ST compared with RC. No effects were observed on the abundance of phylum Cyanobacteria. The rumen fluid collection methods had no effects on Chao1 and Shannon index. In conclusion, the molar proportion of individual VFA, Chao1, and Shannon index were similar, whereas rumen pH, NH3-N, and total VFA were affected by the rumen fluid collection method. |
| format | Article |
| id | doaj-art-f16cb27f207d4b448c3c1799b114d24f |
| institution | Kabale University |
| issn | 2666-9102 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | JDS Communications |
| spelling | doaj-art-f16cb27f207d4b448c3c1799b114d24f2025-01-10T04:38:08ZengElsevierJDS Communications2666-91022025-01-01613438Comparing rumen fluid collection methods on fermentation profile and microbial population in lactating dairy cowsN. Pathak0H. Guan1P. Fan2H. Sultana3K. Arriola4A. Oyebade5C. Nino de Guzman6M. Malekkhahi7K.C. Jeong8D. Vyas9Department of Animal Science, University of Florida, Gainesville, FL 32611Department of Animal Science, University of Florida, Gainesville, FL 32611Department of Animal Science, University of Florida, Gainesville, FL 32611Department of Animal Science, University of Florida, Gainesville, FL 32611Department of Animal Science, University of Florida, Gainesville, FL 32611Department of Animal Science, University of Florida, Gainesville, FL 32611Department of Animal Science, University of Florida, Gainesville, FL 32611Department of Animal Science, University of Florida, Gainesville, FL 32611Department of Animal Science, University of Florida, Gainesville, FL 32611Corresponding author; Department of Animal Science, University of Florida, Gainesville, FL 32611The objective of this study was to compare fermentation profile and microbial diversity from rumen samples collected using a rumen cannula (RC) or stomach tube (ST) in lactating dairy cows. Three ruminally cannulated lactating dairy cows were used in a 3 × 3 Latin square design. The experimental period was 28 d and rumen fluid was collected 4 h after feeding on d 22 and 26 of each experimental period. Treatments were rumen fluid collected from the ST or RC. The pH of rumen contents was measured immediately after fluid collection and samples were analyzed for VFA, ammonia-N (NH3-N) concentration, and microbiome composition. Rumen pH was greater for ST compared with RC (6.88 vs. 6.25). However, NH3-N (15.2 vs. 10.6 mg/dL) and total VFA (121.8 vs. 95.5 mM) were greater for RC compared with ST. The rumen fluid collection methods had no effects on the molar proportion of acetate and propionate; however, the acetate-to-propionate ratio tended to increase with ST compared with RC. The majority of sequences obtained with microbiome analysis belonged to Bacteroidetes, Firmicutes, and Cyanobacteria, regardless of the rumen fluid collection method. However, the abundance of Bacteroidetes was greater and the abundance of Firmicutes was lower for ST compared with RC. No effects were observed on the abundance of phylum Cyanobacteria. The rumen fluid collection methods had no effects on Chao1 and Shannon index. In conclusion, the molar proportion of individual VFA, Chao1, and Shannon index were similar, whereas rumen pH, NH3-N, and total VFA were affected by the rumen fluid collection method.http://www.sciencedirect.com/science/article/pii/S2666910224001091 |
| spellingShingle | N. Pathak H. Guan P. Fan H. Sultana K. Arriola A. Oyebade C. Nino de Guzman M. Malekkhahi K.C. Jeong D. Vyas Comparing rumen fluid collection methods on fermentation profile and microbial population in lactating dairy cows JDS Communications |
| title | Comparing rumen fluid collection methods on fermentation profile and microbial population in lactating dairy cows |
| title_full | Comparing rumen fluid collection methods on fermentation profile and microbial population in lactating dairy cows |
| title_fullStr | Comparing rumen fluid collection methods on fermentation profile and microbial population in lactating dairy cows |
| title_full_unstemmed | Comparing rumen fluid collection methods on fermentation profile and microbial population in lactating dairy cows |
| title_short | Comparing rumen fluid collection methods on fermentation profile and microbial population in lactating dairy cows |
| title_sort | comparing rumen fluid collection methods on fermentation profile and microbial population in lactating dairy cows |
| url | http://www.sciencedirect.com/science/article/pii/S2666910224001091 |
| work_keys_str_mv | AT npathak comparingrumenfluidcollectionmethodsonfermentationprofileandmicrobialpopulationinlactatingdairycows AT hguan comparingrumenfluidcollectionmethodsonfermentationprofileandmicrobialpopulationinlactatingdairycows AT pfan comparingrumenfluidcollectionmethodsonfermentationprofileandmicrobialpopulationinlactatingdairycows AT hsultana comparingrumenfluidcollectionmethodsonfermentationprofileandmicrobialpopulationinlactatingdairycows AT karriola comparingrumenfluidcollectionmethodsonfermentationprofileandmicrobialpopulationinlactatingdairycows AT aoyebade comparingrumenfluidcollectionmethodsonfermentationprofileandmicrobialpopulationinlactatingdairycows AT cninodeguzman comparingrumenfluidcollectionmethodsonfermentationprofileandmicrobialpopulationinlactatingdairycows AT mmalekkhahi comparingrumenfluidcollectionmethodsonfermentationprofileandmicrobialpopulationinlactatingdairycows AT kcjeong comparingrumenfluidcollectionmethodsonfermentationprofileandmicrobialpopulationinlactatingdairycows AT dvyas comparingrumenfluidcollectionmethodsonfermentationprofileandmicrobialpopulationinlactatingdairycows |