Generation of two isogenic human iPSC lines (ZIPi013-B-1, ZIPi013-B-2) carrying a CRISPR/Cas9-mediated deletion of TRPM4
Two isogenic hiPSC lines, ZIPi013-B-1 and ZIPi013-B-2, were generated by CRISPR/Cas9-mediated indels in the TRPM4 gene of the previously published ZIPi013-B. TRPM4 belongs to the evolutionarily conserved family of transient receptor potential (TRP) channels. It is expressed ubiquitously and its acti...
Saved in:
| Main Authors: | , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2024-12-01
|
| Series: | Stem Cell Research |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S1873506124003076 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1846125510501859328 |
|---|---|
| author | Undine Haferkamp Narasimha Telugu Kim Krieg Wiebe Schaefer Dennis Lam Lars Binkle-Ladisch Manuel A. Friese Sebastian Diecke Ole Pless |
| author_facet | Undine Haferkamp Narasimha Telugu Kim Krieg Wiebe Schaefer Dennis Lam Lars Binkle-Ladisch Manuel A. Friese Sebastian Diecke Ole Pless |
| author_sort | Undine Haferkamp |
| collection | DOAJ |
| description | Two isogenic hiPSC lines, ZIPi013-B-1 and ZIPi013-B-2, were generated by CRISPR/Cas9-mediated indels in the TRPM4 gene of the previously published ZIPi013-B. TRPM4 belongs to the evolutionarily conserved family of transient receptor potential (TRP) channels. It is expressed ubiquitously and its activity is regulated by intracellular calcium binding, changes in membrane potential, phosphoinositide lipids in the plasma membrane and the local concentration of cytoplasmic ATP and ADP. TRPM4 has been implicated in various diseases, including neurological and immune system disorders, cardiac diseases and cancer. Both new cell lines offer the opportunity to model human diseases and test therapeutic modalities addressing these. |
| format | Article |
| id | doaj-art-f15cd7cb53b64c50afe05c17fc33c3ae |
| institution | Kabale University |
| issn | 1873-5061 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Stem Cell Research |
| spelling | doaj-art-f15cd7cb53b64c50afe05c17fc33c3ae2024-12-13T10:57:00ZengElsevierStem Cell Research1873-50612024-12-0181103609Generation of two isogenic human iPSC lines (ZIPi013-B-1, ZIPi013-B-2) carrying a CRISPR/Cas9-mediated deletion of TRPM4Undine Haferkamp0Narasimha Telugu1Kim Krieg2Wiebe Schaefer3Dennis Lam4Lars Binkle-Ladisch5Manuel A. Friese6Sebastian Diecke7Ole Pless8Fraunhofer Institute for Translational Medicine and Pharmacology ITPM, Discovery Research ScreeningPort, Hamburg, GermanyMax-Delbrück-Center for Molecular Medicine in the Helmholtz Association (MDC), Technology Platform Pluripotent Stem Cells, Berlin, GermanyFraunhofer Institute for Translational Medicine and Pharmacology ITPM, Discovery Research ScreeningPort, Hamburg, GermanyFraunhofer Institute for Translational Medicine and Pharmacology ITPM, Discovery Research ScreeningPort, Hamburg, GermanyFraunhofer Institute for Translational Medicine and Pharmacology ITPM, Discovery Research ScreeningPort, Hamburg, GermanyUniversity Medical Center Hamburg-Eppendorf, Institute of Neuroimmunology and Multiple Sclerosis, Hamburg, GermanyUniversity Medical Center Hamburg-Eppendorf, Institute of Neuroimmunology and Multiple Sclerosis, Hamburg, GermanyMax-Delbrück-Center for Molecular Medicine in the Helmholtz Association (MDC), Technology Platform Pluripotent Stem Cells, Berlin, GermanyFraunhofer Institute for Translational Medicine and Pharmacology ITPM, Discovery Research ScreeningPort, Hamburg, Germany; Corresponding author.Two isogenic hiPSC lines, ZIPi013-B-1 and ZIPi013-B-2, were generated by CRISPR/Cas9-mediated indels in the TRPM4 gene of the previously published ZIPi013-B. TRPM4 belongs to the evolutionarily conserved family of transient receptor potential (TRP) channels. It is expressed ubiquitously and its activity is regulated by intracellular calcium binding, changes in membrane potential, phosphoinositide lipids in the plasma membrane and the local concentration of cytoplasmic ATP and ADP. TRPM4 has been implicated in various diseases, including neurological and immune system disorders, cardiac diseases and cancer. Both new cell lines offer the opportunity to model human diseases and test therapeutic modalities addressing these.http://www.sciencedirect.com/science/article/pii/S1873506124003076 |
| spellingShingle | Undine Haferkamp Narasimha Telugu Kim Krieg Wiebe Schaefer Dennis Lam Lars Binkle-Ladisch Manuel A. Friese Sebastian Diecke Ole Pless Generation of two isogenic human iPSC lines (ZIPi013-B-1, ZIPi013-B-2) carrying a CRISPR/Cas9-mediated deletion of TRPM4 Stem Cell Research |
| title | Generation of two isogenic human iPSC lines (ZIPi013-B-1, ZIPi013-B-2) carrying a CRISPR/Cas9-mediated deletion of TRPM4 |
| title_full | Generation of two isogenic human iPSC lines (ZIPi013-B-1, ZIPi013-B-2) carrying a CRISPR/Cas9-mediated deletion of TRPM4 |
| title_fullStr | Generation of two isogenic human iPSC lines (ZIPi013-B-1, ZIPi013-B-2) carrying a CRISPR/Cas9-mediated deletion of TRPM4 |
| title_full_unstemmed | Generation of two isogenic human iPSC lines (ZIPi013-B-1, ZIPi013-B-2) carrying a CRISPR/Cas9-mediated deletion of TRPM4 |
| title_short | Generation of two isogenic human iPSC lines (ZIPi013-B-1, ZIPi013-B-2) carrying a CRISPR/Cas9-mediated deletion of TRPM4 |
| title_sort | generation of two isogenic human ipsc lines zipi013 b 1 zipi013 b 2 carrying a crispr cas9 mediated deletion of trpm4 |
| url | http://www.sciencedirect.com/science/article/pii/S1873506124003076 |
| work_keys_str_mv | AT undinehaferkamp generationoftwoisogenichumanipsclineszipi013b1zipi013b2carryingacrisprcas9mediateddeletionoftrpm4 AT narasimhatelugu generationoftwoisogenichumanipsclineszipi013b1zipi013b2carryingacrisprcas9mediateddeletionoftrpm4 AT kimkrieg generationoftwoisogenichumanipsclineszipi013b1zipi013b2carryingacrisprcas9mediateddeletionoftrpm4 AT wiebeschaefer generationoftwoisogenichumanipsclineszipi013b1zipi013b2carryingacrisprcas9mediateddeletionoftrpm4 AT dennislam generationoftwoisogenichumanipsclineszipi013b1zipi013b2carryingacrisprcas9mediateddeletionoftrpm4 AT larsbinkleladisch generationoftwoisogenichumanipsclineszipi013b1zipi013b2carryingacrisprcas9mediateddeletionoftrpm4 AT manuelafriese generationoftwoisogenichumanipsclineszipi013b1zipi013b2carryingacrisprcas9mediateddeletionoftrpm4 AT sebastiandiecke generationoftwoisogenichumanipsclineszipi013b1zipi013b2carryingacrisprcas9mediateddeletionoftrpm4 AT olepless generationoftwoisogenichumanipsclineszipi013b1zipi013b2carryingacrisprcas9mediateddeletionoftrpm4 |